Embryo production from superovulated Holstein-Friesian dairy heifers and cows after insemination with frozen-thawed sex-sorted X spermatozoa or unsorted semen

Peippo, Jaana; Vartia, K.; Kananen-Anttila, K.; Räty, Mervi; Korhonen, Kati; Hurme, Timo; Myllymäki, Hannu; Sairanen, Auvo; Mäki‐Tanila, Asko

doi:10.1016/j.anireprosci.2008.02.002

Cited by 51 publications

(41 citation statements)

References 15 publications

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“…In fact, the number of transferable embryos in rbST-I and -II treatments was lower than those reported by Kuehner et al (1993) and Herrier et al (1994) in which only one injection of rbST was administered. The number of transferable embryos produced by donor heifers in our study was far from what we expected from the superstimulation treatment and much lower than that found in other studies without rbST administration and using sexed semen (Sartori et al, 2004;Hayakawa et al, 2009;Peippo et al, 2009). The lack of recovery of structures from a large proportion of donor heifers receiving two injections of rbST might have contributed to the low values for production of transferable embryos.…”

Section: Embryo Yield Of Donors Treated With Rbstcontrasting

confidence: 53%

See 1 more Smart Citation

Superovulatory response and embryo quality of Holstein heifers treated with one or two injections of somatotropin

González-Maldonado

Santos

Lara

2015

Rev Colomb Cienc Pecu

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Section: Embryo Yield Of Donors Treated With Rbstcontrasting

confidence: 53%

“…Sartori et al (2004) and Peippo et al (2009) published that in 13 to 25% of the donors only one or none structures was collected. Nonetheless, these earlier results are lower than those obtained in our study.…”

Section: Embryo Yield Of Donors Treated With Rbstmentioning

confidence: 99%

Superovulatory response and embryo quality of Holstein heifers treated with one or two injections of somatotropin

González-Maldonado

Santos

Lara

2015

Rev Colomb Cienc Pecu

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“…Comparing the efficiency of embryo sexing versus sperm sexing method, recent publications report worse results with sexed sperm compared to unsexed sperm, when X chromosome-bearing fractions of sorted sperm were used for IVF (Blondin et al, 2009), for insemination (Schenk et al, 2009) and in multiple ovulation and embryo transfer (MOET) programs (Hayakawa et al, 2009;Peippo et al, 2009). The use of low-dose X-sorted spermatozoa for the insemination of superovulated embryo donors can improve the proportion of transferable female embryos produced but this potential may be not realised in commercial bovine practice, due to reduced fertilization rates using X-sorted spermatozoa (Peippo et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The use of low-dose X-sorted spermatozoa for the insemination of superovulated embryo donors can improve the proportion of transferable female embryos produced but this potential may be not realised in commercial bovine practice, due to reduced fertilization rates using X-sorted spermatozoa (Peippo et al, 2009). The lower effectiveness may be due to procedures associated with sorting, handling of sperm, bull selection, instrumentation, mode of insemination, management level, etc.…”

Section: Discussionmentioning

confidence: 99%

Conception rate after sex determination and cryopreservation of D7 bovine embryos:

Lopatářová¹,

Čech²,

Krontorád³

et al. 2010

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to evaluate conception rate after sex determination and subsequent freezing of Day 7 (D 7 ) bovine embryos and to compare it with transfer of fresh female embryos. High quality embryos obtained from superovulated donors were biopsied and the embryonic cells (5-10) were analysed by PCR using specific primers for the Y-chromosome determinant. Fresh embryos were blade biopsied (n = 172) and subsequently transferred (ET) ipsilaterally to synchronized recipients. Selected embryos (n = 112) were biopsied by cell aspiration and cryopreserved for later transfer. Sex determination was successfully completed in 91.3% of fresh group embryos (44% female) and in 87.5% of freeze-thawed group embryos (45.9% female). The achieved pregnancy rate was 56.5% after fresh transfer of sexed (female) embryos whereas intact embryos (control 1) implanted in 61.9% recipients. 95.6% (43/45) of embryos survived biopsy and cryopreservation. After transfer of freeze-thawed sexed (female) embryos, pregnancy was established in 48.8% of recipients. A similar conception rate was found in intact embryos (50.7%, control 2). The results clearly demonstrate that the microsurgical technique used and subsequent PCR sex analysis allow the rapid commercial exchange of genetic resources on the basis of fresh or frozen sex-desired embryos in embryo transfer programs.

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“…La presente investigación consideró únicamente como donadoras a vaquillas, porque estas muestran mayor porcentaje de embriones viables que las vacas lactantes y no lactantes superovuladas (Peippo et al, 2009;Sartori et al, 2002 Recuperación, evaluación y clasificación de embriones La recuperación de embriones se realizó mediante un método no quirúrgico a los siete días después de la primera IA (día 15 del Protocolo, en la mañana). Previamente, se aplicó anestesia epidural a la donante, y luego se introdujo y se fijó un catéter armado a partir de una sonda Foley en la curvatura mayor de cada cuerno uterino.…”

Section: Selección Y Superovulación De Hembras Donadorasunclassified

Evaluación de la Respuesta Superovulatoria en vaquillas Simmental del núcleo genético de la Universidad Nacional Toribio Rodríguez de Mendoza, Amazonas

Murga

Gonzales

Bardales

2017

RICBA

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<p>El objetivo del presente estudio fue evaluar la eficiencia de un protocolo de superovulación en vaquillas de raza Simmental de la Universidad Nacional Toribio Rodríguez de Mendoza. Se realizaron 58 superovulaciones a once vaquillas en base al protocolo reportado por Bó <em>et al. </em>(2006). Se colectaron embriones con el método no quirúrgico y se clasificaron de acuerdo a la IETS (Sociedad Internacional de Transferencia de embriones). El número total de embriones obtenido fue 247 de los cuales 80,16 % fueron mórulas; el 12,55 %, blastocistos temprano; el 5,67 %, blastocistos y el 1,62 %, blastocistos expandidos. Se obtuvo, en promedio, 7,18 ± 4,33 estructuras, 4,26 ± 3,56 embriones transferibles y 1,76 ± 1,99 embriones degenerados. Se encontró diferencia estadística, según el número de superovulaciones sucesivas sobre la producción de estructuras y embriones transferibles. En todos los criterios de evaluación del presente estudio, la respuesta superovulatoria respecto al número de estructuras y de embriones transferibles fueron las variables que se vieron significativamente afectadas. Esto permite concluir que las vaquillas de raza Simmental tuvieron una respuesta superovulatoria óptima al protocolo aplicado.</p>

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Embryo production from superovulated Holstein-Friesian dairy heifers and cows after insemination with frozen-thawed sex-sorted X spermatozoa or unsorted semen

Cited by 51 publications

References 15 publications

Superovulatory response and embryo quality of Holstein heifers treated with one or two injections of somatotropin

Superovulatory response and embryo quality of Holstein heifers treated with one or two injections of somatotropin

Conception rate after sex determination and cryopreservation of D7 bovine embryos:

Evaluación de la Respuesta Superovulatoria en vaquillas Simmental del núcleo genético de la Universidad Nacional Toribio Rodríguez de Mendoza, Amazonas

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