Embryo development after heterotopic transplantation of cryopreserved ovarian tissue

Oktay, Kutluk; Büyük, Erkan; Veeck, Lucinda L.; Zaninović, Nikica; Xu, Kangpu; Takeuchi, Takumi; Opsahl, Michael S.; Rosenwaks, Zev

doi:10.1016/s0140-6736(04)15728-0

Cited by 504 publications

(265 citation statements)

References 17 publications

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“…Similarly, analysis of tumors, TNM classification and tumor characteristics (receptors and proliferation index) did not suggest any correlation between an aggressive tumor and positive qPCR results in the ovary. Nevertheless, some teams allow transplantation of frozen-thawed ovarian tissue from patients with early-stage breast cancer [2,23,33,37]. However, while most breast cancer recurrences manifest within 10 years of the initial diagnosis, the disease can recur much later, emphasizing the need for long-term surveillance in clinical follow-up of these patients [7].…”

Section: Discussionmentioning

confidence: 99%

Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study

Luyckx

Durant

Camboni

et al. 2013

J Assist Reprod Genet

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Section: Discussionmentioning

confidence: 99%

Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study

Luyckx

Durant

Camboni

et al. 2013

J Assist Reprod Genet

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“…Oktay et al also demonstrated that the human subcutaneous ovarian transplantation can preserve endocrine function (6,21) and the fertility was demonstrated by developing a four-cell embryo (21). They then transferred the embryo into the uterus but with no further illustration.…”

Section: Discussionmentioning

confidence: 99%

Blastocyst Development After Cryopreservation and Subcutaneous Transplantation of Mouse Ovarian Tissue

Lee

Ho²,

Yu³

et al. 2005

J Assist Reprod Genet

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Purpose : To investigate follicle survival and developmental potential with IVF of cryopreserved, subcutaneously transplanted mouse ovarian tissue. Methods : Fresh and frozen mouse ovarian tissue was autologously transplanted into subcutaneous tissue. Two weeks after the transplantation, the morphology and histology of the fresh and frozen grafts were compared. Superovulation and IVF was performed to evaluate the fertility potential of the frozen ovarian graft. Results : Both fresh and frozen grafts of ovarian tissue survived in 14 of 16 mice (88%). Morphologically, both types of grafts resembled fresh ovarian tissue and contained follicles at all stages of folliculogenesis. A total of 73% of follicles in fresh grafts and 62% in frozen grafts survived after transplantation compared with fresh ovarian tissue. Sixteen ICR mice underwent superovulation. A total of 56 oocytes from antral follicles were recovered from the subcutaneously transplanted cryopreserved ovarian tissue. Fourteen (25%) oocytes were in metaphase II stage, 6 were fertilized by IVF, and 2 progressed to the blastocyst stage. Conclusions : Cryopreservation and subcutaneous transplantation of ovarian tissue provides a possible means of fertility preservation. The main loss of follicles occurred during grafting rather than during freezing and thawing.

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“…Successful fertilization and pregnancy with oocytes from subcutaneously transplanted fresh ovarian tissue have been reported in a primate [15]. In humans, a 4-cell embryo was produced from subcutaneously transplanted cryopreserved ovarian tissue, but it did not result in conception [16]. We have reported blastocyst development from cryopreserved, subcutaneously transplanted mouse ovarian tissue [17].…”

Section: Introductionmentioning

confidence: 85%

“…Cryopreserved ovarian tissues are subject to freezing, thawing, and ischemic injury throughout the transplantation procedure. Grafting to a heterotopic site also potentially exposes the tissue to higher pressure or lower temperature than normal ovaries [16,19,20]. Presumably, these injuries impair the quality of the oocyte and subsequent embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

Abnormally low expression of connexin 37 and connexin 43 in subcutaneously transplanted cryopreserved mouse ovarian tissue

Lee

et al. 2008

J Assist Reprod Genet

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Purpose To analyze the gap junction proteins connexin 37 (Cx37) and connexin 43 (Cx43) after subcutaneous transplantation of cryopreserved mouse ovarian tissue. Methods Expression of gap junction genes was assessed by immunohistochemistry and real-time polymerase chain reaction (PCR) in transplanted cryopreserved ovarian tissue compared with that of normal ovarian tissue. Apoptosis of ovarian cells was evaluated by using the terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphates nick end-labeling method.Results After subcutaneous transplantation, Cx37 and Cx43 mRNA and protein expression were significantly lower in cryopreserved than in normal ovarian tissue. Apoptosis was increased in granulosa cells from antral follicles of the cryopreserved tissue. Conclusion After cryopreservation and subcutaneous transplantation of ovarian tissue, proteins forming gap junctions between oocytes and granulosa cells are under-expressed compared with normal controls.

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Embryo development after heterotopic transplantation of cryopreserved ovarian tissue

Cited by 504 publications

References 17 publications

Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study

Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study

Blastocyst Development After Cryopreservation and Subcutaneous Transplantation of Mouse Ovarian Tissue

Abnormally low expression of connexin 37 and connexin 43 in subcutaneously transplanted cryopreserved mouse ovarian tissue

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