Embryo Transfer in Cattle Production and Its Principle and Applications

Mebratu, Biruh

doi:10.18782/2394-3726.1083

Cited by 10 publications

(11 citation statements)

References 14 publications

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“…However, this study pointed out that the embryo transfer technique has yet to significantly succeed in Pakistan. Mebratu, Fesseha & Goa (2020) highlighted that the non-availability of the technical staff, the high cost involved in IVF/MOET, and the lack of fixed timeline-based superovulation protocols in subtropical conditions are the underlying factors for ET’s low adaptability in developing countries.…”

Section: Introductionmentioning

confidence: 99%

GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

Mahmood

Tahir

Butt

et al. 2021

PeerJ

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Multiple Ovulation and Embryo Transfer (MOET) technology is a potential technique to upgrade livestock species’ genetics. The varied response to super-stimulatory treatments remains one of the limiting factors to this technology’s widespread use. The present study was aimed to improve the superovulation response and in-vivo embryo production by using controlled internal drug release (CIDR)-GnRH or CIDR-EB (Estradiol Benzoate) along with conventional superovulation protocol in Holstein Frisian (HF): Bos taurus; n = 42) and Crossbred (XB: Cholistani (Bos indicus) × HF; n = 28) cows. In the CIDR-GnRH/CIDR-EB treatment, CIDR was implanted in the cows after confirming the presence of a corpus luteum (CL) on the 8th day after estrus. 2 ml GnRH (Lecirelin acetate 0.0262 mg/ml) or 2 mg EB was also administered in CIDR-GnRH/CIDR-EB groups, respectively. Both groups were given super-stimulatory treatment from the 11th day after estrus (FSH in tapering doses twice a day for four consecutive days). On day 13, two doses of 2 ml prostaglandin (75 µg/ml of dextrorotatory cloprostenol) were administered (am: pm), and CIDR was removed the following day. Two artificial inseminations (AI) of the cows were performed (12 h apart) on the 15th day. No CIDR and GnRH/E.B were given in the control group, but the remaining superovulation protocol was the same. Later on, seven days after the first AI, non-surgical embryo flushing was done. The transferable embryos produced from three different superovulation protocols were then transferred into the recipient cows (n = 90) for determining their fertility. Statistical analysis revealed that the number of super-estrus follicles (SEF), multiple corpora lutea (MCL), ovulation/fertilization percentage, fertilized structures recovered (FSR), and transferable embryos (TEs) remained significantly higher (p < 0.05), and days taken for return to estrus (RTE) after embryo collection remained significantly lower (p < 0.05) in CIDR-GnRH (n = 18) and CIDR-EB (n = 15) groups as compared to the control (n = 37). The comparison between XB and HF cows revealed that the TEs production in CIDR-GnRH (XB = 5 vs HF = 13) and CIDR-EB (XB = 6 vs HF = 9) based superovulation protocols were 11.60 ± 4.08 vs 04.31 ± 0.98 and 09.33 ± 1.78 vs 05.22 ± 1.36, respectively. TEs production in XB cows (n = 5) of the CIDR-GnRH group was significantly higher (11.60 ± 4.08) than other groups. On the other hand, the days taken for RTE after embryo collection remained significantly lower (p < 0.05) in HF cows of treatment groups. However, the fertility of TEs was neither affected significantly (p > 0.05) by the superovulation protocol used nor by breed differences among donor cows. In conclusion, using CIDR-GnRH or CIDR-EB along with conventional superovulation protocol may enhance the efficiency of MOET programs in cattle. Furthermore, XB donor cows demonstrated a better performance than HF donor cows under subtropical conditions.

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Section: Introductionmentioning

confidence: 99%

GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

Mahmood

Tahir

Butt

et al. 2021

PeerJ

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“…As the world's population has increased, the demand for animal products has increased several times. Superovulation and embryo transfer techniques have become important methods in cattle breeding (32)(33)(34). Studies have found that one injection of inhibin antigen is equivalent to multiple injections of exogenous gonadotropin in inducing the superovulation response of cattle (7).…”

Section: Discussionmentioning

confidence: 99%

Immunization Against Inhibin Promotes Fertility in Cattle: A Meta-Analysis and Quality Assessment

et al. 2021

Front. Vet. Sci.

