Embryo production in superovulated Angus cows inseminated four times with sexed-sorted or conventional, frozen-thawed semen

Larson, J. E.; Lamb, G. C.; Funnell, Bethany J.; Bird, Scott; Martins, Aline Maria Araújo; Rodgers, Jean

doi:10.1016/j.theriogenology.2009.11.009

Cited by 30 publications

(30 citation statements)

References 17 publications

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“…The current result was consistent with some reports in which the proportion of high-quality embryos is inversely associated with the responsiveness of cattle for superovulation (20,22). When comparing with other studies conducted with different FSH protocols, transferable embryo proportions in present study were lower than in breeds of Sistani (49%), Angus (52%), Finnish Ayrhire (61%), Holstein (54%), Korean native (62%) and Japanese Black (53%), (6,26,31,34,37,41). However, similar results were reported in Nelore cows (1, 7).…”

Section: Discussionsupporting

confidence: 92%

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The effect of single epidural plus intramusculer injection of FSH on superovulatory response in Anatolian Black cow

SATILMIŞ¹

2012

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The present study was designed to determine whether a single epidural injection plus intramuscular (im) injection of FSH in order to reduce the number of injection treatments required to induce supurovulatory response, as well as to determine the effects of this treatment on Anatolian Black cow (Bos Taurus). The results will be compared to those obtained by administration of twice daily injections of FSH. Twenty-one Anatolian Black cows were used in this study. Anatolian Black cows received Cue-mate containing 1.56 g progesterone. The cows were divided into three groups (D1, D2 and E) based on the dose and administration route of FSH. In group D1 a total of 490 IU FSH and in group D2 a total of 700 IU FSH was given intramuscularly in decreasing doses for twice daily for 4 days. In both groups superovulatory treatments were initiated on day 7 of the cycle. In group E, FSH was given 175 IU epidural plus 175 IU im injection on day 7 of the cycle. On the 9th day of the cycle, the Cue-mate was removed and the cows received 500 µg prostaglandinF2α in all goups. The cows were artificially inseminated (AI) using semen obtained from native Anatolian Black bulls twice; in the evening of day 11 and in the morning of day 12 with 12 hours intervals. Superovulated cows were non-surgically flushed 7 days after AI. Embryos were morphologically evaluated and classified according to the criteria recommended by the IETS. No significant differences were observed in the mean number of Corpus luteum (CL) among groups which were 3.7 ± 0.56 in D1; 3.4 ± 0.55 in D2 and 6.0 ± 1.14 in E . There was no difference in transferable embryo yield among groups (10/42, 23.8% in D1; 14/32, 43.8% in D2 and 19/82, 23.2% in E). Untransferable embryo yield was not also significantly different among the groups (32/42, 76.2% in D1; 18/32, 56.2% in D2 and 63/82, 76.8% in E). The UFO rate was similar in all groups (17/77, 22.1% in D1; 13/71, 18.3% in D2 and 28/132, 21.2% in E). Recovery rate was found to be significant among groups (59/77, 76.6% in D1; 45/71, 63.4% in D2 and 110/132, 83.3% in E). To conclude, a single epidural injection plus an im injection of FSH application can be obtained acceptable results compared to twice daily injection of FSH for superstimulatory response. However transferable embryo rate of Anatolian Black cow was found to be low in both single epidural injection plus im injection and twice daily FSH applications. Further studies are required to obtain more concrete results on increasing transferable embryo yields in different breeds.Key words: Cow, embryo, epidural injection, FSH, superovulation.İntramuskuler uygulama ile birlikte epidural boşluğa uygulanan FSH'nın Yerli Kara inekte süperovulasyona olan etkisi Özet: Bu çalışma süperovulasyon oluşturmak için tekrarlanan dozlarda yapılan FSH uygulama sayısını azaltmak, FSH'nın tek uygulama şeklinde epidural-im olarak uygulanmasının süperovulasyon oluşturma yönünde etkisini belirlemek ve bu uygulamanın Yerli Kara inekte etkisini ortaya koymak amacıyla tasarlandı. Elde edile...

