Embryo cryopreservation and in vitro culture of preimplantation embryos in Campbell's hamster (Phodopus campbelli)

Amstislavsky, S. Ya.; Brusentsev, E. Yu.; Kizilova, Elena A.; Igonina, T. N.; Абрамова, Т. О.; Рожкова, И. Н.

doi:10.1016/j.theriogenology.2014.12.013

Cited by 16 publications

(22 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of the present study are in agreement with our previous observations on Campbell's hamsters (Amstislavsky et al. ), suggesting that the mixture of permeating (EG) and non‐permeating (sucrose) cryoprotectants may be suitable for freezing embryos of Phodopus species.…”

Section: Discussionsupporting

confidence: 93%

“…) and in Campbell's hamsters (Amstislavsky et al. ). The advantage of R1ECM, confirmed in the present study, may be explained by the fact that, unlike HECM (Schini and Bavister ), this medium contains glucose, although at a low concentration (Miyoshi et al.…”

Section: Discussionmentioning

confidence: 99%

“…; Amstislavsky et al. ). Poor‐quality embryos were discarded; good‐quality embryos without major defects and with intact zonae pellucidae were either frozen as described below or used as non‐frozen controls.…”

Section: Methodsmentioning

confidence: 98%

“…Our previous report demonstrated that GM‐CSF (2 ng/ml) improved in vitro embryo development in Campbell's hamsters as well (Amstislavsky et al. ).…”

Section: Introductionmentioning

confidence: 94%

“…In our previous study, assisted reproductive technologies (ART) were successfully applied to the latter species (Amstislavsky et al. ).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Cryopreservation and In Vitro culture of Preimplantation Embryos in Djungarian Hamster (Phodopus sungorus)

Brusentsev

Abramova

Rozhkova

et al. 2015

Reprod Domestic Animals

View full text Add to dashboard Cite

Although embryo cryobanking was applied to Syrian golden and to Campbell's hamsters, no attempt has been made at freezing embryos in Djungarian hamsters. Four-cell stage embryos were flushed from the reproductive ducts of pregnant females before noon of the third-day post coitum and frozen in 0.25-ml straws according to standard procedures of slow cooling. A mixture of permeating (ethylene glycol) and non-permeating (sucrose) cryoprotectants was used. The thawing was performed by incubating at RT for 40 s followed by 40 s in a water bath at 30.0°C. Most (66.7%) of the non-frozen four-cell embryos developed up to the morula stage in rat one-cell embryo culture medium (R1ECM). The use of hamster embryo culture medium (HECM) yielded fewer morulas (18.2%) during the same 24-h period of culture. The rate of embryo's surviving the freezing-thawing procedures, as estimated by light microscopy, was 60.7-68.8%. After 24-h culturing in R1ECM, 64.7% of frozen-thawed four-cell embryos developed and all of them reached the morula stage. Supplementation of R1ECM with GM-CSF (2 ng/ml) improved the rate of Djungarian hamster frozen-thawed embryo development: 100% of the four-cell stage embryos developed, 50% of them achieved the morula stage, and 50% developed even further and reached the blastocyst stage within 24 h of culturing. This study reports the world's first successful transfer of frozen-thawed Djungarian hamster embryos yielding term pups. Taken together, the results of this study demonstrate the possibility of applying some key reproductive technologies, that is, embryo freezing/cryopreservation and in vitro culture, to Djungarian hamsters.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 98%

“…Our previous report demonstrated that GM‐CSF (2 ng/ml) improved in vitro embryo development in Campbell's hamsters as well (Amstislavsky et al. ).…”

Section: Introductionmentioning

confidence: 94%

“…In our previous study, assisted reproductive technologies (ART) were successfully applied to the latter species (Amstislavsky et al. ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations