Embryo production in heifers with low or high dry matter intake submitted to superovulation

Mollo, Marcos Rollemberg; Monteiro, P. L. J.; Surjus, R. S.; Martins, Augusto; Ramos, A. F.; Mourão, Gérson Barreto; Carrijo, L. H. D.; Lopes, G.; Rumpf, R.; Wiltbank, Milo C.; Sartori, Roberto

doi:10.1016/j.theriogenology.2017.01.015

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…The 100% and 180% maintenance feeding groups showed differences in the total number of embryos (14.1 vs. 9.5, p < 0.05) and number of viable embryos (10.1 vs. 6.7, p < 0.05) [ 28 ]. The 70% and 170% maintenance feeding groups showed differences in the number of viable (5.3 vs. 3.8, p = 0.028) and total number of embryos (9.9 vs. 6.7, p = 0.001) [ 29 ]. Previous studies demonstrated that low-diet feeding to cows is suitable for producing high quality and transferable embryos.…”

Section: Discussionmentioning

confidence: 99%

Nutrient requirements in Hanwoo cows with artificial insemination: effects on blood metabolites and embryo recovery rate

Kang¹,

Kim²,

Han³

et al. 2020

J Anim Sci Technol

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Here, we investigated the effects of different nutrient requirements (NR) on blood metabolites, transferable embryo number after multiple superovulations with artificial insemination (AI), body condition score (BCS), and estrus cycle in Hanwoo cow. Nineteen Hanwoo cows were randomly divided into three groups (80%, 100%, and 120% NR, containing 6, 8, and 5 individuals, respectively) and fed based on the NR. In experiment 1, glucose, total cholesterol, triglyceride, blood urea nitrogen (BUN), aspartate aminotransferase (AST), alanine aminotransferase (ALT), non-esterified fatty acids (NEFA), albumin (ALB), and total protein (TP) were analyzed. In experiment 2, total number of recovered embryos and transferable embryos was examined after embryo recovery and multiple superovulations with AI. In experiment 3, body weight, BCS, and estrus cycle were examined. In experiment 1, total cholesterol was significantly different among the 80%, 100%, and 120% NR groups (126.5 ± 3.3, 152.6 ± 2.4, and 177.4 ± 1.8 mg/dL, respectively, p < 0.05). The triglyceride and BUN levels in the 120% NR group were significantly higher than those in the 80% and 100% groups ( p < 0.05). The NEFA levels were significantly different among the 80%, 100%, and 120% NR groups (440.5 ± 18.2, 318.5 ± 23.1, and 195.1 ± 8.5 ЧEq/L, respectively, p < 0.05). The AST and TP levels in the 80% NR group were significantly lower than those in the 100% and 120% NR groups ( p < 0.05). In experiment 2, the 120% NR group showed a higher percentage of transferable embryos than the 80% and 100% groups ( p < 0.01). The mean body weight and BCS among the 80%, 100%, and 120% NR groups were significantly different ( p < 0.05). The estrus cycle in the 80% NR group was delayed compared with the 100% and 120% NR groups (20.8 ± 0.2 and 21.2 ± 0.5 days, respectively). In conclusion, the blood metabolic tests proved that Hanwoo cows with 120% NR can produce a large number of transferable embryos. Thus, 120% NR is the appropriate feeding level for this type of cows as it results in the production of a large number of transferable embryos by multiple superovulations with AI.

