Embryo transfer in cattle

Barros, C. M.; Nogueira, Marcelo Fábio Gouveia

doi:10.1016/s0093-691x(01)00648-3

Cited by 60 publications

(31 citation statements)

References 76 publications

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“…Total COC means in the present trial were smaller to those reported in Zebu cows [16, 17]. The number of oocytes was always lower than the AFC, which may reflect losses due to aspiration techniques and methodology, although this is commonly found in the literature [18, 19]. The same is true in relation to embryo recovery and corpora lutea counts in embryo transfer reports [20, 21].…”

Section: Resultscontrasting

confidence: 45%

Vulvar width and rima length as predictors of the ovarian follicular reserve in bovine females

Mesquita

Maculan

Maciel

et al. 2016

Journal of Reproduction and Development

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The aim of this study was to investigate possible relationships between morphometric characteristics of the bovine reproductive tract and measures of fertility, such as antral follicle counts (AFCs) and the number and quality of recovered cumulus oophorus complexes (COCs). First, the genital tracts of 360 abattoir Zebu/Holstein crossbred cows were studied. Rima vulvae length (RL), vulvar width (VW), ovarian size, pelvic fat, number of aspirated COCs, and AFCs were recorded. An index of COC quality (CQI) was established, which weighed overall gamete quality based on the IETS classification. Second, the same external measurements and AFCs (by ultrasound) were analyzed in live Tabapuã (Zebu) cows (n = 48). Relative RL and vulvar width were defined as the original measurements divided by the body weight of each cow for statistical analyses. In abattoir tracts, the AFC was smaller (P < 0.05) in animals with large VW (34.88 ± 3.50) than in animals with small (45.71 ± 2.57) and intermediate (42.25 ± 2.45) VW. The CQI was higher (P < 0.05) when the pelvic fat score was intermediate (3.22 ± 0.06) than that in lean (2.99 ± 0.08) and obese (2.90 ± 0.12) animals. The CQI was higher (P < 0.05) in the intermediate ovary group (3.19 ± 0.08) than in the small (2.96 ± 0.08) and large (2.95 ± 0.09) ovary groups. In live cows, the AFC was higher (P = 0.035) in females with large relative rima length than in those with small relative rima length (18.96 ± 1.97 and 14.76 ± 1.51, respectively). Similarly, AFC was greater (P = 0.0001) in females with large relative VW than in those with small relative VW (20.08 ± 1.36 and 11.16 ± 1.60, respectively). In conclusion, larger external genitalia relative to body size were good predictors of the ovarian follicular reserve in live animals.

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Section: Resultscontrasting

confidence: 45%

Vulvar width and rima length as predictors of the ovarian follicular reserve in bovine females

Mesquita

Maculan

Maciel

et al. 2016

Journal of Reproduction and Development

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“…injection of estradiol benzoate (EB) (0.25 mL Estrogin; Farmavet Produtos Veterinários Ltda, Brazil) [33,34] on the same day as the device was inserted (to induce atresia in ovarian follicles) [20], 700UI i.m. injection of eCG (3.5 mL Folligon 1000; Intervet International BV; Holland) on day 4 following device insertion (to induce superovulation, SOV) and 265 μg i.m.…”

Section: Methodsmentioning

confidence: 99%

Comparing two different superovulation protocols on ovarian activity and fecal glucocorticoid levels in the brown brocket deer (Mazama gouazoubira)

Zanetti¹,

Munerato²,

Cursino³

et al. 2014

Reprod Biol Endocrinol

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BackgroundStress is a limiting factor in assisted reproduction in wild animals maintained in captivity. However, the knowledge of assisted reproduction techniques for wild animals is useful for future in situ and ex situ conservation programs. Thus, this study evaluated the ovulation rate, presence of functional corpora lutea and fecal glucocorticoid levels following treatments promoting superovulation in captive brown brocket deer.MethodsThe crossover design used six hinds, allocated to two groups (n = 6): eCG Treatment, CIDR for 8 days, followed by 0.25 mg of EB on day 0, 700 IU of eCG on day 4 following device insertion and 265 mug of PGF2alfa on day 8; and FSH Treatment, CIDR for 7.5 days, followed by 0.25 mg of EB on day 0, 130 mg of FSH in 8 equal doses and 265 mug of PGF2alfa on day 7.5. Induced adrenal activity and treatment efficacy were evaluated by corpora lutea (CL) counts and fecal glucocorticoid and progestin concentration (ng/g feces) analyses for five different phases: Pre, two days before treatment; Early, first four days of treatment; Late, last four days of treatment; Total, entire treatment period; and Post, five days posttreatment.ResultseCG Treatment resulted in the highest number of CL (P lower than 0.05). There was no significant difference for fecal glucocorticoid concentrations in five different time periods between the treatments; however Pre fecal glucocorticoid concentrations (90.06+/−19.64) were significantly different from Late (200.76+/−26.39) within FSH Treatment. The mean fecal progestin concentration and mean ovulation rate were higher in eCG Treatment (4293.69+/−769.47, 7.0+/−1.8) than in FSH Treatment (1571.26+/−240.28, 2.6+/−0.8) (P lower than or equal to 0.05).ConclusionsAlthough the eCG Treatment induced a good superovulatory response, with the formation of functional corpora lutea, we cannot yet affirm that we have established a suitable protocol for induction of SOV in the species M. gouazoubira because approximately 65% of the deer showed premature regression of the corpora lutea. Moreover, multiple FSH applications in FSH Treatment resulted in a low ovulation rate and induced an increase in fecal glucocorticoid levels.

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“…Each implant released 20 mg of deslorelin per 24 h. Seven days after treatment at random stages of the estrous cycle, superstimulatory FSH treatments were initiated and 2 days later PGF2 was administered; 60 h after the PGF2 treatment, ovulation was induced with an injection of pLH (D'Occhio et al, 1997). This treatment protocol was compared with the EB-CIDR superstimulation protocol in Nelore cows and the number of transferable embryos did not differ (Barros and Nogueira, 2001). Unfortunately, deslorelin implants are not commercially available for use in cattle.…”

Section: Superovulation In Cattlementioning

confidence: 99%

Relative Roles of FSH and LH in Stimulation of Effective Follicular Responses in Cattle

Crowe¹,

Mullen²

2013

Gonadotropin

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Embryo transfer in cattle

Cited by 60 publications

References 76 publications

Vulvar width and rima length as predictors of the ovarian follicular reserve in bovine females

Vulvar width and rima length as predictors of the ovarian follicular reserve in bovine females

Comparing two different superovulation protocols on ovarian activity and fecal glucocorticoid levels in the brown brocket deer (Mazama gouazoubira)

Relative Roles of FSH and LH in Stimulation of Effective Follicular Responses in Cattle

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