Eluted zinc ions stimulate osteoblast differentiation and mineralization in human dental pulp stem cells for bone tissue engineering

Yusa, Kosuke; Yamamoto, Osamu; Iino, Mitsuyoshi; Takano, Hiroshi; Fukuda, Masayuki; Qiao, Zhiwei; Sugiyama, Toshihiro

doi:10.1016/j.archoralbio.2016.07.010

Cited by 56 publications

(33 citation statements)

References 40 publications

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“…29 It was previously reported that the treatment of mesenchymal SCs with ZnO NPs could induce osteogenic factors such as osteocalcin and ALP activity, and the production of extracellular matrix, mainly collagen. 30,31 By providing an expanded surface area and incorporation of various membrane proteins in cultured cells via the application of ZnO NPs, it seems that the osteoblast differentiation would be accelerated by modulating ALP activity. Similar to the effect of ZnO particles, the films coated by zirconia exhibited a profound effect on osteoblast-like MG63 cells, ALP activity and the transcription level of osteogenic markers.…”

Section: Discussionmentioning

confidence: 99%

In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components

Rahimi

Salarinasab

Ghasemi

et al. 2019

J Dent Res Dent Clin Dent Prospects

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Background. The aim of this in vitro study was to investigate the effect of zinc oxide (ZnO) and zirconium oxide (ZrO2) microparticles (MPs) and nanoparticles (NPs) in combination with white Portland cement (WPC) on odontogenic capacity of human dental pulp stem cells over a period of 21 days. Methods. Synthesized ZnO and ZrO2 particles were characterized using scanning electron microscopy and transmission electron microscopy. The viability of human dental pulp stem cells was measured by a 3-(4,5-dimethylthiazolyl-2-yl)-2,5- diphenyltetrazolium bromide assay at 7-, 14- and 21-day intervals after seeding on WPC disks enriched with ZnO and ZrO2 MPs and NPs. Odontogenic potential of ZnO and ZrO2 particles in combination with WPC was investigated by alkaline phosphatase (ALP) activity and ionized calcium level of supernatant culture media at different time intervals. Data were analyzed using one-way ANOVA and post hoc Tukey tests. Results. All the materials exhibited cell viability over a 21-day period, except for WPC with ZnO NPs on day 7, although it was not statistically significant (P>0.05). The ALP activity and ionized calcium level increased in all the groups compared to the control group (P<0.05). ZnO NPs had superior effect on odontogenic activity and calcium ion release compared to ZnO MPs (P=0.046). There was no significant difference between ZrO2 MPs and NPs in odontogenic activity (P>0.05). Conclusion. WPC enriched with ZnO and ZrO2 increased ALP activity and calcium ion release of human dental pulp stem cells over a period of 21 days in vitro.

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Section: Discussionmentioning

confidence: 99%

In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components

Rahimi

Salarinasab

Ghasemi

et al. 2019

J Dent Res Dent Clin Dent Prospects

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“…BM-MSCs derived from maxilla enhance the osteogenesis capacity of PDLSC, in vitro [32]. The BM-MSCs have an elevated in vitro capacity of osteogenesis when treated with zinc ions as is shown by elevated levels of type I collagen, alkaline phosphatase (ALP), osteocalcin (OCN) and Runx2 expression [33].…”

Section: Types Of Stem Cells Used In Regenerative Medicinementioning

confidence: 99%

The Potential of Different Origin Stem Cells in Modulating Oral Bone Regeneration Processes

Buduru

Gulei

Zimța

et al. 2019

Cells

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Tissue engineering has gained much momentum since the implementation of stem cell isolation and manipulation for regenerative purposes. Despite significant technical improvements, researchers still have to decide which strategy (which type of stem cell) is the most suitable for their specific purpose. Therefore, this short review discusses the advantages and disadvantages of the three main categories of stem cells: embryonic stem cells, mesenchymal stem cells and induced pluripotent stem cells in the context of bone regeneration for dentistry-associated conditions. Importantly, when deciding upon the right strategy, the selection needs to be made in concordance with the morbidity and the life-threatening level of the condition in discussion. Therefore, even when a specific type of stem cell holds several advantages over others, their availability, invasiveness of the collection method and ethical standards become deciding parameters.

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“…Now that stem cells can be generated from somatic cells through reprogramming protocols based on signal transduction and key transcription factors [70]; adult somatic cells reprogrammed as induced pluripotent stem cells (iPSCs), as shown in Figure 3, can be another potential source of cells for bone tissue engineering application [71].…”

Section: Scaffold Transformation For Regenerative Applicationmentioning

confidence: 99%

The Regenerative Applicability of Bioactive Glass and Beta-Tricalcium Phosphate in Bone Tissue Engineering: A Transformation Perspective

Lowe

Ottensmeyer

et al. 2019

JFB

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The conventional applicability of biomaterials in the field of bone tissue engineering takes into consideration several key parameters to achieve desired results for prospective translational use. Hence, several engineering strategies have been developed to model in the regenerative parameters of different forms of biomaterials, including bioactive glass and β-tricalcium phosphate. This review examines the different ways these two materials are transformed and assembled with other regenerative factors to improve their application for bone tissue engineering. We discuss the role of the engineering strategy used and the regenerative responses and mechanisms associated with them.

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Eluted zinc ions stimulate osteoblast differentiation and mineralization in human dental pulp stem cells for bone tissue engineering

Cited by 56 publications

References 40 publications

In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components

In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components

The Potential of Different Origin Stem Cells in Modulating Oral Bone Regeneration Processes

The Regenerative Applicability of Bioactive Glass and Beta-Tricalcium Phosphate in Bone Tissue Engineering: A Transformation Perspective

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