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Section: Discussionmentioning

confidence: 99%

Nutrient requirements in Hanwoo cows with artificial insemination: effects on blood metabolites and embryo recovery rate

Kang¹,

Kim²,

Han³

et al. 2020

J Anim Sci Technol

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“…The present work evaluated multiparous, non-lactating and non-pregnant Nellore cows (beef cattle), whose DMI was obtained daily and individually for a long period (67 days). Other studies aiming to understand the effects of nutritional management and metabolism on reproductive efficiency also validated the experimental model in beef cattle with different DMI-inducing changes in BER [ 6 , 23 , 42 ]. In ruminants, the glucose is obtained by ruminal volatile fatty acids and, despite these animals having a low peripheral glucose concentration, glucose acts as the main substrate for cell metabolism [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…Cow nutrition influences ovarian follicles’ selection and development, and changing body energy condition may alter the follicular environment and gametic/embryonic quality [ 7 , 51 ]. The increase in BER by the high DMI promotes lower response to the multiple ovulation protocol, subsequently lower ovulation rates [ 23 , 52 ] and increased propensity of ovarian cysts [ 52 ]. However, it is important to consider that the ovarian response and embryo quality is dependent on the animal’s initial BER.…”

Section: Discussionmentioning

confidence: 99%

“…The EVs within the follicular fluid, are one of the main communication agents, actively interact with granulosa cells and CCs, sending bioactive molecules and, thus, assisting in the oocyte’s process of acquiring competence [ 13 , 15 , 59 ]. In high BER animals, the elevated DMI appears to affect later follicular developmental stages as well as early embryonic development [ 23 , 60 ], suggesting an influence in communication between follicular compartments as early as 3–6 mm follicular diameter. Based on our data, the functional enrichment analysis for miRNAs differentially expressed between CC and EV FF in each group, we found predicted pathways related to cell proliferation, survival, growth and metabolism, which are biological processes associated with the follicular development.…”

Section: Discussionmentioning

confidence: 99%

“…Although different studies have described the diet effects [ 22 ], nutritional management [ 23 , 24 ] and metabolism [ 6 , 25 ] on ovarian response, oocyte and embryonic quality, little is known about these reproductive responses after increasing BER in beef cows. Therefore, the hypothesis for this work is that the increase of BER in Nellore cows negatively influences ovulation and embryo recovery rates by changes in the miRNA profile of extracellular vesicles present within the follicular environment.…”

Section: Introductionmentioning

confidence: 99%

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High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle

et al. 2023

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Aiming to evaluate the effects of increased body energy reserve (BER) in Nellore cows’ reproductive efficiency, cows were fed with different nutritional plans to obtain animals with high BER (HBER; Ad libitum diet) and moderate BER (MBER: cows fed 70% of HBER group ingestion). To evaluate the BER, cows were weekly weighted and evaluated for subcutaneous fat thickness and insulin serum concentration along the experimental period. At the end of the experimental period, animals were submitted to estrous synchronization and artificial insemination. Animals were slaughtered approximately 120 h after ovulation induction and the reproductive tracts were collected for embryo recovery and samples collection. Cumulus-oocyte-complexes (COC) and follicular fluid were collected from 3–6 mm in diameter ovarian follicles to perform miRNA analysis of cumulus cells (CC) and extracellular vesicles from follicular fluid (EV FF). As expected, differences were observed among MBER and HBER groups for body weight, fat thickness, and insulin serum concentration. HBER animals showed lower ovulation and embryo recovery rates compared to MBER animals. Different miRNAs were found among CC and EV FF within groups, suggesting that the BER may influence follicular communication. This suggests that small follicles (3–6 mm diameter) are already under BER effects, which may be greater on later stages of follicular development.

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Early resynchronization of follicular wave emergence among Nelore cattle using injectable and intravaginal progesterone for three timed artificial inseminations

Junior

Kloster

Moraes

et al. 2021

Animal Reproduction Science

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Embryo production in heifers with low or high dry matter intake submitted to superovulation

Cited by 6 publications

References 28 publications

Nutrient requirements in Hanwoo cows with artificial insemination: effects on blood metabolites and embryo recovery rate

Nutrient requirements in Hanwoo cows with artificial insemination: effects on blood metabolites and embryo recovery rate

High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle

Early resynchronization of follicular wave emergence among Nelore cattle using injectable and intravaginal progesterone for three timed artificial inseminations

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