Embryo/larval toxicity and transcriptional effects in zebrafish (Danio rerio) exposed to endocrine active riverbed sediments

Viganò, Luigi; Casatta, Nadia; Farkas, Anna; Mascolo, G.; Roscioli, Claudio; Stefani, Fabrizio; Vitelli, Matteo; Olivo, F; Clerici, Laura; Robles, Pasquale; Dellavedova, Pierluisa

doi:10.1007/s11356-019-07417-8

Cited by 15 publications

(4 citation statements)

References 108 publications

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“…Several endpoints have been evaluated to disclose signals of lead toxicity in fish sentinel species, including zebrafish. Neurotoxicity, oxidative stress induction, and hematological alterations are the mainly reported outcomes of lead exposure in both adults and embryos [8,[22][23][24]29,30,34,[44][45][46][47]. However, all these literature data relate to high lead concentrations, thus leaving a gap of knowledge regarding the adverse effects of lead at environmentally relevant concentrations [9,53].…”

Section: Discussionmentioning

confidence: 99%

“…In fish, lead can be absorbed from water, food, or sediment, but regardless of the route of entry into the body shows a high tendency to bioaccumulation in various fish tissues (including gills, liver, skin, and muscles), depending on environmental factors, species-specific features, concentration, and time of exposure [8,19,20]. From literature, it is known that in fish, embryos and larvae are the most sensitive life stages, and they can therefore serve as an early indicator of pollution even at low levels of exposure, which also applies to Pb and other heavy metals [21][22][23]. Nevertheless, Pb-induced alterations during development are largely overlooked, and most of the available studies have been conducted on adults.…”

Section: Introductionmentioning

confidence: 99%

“…Considering that the combination of morphological changes and transcriptional responses is recognized as valid and promising tools for studying ecotoxicological effects [23], in a second step, we also applied molecular biomarkers to have a more comprehensive overview of Pb-induced outcomes. In detail, we evaluated the expression pattern of some genes involved in the early developmental process (Wnt and β-catenin) to address the mechanism underlying the putative morphological modifications caused by Pb exposure.…”

Section: Introductionmentioning

confidence: 99%

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Morphological and Molecular Alterations Induced by Lead in Embryos and Larvae of Danio rerio

et al. 2021

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Lead (Pb) is one of the most toxic and persistent elements and may adversely affect both humans and wildlife. Given the risks posed to humans, lead is listed among priority substances of public health importance worldwide. In fish, available studies deal with high doses, and the potential hazard of Pb at low concentrations is largely unknown. Given its well-demonstrated translational value for human toxicity research, we used zebrafish as a model species. Embryos were exposed to two environmentally relevant concentrations of lead (2.5 and 5 µg/L) from 6 h post-fertilization and analyzed after 48, 96, and 144 h. The morphological abnormality arose after 48 h, and the incidence and intensity were dose and time dependent. Spinal and tail deformities were the most frequently detected alterations. Pb also modulated the expression of genes involved in the toxicological responses (sod and mt), thus demonstrating that zebrafish’s early stages are able to mount an adaptive response. Moreover, ldh and β-catenin were significantly upregulated in all groups, whereas wnt3 expression was increased in the high concentration group. Our results confirm that zebrafish embryos and larvae are valuable early warning indicators of pollution and may play a major role in ecosystems and human health monitoring.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphological and Molecular Alterations Induced by Lead in Embryos and Larvae of Danio rerio

et al. 2021

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“…For these reasons and considering that the combination of morphological changes and transcriptional responses is recognized as valuable and promising tools for the study of ecotoxicological effects [ 33 ], in the present work, we first investigated changes in male gonadal morphology and the level of steroidogenic enzymes after exposure of M. galloprovincialis for 24 h to 1, 10, 100 pM HgCl 2 . In addition, we assessed both the expression levels of three stress genes and the PL protein-coding genes and determined the difference in the accessibility of sperm chromatin to micrococcal nuclease at these doses of exposure to HgCl 2 .…”

Section: Discussionmentioning

confidence: 99%

Morphological, Gene, and Hormonal Changes in Gonads and In-Creased Micrococcal Nuclease Accessibility of Sperm Chromatin Induced by Mercury

et al. 2022

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Mercury is one of the most dangerous environmental pollutants. In this work, we analysed the effects of exposure of Mytilus galloprovincialis to 1, 10 and 100 pM HgCl2 for 24 h on the gonadal morphology and on the expression level of three stress genes: mt10, hsp70 and πgst. In this tissue we also evaluated the level of steroidogenic enzymes 3β-HSD and 17β-HSD and the expression of PL protein genes. Finally, we determined difference in sperm chromatin accessibility to micrococcal nuclease. We found alterations in gonadal morphology especially after exposure to 10 and 100 pM HgCl2 and hypo-expression of the three stress genes, particularly for hsp70. Furthermore, decreased labelling with both 3β-HSD and 17β-HSD antibodies was observed following exposure to 1 and 10 pM HgCl2 and complete absence at 100 pM HgCl2 exposure. Gonads of mussels exposed to all HgCl2 doses showed decreased expression of PL protein genes especially for PLIII. Finally, micrococcal nuclease digestions showed that all doses of HgCl2 exposure resulted in increased sperm chromatin accessibility to this enzyme, indicative of improper sperm chromatin structure. All of these changes provide preliminary data of the potential toxicity of mercury on the reproductive health of this mussel.

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Exposure to Landfill Leachates Affects the Embryonic Development of Zebrafish, Danio rerio: A Case Study in Yucatan, Mexico

Escalante-Mañé

Hernández-Nuñéz

Méndez-Novelo

et al. 2021

Bull Environ Contam Toxicol

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Embryo/larval toxicity and transcriptional effects in zebrafish (Danio rerio) exposed to endocrine active riverbed sediments

Cited by 15 publications

References 108 publications

Morphological and Molecular Alterations Induced by Lead in Embryos and Larvae of Danio rerio

Morphological and Molecular Alterations Induced by Lead in Embryos and Larvae of Danio rerio

Morphological, Gene, and Hormonal Changes in Gonads and In-Creased Micrococcal Nuclease Accessibility of Sperm Chromatin Induced by Mercury

Exposure to Landfill Leachates Affects the Embryonic Development of Zebrafish, Danio rerio: A Case Study in Yucatan, Mexico

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