Embryo aneuploidy screening for repeated implantation failure and unexplained recurrent miscarriage

Fındıklı, Necati; Kahraman, Semra; Saglam, Yaman; Beyazyurek, C.; Sertyel, S.; Karlikaya, G.; Karagozoglu, H.; Aygün, Banu

doi:10.1016/s1472-6483(10)62014-7

Cited by 36 publications

(16 citation statements)

References 115 publications

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“…The PB1 aneuploidy rate was 34.5% in the recurrent implantation failure group (group 3), below values reported in pre-implantation genetic diagnosis studies [7,28,37]. This study confirms that oocyte chromosomal abnormalities play a key role in certain implantation failures, but also confirms the etiologic diversity of recurrent implantation failure.…”

Section: Discussionsupporting

confidence: 73%

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Gamete cytogenetic study in couples with implantation failure: aneuploidy rate is increased in both couple members

Vialard

Hammoud

Molina-Gomès

et al. 2008

J Assist Reprod Genet

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Purpose Implantation failure is known to be associated with an increased risk of aneuploidy in embryos, a situation leading to a pre-implantation genetic screening, not allowed in different countries like France. Our aim was to evaluate the gamete aneuploidy incidence in this context, using first polar body and spermatozoa aneuploidy screening. Methods Three groups were considered: 11 couples with pregnancy obtained after IVF for female infertility (group 1); 20 couples with pregnancy obtained after IVF for male infertility (group 2); and 35 couples with implantation failure (group 3). In group 3, 28 couples treated by ICSI volunteered for first polar body analysis (PB1). Results Spermatozoa aneuploidy rate was increased in groups 2 (1.6%) and 3 (2.1%) in comparison to group 1 (0.6%). PB1 aneuploidy rate was 35.4% in group 3. Finally, eight couples (32%) had no particular chromosomal risk in gametes, 15/25 (60%) presented an increased spermatic (>2%) or oocyte (>1/3) aneuploidy rate, and 2/25 (8%) had both. Conclusion Those results confirm that implantation failure has a heterogeneous origin, that gamete chromosome abnormality rate is one of the major contributing factors, and that 1st Polar body and spermatozoa aneuploidy screening or pre-implantation genetics screening may be indicated for these couples.

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Section: Discussionsupporting

confidence: 73%

“…Previous studies separately reported spermatozoa [19,20], polar body [4] or embryo aneuploidy rate evaluations [9,27,28]. This study is the first to deal with both spermatozoa and oocyte aneuploidy rates in couples with recurrent implantation failure.…”

Section: Discussionmentioning

confidence: 93%

Gamete cytogenetic study in couples with implantation failure: aneuploidy rate is increased in both couple members

Vialard

Hammoud

Molina-Gomès

et al. 2008

J Assist Reprod Genet

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“…cause of early pregnancy loss is thought to lie in chromosomal abnormality and aneuploidy of cleavage stage embryos [31,32]. Therefore, imaging of the chromosome dynamics using H2B-mRFP1 as a probe may help to assess the chromosome integrity of embryos and, furthermore, to eliminate the chromosomally 'illfated' embryos from transfer in ART.…”

Section: Applicationsmentioning

confidence: 99%

Long-Term, Six-Dimensional Live-Cell Imaging for the Mouse Preimplantation Embryo That Does Not Affect Full-Term Development