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Section: Discussionsupporting

confidence: 92%

“…Unfertilized oocytes (UFO) rates were similar in all groups. In this study, UFO rate was found higher than some previous studies which ranged from 23.5% to 39% in different breeds (26,31,38,41). This may due to asynchronous ovulation and fertilization failure.…”

Section: Discussioncontrasting

confidence: 68%

The effect of single epidural plus intramusculer injection of FSH on superovulatory response in Anatolian Black cow

SATILMIŞ¹

2012

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…Similar results have been reported previously (Kaimio et al, 2013). These results were observed even when greater sperm concentrations (10 x 10 6 and 20 x 10 6 ) were used in heat detection AI (Sartori et al, 2004), when timed AI (TAI) was used in heifers that were superovulated without an ovulation inductor (Schenk et al, 2006), when AI was conducted 6 h after the pre-established time (Soares et al, 2011), and when four inseminations were done with estrus observation (Larson et al, 2010).…”

Section: Discussionsupporting

confidence: 77%

“…Although the sperm sex-sorting process has significantly evolved, when it is used in superovulated cows the fertilization rate is lower than when using nonsorted semen (Sartori et al, 2004;Larson et al, 2007Larson et al, , 2010Soares et al, 2011;Kaimio et al, 2013). This result can be explained by the low sperm concentration and damage caused by the sorting process followed by freezing (dilution, laser exposure, process speed and centrifugation) (Schenk et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have reported the use of different strategies for increasing the results of artificial insemination (AI) using sex-sorted semen in superovulated donors. These strategies include using different sperm concentrations (Sartori et al, 2004;Schenk et al, 2006), using fresh sex-sorted semen (Hayakawa et al, 2009), using a higher number of AIs (Peippo et al, 2009;Larson et al, 2010) and using AI at different times (Soares et al, 2011). The use of sexsorted semen in superovulated cattle is promising when considering the limitations of in vitro embryo production related to cryopreservation (Viana and Camargo, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Fertilization rate and embryo production of superovulated dairy cows after insemination with non-sorted and sex-sorted semen

et al. 2016

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The aim of this study was to evaluate the fertilization rate of cows that were superovulated and artificially inseminated with sex-sorted semen. Cows were treated with an intravaginal progesterone device plus estradiol benzoate (day 0). Superstimulation treatments began four days after with eight applications of FSH at 12 h intervals. D-Cloprostenol was administered on day 6. Progesterone device was removed on day 7, and LH was administered on day 8. The treatments were divided as follows: NonSx, two AI with non-sorted semen were conducted 12 and 24 h after LH; Sx12&24, two AI with sex-sorted semen were conducted 12 and 24 h after LH; and Sx24&36, two AI with sex-sorted semen were conducted 24 and 36 h after LH. Embryos were recovered on day 16 and were evaluated and classified. Percentage of fertilized embryos tended to be greater for the non-sorted semen than the sex-sorted semen. The number of unfertilized oocytes was smaller when the non-sorted semen was used relative to the sex-sorted semen. There was no difference between the treatments that used sexed semen. In conclusion, the use of sex-sorted semen in superovulated dairy cows results in greater numbers of unfertilized oocytes than non-sorted. However, when only sorted semen is used AI should be performed 24 and 36 h after LH.

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Utilization of Sex‐sorted Semen

Kasimanickam

2014

Bovine Reproduction

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Embryo production in superovulated Angus cows inseminated four times with sexed-sorted or conventional, frozen-thawed semen

Cited by 30 publications

References 17 publications

The effect of single epidural plus intramusculer injection of FSH on superovulatory response in Anatolian Black cow

The effect of single epidural plus intramusculer injection of FSH on superovulatory response in Anatolian Black cow

Fertilization rate and embryo production of superovulated dairy cows after insemination with non-sorted and sex-sorted semen

Utilization of Sex‐sorted Semen

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