EM investigation of myoblast origin in regenerating hamster skeletal muscle explants

Naidoo, Poovalingam R.

doi:10.1016/1047-8477(92)90047-e

Cited by 2 publications

(11 citation statements)

References 28 publications

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“…Ic) with deep invaginations, which seemed to imbibe cytoplasmic elements in the area of invaginations (Fig. ld) were a surprisingly common feature of regenerating muscle, as reported in earlier work (Naidoo, 1992). This imbibition would explain the electron dense material adjacent to invaginations, and would perhaps also explain the origin of the many electron dense or heterochromatic myonuclei.…”

Section: Resultssupporting

confidence: 77%

“…No.9. 1993 hamster muscle (Naidoo, 1992), except that the human muscle seemed more susceptible to FIGURE 1. Electron micrographs (a-d) represent some myonuclear morphology in explants during culture .…”

Section: Resultsmentioning

confidence: 99%

“…Human muscle was subjected to the same techniques described previously (Naidoo, 1992), except that incubation was prolonged for 10 days as human muscle in vitro studies take longer to show regenerative changes. In brief, the culture technique involved the mincing of cleaned muscle and growing them in flasks containing DMEM base medium with chicken embryo extract, foetal bovine serum and antibiotic / antimycotic mixture.…”

Section: Methodsmentioning

confidence: 99%

“…Myoblast transplant (Partridge et aI., 1989;Karpati, 1990;Law et al, 1990;Gussoni et al, 1992;Huard et al, 1992 ) and gene transfer (Acsadi et al, 1991) methods are currently being investigated as possible future therapies. Because these therapies depend on muscle regeneration, this study attempted to clarify the position of the precursor cells or presumptive myoblasts (PM) during regeneration in human skeletal muscle and to ascertain if the findings are similar to that found in the earlier work on hamster muscle (Naidoo, 1992). The need for this work was clearly shown by Sloper and Partridge (1980) who concluded: "The source of the mononuclear muscle cell precursors is still uncertain.…”

Section: Introductionmentioning

confidence: 95%

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Em evidence of myoblast origin in regenerating human skeletal muscle explants

Naidoo

1993

Cell Biology International

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EM study of cultured human skeletal muscle explants on 10 consecutive days after incubation made possible a record for the first time, the early events occurring during regeneration. After incubation, normal myonuclei underwent activation and dense granulation. Some myonuclei showed early transformation to presumptive myoblasts. The conclusion was that myonuclei transformed into myoblasts which developed into satellite cells (SC). These SC of myonuclear origin, proliferated, and fused forming myotubes that matured into myofibres, replacing damaged muscle. The findings have new implications for the current myoblast/cell transplant and gene transfer therapy research which may provide possible answers for muscular dystrophy in the future.

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Section: Resultssupporting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

See 2 more Smart Citations

Em evidence of myoblast origin in regenerating human skeletal muscle explants

Naidoo

1993

Cell Biology International

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“…Muscle transplantation, as an in vivo experimental method, is frequently used for studying muscular dystrophy (Laird & Timmer, 1965;Rolston, 1972), muscle-nerve interaction (Carlson & Gutmann, 1975), and muscle cell regeneration (Hoh & Hughes, 1988), but for studying myogenesis this method has not yet attracted so much attention as the in vitro culture (Jasmin et al, 1984;Naidoo, 1992).…”

confidence: 99%

Persistent expression of tissue-specific troponin T isoforms in transplanted chicken skeletal muscle

Yao

Kirinoki

Hirabayashi

1994

J Muscle Res Cell Motil

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This study attempted to investigate the expression of skeletal muscle troponin T isoforms in chicken reared for six months after muscle transplantations of breast muscle into leg muscle, leg muscle into breast muscle, and slow muscle into breast and leg muscles of the same animal. The regenerated muscle after transplantation was studied by histological observation, two-dimensional SDS-polyacrylamide gel electrophoresis, and immunoblotting with anti-troponin T antibodies. Persistent expression of troponin T isoforms specific to donor tissue was observed in the regenerated muscle, and compared with their expression in the normal developing muscles. During the regeneration, the cells grew up and expressed troponin T isoforms in a manner similar to that in normal developing muscles, and on around the 178th day after the transplantation, the regenerated muscle expressed the adult type troponin T isoforms. Based on the troponin T isoforms expressed in the transplants, we consider that one type of skeletal muscle has some inherent potential to grow in and coexist with other types for a long term.

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EM investigation of myoblast origin in regenerating hamster skeletal muscle explants

Cited by 2 publications

References 28 publications

Em evidence of myoblast origin in regenerating human skeletal muscle explants

Em evidence of myoblast origin in regenerating human skeletal muscle explants

Persistent expression of tissue-specific troponin T isoforms in transplanted chicken skeletal muscle

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