Yamagata

Suetsugu

Wakayama

2009

Journal of Reproduction and Development

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Abstract. Mammalian preimplantation embryonic development is achieved by tightly coordinated regulation of a great variety of temporal and spatial changes. Therefore, it would be valuable to analyze these events three-dimensionally and dynamically. We have previously developed a live-cell imaging method based on the expression of fluorescent proteins, using mRNA injection and time-lapse florescence microscopy. However, with conventional fluorescent microscopy, three-dimensional images could not be obtained due to the thickness of the embryos and the optical problem in which 'out-of focus blur' cannot be eliminated. Moreover, as the repeated exposure of intense excitation light to the cell yields phototoxicity, long-term observation was detrimental to embryonic development. Here, we improved our imaging system to enable six-dimensional live-cell imaging of mouse preimplantation embryos (x, y and z axes, time-lapse, multicolor and multisample). Importantly, by improving the imaging devices and optimizing the conditions for imaging, such as intensity of excitation and time intervals for image acquisition, the procedure itself was not detrimental to full-term development, although it is a prolonged imaging process. For example, live pups were obtained from embryos to which two different wavelengths of excitation (488 and 561 nm) were applied at 7.5-min intervals for about 70 h, and 51 images were acquired in the z axis at each time point; thus, a total of 56,814 fluorescent images were taken. All the pups were healthy, reproductively normal and not transgenic. Thus, this live-cell imaging technology is safe for full-term mouse development. This offers a novel approach for developmental and reproductive research in that it enables both retrospective and prospective analyses of development. It might also be applicable to assessment of embryo quality in fields such as human reproductive technology and production animal research. Key words: Embryo assessment, Full-term development, Live-cell imaging, Preimplantation embryo, Spinning-disk confocal microscopy (J. Reprod. Dev. 55: [343][344][345][346][347][348][349][350] 2009) ammalian preimplantation development is a process in which the union of two highly specialized gametes endows the resulting zygote with the potential to form undifferentiated cell types. This is followed by cell proliferation and the beginning of differentiation during the brief period leading up to implantation. In these processes, a number of cellular components and structures are regulated spatially and temporally, as seen in repeated cell division, cell cycle progression and genomic reprogramming via global epigenetic modifications [1]. Hence, many researchers have been attracted to these fundamental biological processes. However, the numbers of oocytes and embryos that can be collected are very limited, especially in mammals. Therefore, analysis of molecular mechanisms is hampered because of difficulties in conducting biochemical analyses on sufficient materials. Also, immunostaining methods ...

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“…Beside advanced maternal age (AMA), the main indications for PCS or PGS are repeated implantation failure (RIF), repeated miscarriage (RM; usually at least three previous miscarriages) and severe male factor infertility (SMF; usually defined as abnormal semen parameters) [9,10].…”

Section: Introductionmentioning

confidence: 99%

Are zona pellucida laser drilling and polar body biopsy safe for in vitro matured oocytes?

Hammoud

Molina-Gomès

Albert

et al. 2010

J Assist Reprod Genet

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Introduction Preconception diagnosis requires first polar body biopsy. When the hole in the zona pellucida is made with a laser beam, heat propagation could, like the biopsy itself, be deleterious. Our aim was to evaluate the effect of this technique on human in vitro matured oocyte and embryo development. Methods One hunded fifty five retrieved immature oocytes from 75 women, matured in vitro, were distributed in 3 groups: 50 oocytes in a control group, without laser drilling and first polar body biopsy, 52 oocytes in a group with only laser drilling, and 53 oocytes in a group with both laser drilling and first polar body biopsy. Safety was evaluated using four criteria: [1] oocyte lysis rate, [2] oocyte activation rate, [3] oocyte development after calcium ionophore treatment, [4] and embryo chromosome breakage incidence after Tarkowski preparation. Results No difference in the four criteria was observed between the 3 oocyte groups.Conclusions We did not find evidence of deleterious effect of laser drilling and first polar body biopsy on in vitro matured oocytes, according to our criteria.

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Embryo aneuploidy screening for repeated implantation failure and unexplained recurrent miscarriage

Cited by 36 publications

References 115 publications

Gamete cytogenetic study in couples with implantation failure: aneuploidy rate is increased in both couple members

Gamete cytogenetic study in couples with implantation failure: aneuploidy rate is increased in both couple members

Long-Term, Six-Dimensional Live-Cell Imaging for the Mouse Preimplantation Embryo That Does Not Affect Full-Term Development

Are zona pellucida laser drilling and polar body biopsy safe for in vitro matured oocytes?

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