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Superovulation and embryo transfer techniques are important methods in cattle breeding. Combined with traditional superovulation protocols, immunization against inhibin can further improve follicular development and embryo yield. The aim of this study is to determine the efficacy of immunization against inhibin in improving the fertility of cattle through meta-analysis and to provide better clinical veterinary practice guidance. Three English databases (PubMed, EMBASE, Web of Science) were searched for research articles of immunizations against inhibin influence on cattle fertility. Literature screening, data extraction, and meta-analysis were conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. In addition, the Systematic Review Center for Laboratory animal Experimentation (SYRCLE) risk-of-bias (RoB) tool was used to assess the risk of bias of the included animal studies. Potentially relevant studies (317) were identified, and finally 14 eligible studies (all in English) were included. The results of meta-analysis revealed that immunization against inhibin has significant effects on improving the number of ovulations [mean difference (MD) = 0.44, 95% confidence interval (CI) = (0.31, 0.56)], embryos and unfertilized ova [MD = 4.51, 95% CI = (2.28, 6.74)], follicles of the three size categories, the incidence of multiple ovulations [OR = 22.50, 95% CI = (8.13, 62.27)], and the conception rate [OR = 2.36, 95% CI = (1.26, 4.40)]. Moreover, it improved the production of embryos [grades 1 embryos: MD = 3.84, (3.54, 4.15); grade 2 embryos: MD = −0.73, (−0.89, −0.57); grade 3 embryos: MD = −0.50, (−0.75, −0.25); degenerated embryos: MD = 1.16, (−0.51, 2.82); transferable embryos: MD = 2.67, (2.03, 3.31)] and the number of corpora lutea [MD = 1.25, 95% CI = (0.79, 1.71)]. In the above indicators, the differences between the two groups were statistically significant (all p < 0.0001). Additionally, according to the quality evaluation results, the risk of bias in the included studies is relatively high. The quality evaluation of the results of the included studies showed that the risk of bias mainly concentrated in the selective, performance, detection, and reporting of bias aspects.

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“…The stages of in vivo embryo production include the selection of donor and recipient animals, the synchronization of the selected donors and recipients' oestrous cycles, the induction of superovulation and subsequent insemination of the donors, the collection (via uterine flushing), evaluation and transfer of embryos (Kaymaz 2012;Phillips and Jahnke 2016). Donors, fewer than five calving, are selected among normal cyclic animals free from hereditary diseases, reproductive disorders and diseases that may adversely affect fertility and have a high genetic potential and high production yields (Mapletoft and Bo 2006;Kidie 2019;Mebratu et al 2020). Donors should be animals of superior genetic merit that are capable of producing a high number of useable embryos (Genzebu 2015;Kidie 2019).…”

Section: Introductionmentioning

confidence: 99%

Embryo Transfer in Fleckvieh Simmental Cows: A Preliminary Study

2022

Int J Vet Sci

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This study was aimed at investigating pregnancy rates achieved with multiple ovulation and embryo transfer in Simmental cows, which are characterized by a high milk yield. For this purpose, eight cows were used as donors and for each donor, recipient groups of 10 animals, including both heifers and cows, were established. Recipients were synchronized by two intramuscular injections of prostaglandin F2α 11 days apart. The treatment of the donors consisted of the intravaginal placing of a CIDR on day 0, the application of 400mg FSH in decreasing doses, starting on day four, the administration of prostaglandin F2α and the removal of CIDR on day 8. The donors were inseminated twice on day 9 and once on day 10. Uterine flushing was performed, trans-cervically, on day 7 after insemination. The ovulation rate was determined as 12.25±1.42. Sixteen transferable embryos (2.0±3.23, 88.9%) were collected. The 12 embryos of Grade 1 quality and the 4 embryos of Grade 2 quality were transferred by transcervical route to 12 heifers and 4 cows, respectively. The pregnancy rates achieved in the heifers and cows were 50% and 25%, respectively (P>0.05). As a result, the MOET scheme applied in Fleckvieh Simmental Cows produced satisfactory superovulatory responses and resulted in the collection of quality embryos. The embryo recovery rate was poor but, overall, the ovulation rate, the quality of the collected embryos and the pregnancy rates achieved under field conditions in this preliminary study were considered to be satisfactory.

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Embryo Transfer in Cattle Production and Its Principle and Applications

Cited by 10 publications

References 14 publications

GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

Immunization Against Inhibin Promotes Fertility in Cattle: A Meta-Analysis and Quality Assessment

Embryo Transfer in Fleckvieh Simmental Cows: A Preliminary Study

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