Elution Profiles and Molecular Weights of Placental Cystine Aminopeptidase in Animals.

Ikenaga, Hidenori; Ono, Kenichiro; Tomoda, Isamu

doi:10.1292/jvms.56.1139

Cited by 1 publication

(3 citation statements)

References 14 publications

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“…There was a difference in placental LNPEP with the body and pregnant horn having higher levels than the non-pregnant horn. Our findings are consistent with a study that reported the molecular weight of LNPEP as 115 kDa in an equine placental extract 31 , and a recent transcriptomic study has demonstrated the expression of LNPEP in the equine placenta throughout gestation 42 . In our other tissues the highest concentration of LNPEP per gram of protein was present in the myometrium and placenta, which is similar to other species 16 , 18 , 19 .…”

Section: Discussionsupporting

confidence: 93%

“…www.nature.com/scientificreports/ the molecular weight of LNPEP as 115 kDa in an equine placental extract 31 , and a recent transcriptomic study has demonstrated the expression of LNPEP in the equine placenta throughout gestation 42 . In our other tissues…”

Section: Data Availabilitymentioning

confidence: 99%

“…Those authors suggested that the LNPEP was most likely derived from serum transudation 30 , but no studies were performed to identify if the equine endometrium expressed LNPEP. Oxytocinase, with a molecular weight of 115 kDa, was identified in full term equine placental extracts 31 . Additionally, a study in 40 pregnant and 10 non-pregnant mares that measured monthly changes in the circulating levels of LNPEP in plasma via a colorimetric test did not find any differences between pregnant and non-pregnant mares 32 .…”

Section: Introductionmentioning

confidence: 99%

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Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare

Amorim

Dong

Byron

et al. 2023

Sci Rep

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Oxytocin is a hormone with functions in: reproduction, maternal bonding, milk ejection, and feeding/social behavior, and is reported to be present in a variety of tissues. Our goal is to characterize oxytocin and leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase), a key regulator of oxytocin in mares. We measured serum and tissue LNPEP by ELISA from ovulation (D0) until D21–22 in non-pregnant (n = 5) and pregnant mares (n = 6); and in periparturient and postpartum mares (n = 18). Placenta (n = 7) and homogenized tissue of diestrus mares (n = 6) were evaluated using protein determinations and LNPEP ELISAs. Identification of LNPEP and OXT protein in tissues was also performed via western blot, immunohistochemistry and liquid chromatography-mass spectrometry (LC-MS/MS). Furthermore, in situ hybridization was performed for LNPEP and OXT on endometrium, myometrium, pituitary and corpus luteum (CL). Serum LNPEP concentration were similar. Placental LNPEP U/mg protein was highest in the body and pregnant horn. The highest to lowest LNPEP U/mg protein by tissue were: myometrium > follicle wall > endometrium > kidney > CL > liver. Oxytocin was identified in the equine pituitary, CL and placenta and is likely to act in autocrine or paracrine manner, while LNPEP may act systemically and locally to regulate the availability of OXT.

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