Elution Control of Microparticles with a Coupled Acoustic-Gravity Field and Orthogonal Laminar Flow

Masudo, Takashi; Okada, Tetsuo

doi:10.2116/analsci.20.753

Cited by 14 publications

(12 citation statements)

References 12 publications

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“…28,29 The levitation coordinate of a particle in this field is a function of the density and compressibility of the particle. If these acoustic properties of a particle are altered as a result of a reaction, the levitation coordinate changes accordingly.…”

Section: Introductionmentioning

confidence: 99%

Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles

et al. 2017

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Herein, we propose a concept for sensing based on density changes of microparticles (MPs) caused by a biochemical reaction. The MPs are levitated by a combined acoustic-gravitational force at a position determined by the density and compressibility. Importantly, the levitation is independent of the MPs sizes. When gold nanoparticles (AuNPs) are bound on the surface of polymer MPs through a reaction, the density of the MPs dramatically increases, and their levitation position in the acoustic-gravitational field is lowered. Because the shift of the levitation position is proportional to the number of AuNPs bound on one MP, we can determine the number of molecules involved in the reaction. The avidinbiotin reaction is used to demonstrate the effectiveness of this concept. The number of molecules involved in the reaction is very small because the reaction space is small for an MP; thus, the method has potential for highly sensitive detection.

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Section: Introductionmentioning

confidence: 99%

Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles

et al. 2017

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“…In the usual flow separation methods, appropriate chemical (chromatography) or physical (FFF) separation fields are created, which retain solutes to different extents and separate them. A variety of stationary phases for chromatography 2,[12][13][14] and physical forces for FFF [15][16][17][18][19] have been exploited, and have provided different capabilities and selectivity from those performed by conventional separation. In contrast, a separation method that requires neither chemical interactions nor special external fields should also be useful because of its instrumental simplicity.…”

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confidence: 99%

Solute Distribution Coupled with Laminar Flow in Wide-Bore Capillaries: What Can Be Separated without Chemical or Physical Fields?

et al. 2005

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A number of researches have been devoted to the developments of novel separation principles and methodology, because material separation is one of the most important fundamentals in scientific and technological disciplines. In particular, flow separation methods, such as chromatography, 1,2 electrophoresis, [3][4][5][6][7] and field flow fractionation (FFF), [8][9][10] have been extensively used in various fundamental and practical fields, and have facilitated the advancements of modern science. These methods cover separation of almost all classes of materials. A number of dissolved molecules, from simple ions to macromolecules, can be separated by chromatography or electrophoresis, FFF and electrophoresis being capable of resolving even particles. The dimensions of solutes to be separated by these methods thus range from 10 -10 m to 10 -5 m. This situation may suggest that separation science and technology have been completely matured. However, studying new separation principles is still important, because they can allow the separation based on material properties that have not been utilized for conventional methods, provide higher performance and more efficient separation than existing means, and facilitate the understanding of materials through separation processes.According to chromatographic theories, the intrinsic separation performance of liquid chromatography is restricted in comparison with that of gas chromatography because of much lower solute diffusion in liquid phases. 11However, a liquid flow obviously provides versatile ways for material separation because liquids have higher density and dissolution power than gases, and thus has made possible application to various types of solutes with wide ranges in molecular weights and sizes. In the usual flow separation methods, appropriate chemical (chromatography) or physical (FFF) separation fields are created, which retain solutes to different extents and separate them. A variety of stationary phases for chromatography 2,[12][13][14] and physical forces for FFF [15][16][17][18][19] have been exploited, and have provided different capabilities and selectivity from those performed by conventional separation. In contrast, a separation method that requires neither chemical interactions nor special external fields should also be useful because of its instrumental simplicity. Hydrodynamic chromatography (HDC) is such a case, which allows separation of particles or macromolecules according to their hydrodynamic sizes just by passing them through a narrow channel. [20][21][22][23][24] Particles with larger diameter for example cannot approach the channel wall, and thus flow through the channel faster than the smaller particles, when the laminar flow profile is maintained therein. Although the applicability of this method was very restricted, recent developments of chip technology have brought about a remarkable progress. 23,24 If a capillary of radius a is used for HDC, the time for the elution of a particle of radius rp is given bywhere t0 is the time required ...

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“…We studied a coupled acoustic-gravitational (CAG) field for particle separation. − One of the key features of this field is that it resolves the density and compressibility of particles but does not recognize their sizes . Therefore, a reaction that affects the acoustic properties of an MP can be detected by the CAG field; we do not need to consider size changes during the reaction process.…”

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Zeptomole Detection Scheme Based on Levitation Coordinate Measurements of a Single Microparticle in a Coupled Acoustic-Gravitational Field

2018

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We present a novel analytical principle in which an analyte (according to its concentration) induces a change in the density of a microparticle, which is measured as a vertical coordinate in a coupled acoustic-gravitational (CAG) field. The density change is caused by the binding of gold nanoparticles (AuNP's) on a polystyrene (PS) microparticle through avidin-biotin association. The density of a 10-μm PS particle increases by 2% when 500 100-nm AuNP's are bound to the PS. The CAG can detect this density change as a 5-10 μm shift of the levitation coordinate of the PS. This approach, which allows us to detect 700 AuNP's bound to a PS particle, is utilized to detect biotin in solution. Biotin is detectable at a picomolar level. The reaction kinetics plays a significant role in the entire process. The kinetic aspects are also quantitatively discussed based on the levitation behavior of the PS particles in the CAG field.

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Elution Control of Microparticles with a Coupled Acoustic-Gravity Field and Orthogonal Laminar Flow

Cited by 14 publications

References 12 publications

Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles

Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles

Solute Distribution Coupled with Laminar Flow in Wide-Bore Capillaries: What Can Be Separated without Chemical or Physical Fields?

Zeptomole Detection Scheme Based on Levitation Coordinate Measurements of a Single Microparticle in a Coupled Acoustic-Gravitational Field

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