Elution‐extrusion Counter‐current Chromatography Separation of Two New Benzyl Ester Glucosides and Three Other High‐polarity Compounds from the Tubers of <i>Pleione bulbocodioides</i>

Wang, Yang; Guan, Shu‐Hong; Ren, Feng; Zhang, Jingxian; Li, Qing; Chen, Xiaohui; Bi, Kaishun; Guo, De‐An

doi:10.1002/pca.2453

Cited by 18 publications

(12 citation statements)

References 21 publications

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“…This was assumed to be the effects of the wild environment and growth years. On account of the difficulty to obtain high-polarity compounds 117 , 118 , 123 and 145 , Wang [45] developed a rapid and efficient method Elution-extrusion Counter-current Chromatography Separation (EECCC): the solvent system composed of n -butanol, ethanol and water with a volume ratio of 20:1:20. The upper phase was stationary phase, the lower phase was mobile phase at a flow rate of 1.5 mL·min −1 , with a rotation speed of 850 rpm at temperature of 35 °C.…”

Section: Chemical Constituentsmentioning

confidence: 99%

Chemical Constituents and Biological Activity Profiles on Pleione (Orchidaceae)

Chen

et al. 2019

Molecules

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Pleione (Orchidaceae) is not only famous for the ornamental value in Europe because of its special color, but also endemic in Southern Asia for its use in traditional medicine. A great deal of research about its secondary metabolites and biological activities has been done on only three of 30 species of Pleione. Up to now, 183 chemical compounds, such as phenanthrenes, bibenzyls, glucosyloxybenzyl succinate derivatives, flavonoids, lignans, terpenoids, etc., have been obtained from Pleione. These compounds have been demonstrated to play a significant role in anti-tumor, anti-neurodegenerative and anti-inflammatory biological activities and improve immunity. In order to further develop the drugs and utilize the plants, the chemical structural analysis and biological activities of Pleione are summarized in this review.

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Section: Chemical Constituentsmentioning

confidence: 99%

Chemical Constituents and Biological Activity Profiles on Pleione (Orchidaceae)

Chen

et al. 2019

Molecules

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“…Compared to other chromatographic methods, centrifugal partition chromatography (CPC) is compatible with green chemistry criteria since it does not use any polluting solid support such as silica. Moreover, it allows the complete recovery of the injected extract without degradation and only requires a limited amount of organic solvents [ 19 ], and it turned to be a versatile method of separation for the isolation of glycosides [ 20 – 21 ].…”

Section: Introductionmentioning

confidence: 99%

Three new trixane glycosides obtained from the leaves of Jungia sellowii Less. using centrifugal partition chromatography

Azevedo

Faqueti

Kritsanida

et al. 2016

Beilstein J. Org. Chem.

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Summary Jungia sellowii (Asteraceae) is a shrub that grows in Southern Brazil and polar extract of its leaves presents anti-inflammatory properties. Cyperane, guaiane, nortrixane, and trixane sesquiterpene types were reported as the main metabolites in Jungia species. This work aims to describe the isolation and identification of sesquiterpenes in the leaves of J. sellowii using liquid–liquid partition and centrifugal partition chromatography. Thus, the crude extract of fresh leaves of J. sellowii was partitioned with hexane, dichloromethane, ethyl acetate and butanol, respectively. The butanol fraction was then subjected to a selected ternary system optimized for the CPC (centrifugal partition chromatography): ethyl acetate–ethanol–water (9:2:10, v/v/v). The separation was carried out isocratically at a flow rate of 25 mL/min at 1200 rpm, affording seven fractions A to G. TLC of fractions B, C and F displayed a single spot corresponding to three new glycosylated sesquiterpenoids. Their structures were established by using spectroscopic data in comparison to those reported in the literature. Furthermore, the isolates were evaluated for their leishmanicidal and cytotoxic effects. No cytotoxic effect was observed against the three cancer cell lines (HL60, JURKAT and REH), but compound 1 showed a weak antiprotozoal activity. Liquid–liquid partition and CPC turned to be a versatile technique of glycoside purification which is environmentally friendly and requires a limited amount of organic solvents.

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“…This latter appeared to be the most efficient and fast way to purify AH. Indeed, counter‐current chromatography (CCC) techniques, such as CPC, are efficient for the separation of polar extracts (Wang et al, ) and were already successfully applied to the isolation of iridoids (Kim and Kim, ; Marković et al, ; Tong et al, ). Moreover, scale‐up procedure with CPC is considered as quasi‐linear and therefore is suitable for large amount of extracts as pilot and industrial scale instruments are available.…”

Section: Resultsmentioning

confidence: 99%

HPLC‐ELSD Quantification and Centrifugal Partition Chromatography Isolation of 8‐O‐Acetylharpagide from Oxera coronata (Lamiaceae)

Remeur

Borgne

Gauthier

et al. 2016

Phytochemical Analysis

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Elution‐extrusion Counter‐current Chromatography Separation of Two New Benzyl Ester Glucosides and Three Other High‐polarity Compounds from the Tubers of Pleione bulbocodioides

Abstract: Elution-extrusion counter-current chromatography could be used as a useful tool for the separation of high-polarity compounds such as gastrodin and benzyl ester glucosides and the enrichment of the minor ones.

Cited by 18 publications

References 21 publications

Chemical Constituents and Biological Activity Profiles on Pleione (Orchidaceae)

Chemical Constituents and Biological Activity Profiles on Pleione (Orchidaceae)

Three new trixane glycosides obtained from the leaves of Jungia sellowii Less. using centrifugal partition chromatography

HPLC‐ELSD Quantification and Centrifugal Partition Chromatography Isolation of 8‐O‐Acetylharpagide from Oxera coronata (Lamiaceae)

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