Elution Behavior of Carbohydrates Using Core-Shell Ion-Exchange Resin St-70 with Different Degrees of Cross-Linking in the Porous Shell

Abstract

A novel core-shell ion-exchange resin (St-70) having a core-shell monomer weight ratio of 30:70 was prepared for use in highperformance liquid chromatography (HPLC), and the effect of the degree of cross-linking (10-55%) of the porous shell on the separation of carbohydrates was examined. A mixture of inositol, glucose, fructose, and sucrose was separated under alkaline conditions (100 and 150 mmol/L NaOH) at flow rates of 0.3-0.7 mL/min using St-70, which exhibited a degree of cross-linking of 10, 40, and 55%. The retention time of carbohydrates decreased with increasing of the degree of cross-linking in the porous shell. The theoretical plate number (N) of glucose, fructose, and sucrose decreased with decreasing the degree of cross-linking when 100 mmol/L NaOH eluent was used at flow rates of 0.3 and 0.5 mL/min.

Fibrous Polyimide Stationary Phase for the Separation of Aromatic Compounds in Reversed-Phase Liquid Chromatography

“…Synthetic polymer materials have been recently introduced to chromatographic method [30,31]. In conventional silicagel-based stationary phases such as a typical ODS phase, functional groups (such as the ligands for the separation) bond with silanol groups on the surface of silicagel particles.…”