Elucidation of the role of LH and FSH during neonatal testicular development and growth in the boar

Wells, R; Kenny, Alexander D.; Duckett, Richard; Wreford, Nigel G.; Johnston, Susan J; D’Occhio, M. J.

doi:10.1016/j.anireprosci.2012.11.005

Cited by 9 publications

(3 citation statements)

References 27 publications

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“…In other studies, serum T levels were significantly reduced by Cu treatment; however, it has also been found that Cu exposure does not affect serum T levels (Zhang et al 2016 ; Chen et al 2020 ). The LH and FSH can respectively induce proliferation of testicular Leydig cell and Sertoli cell, and LH additionally supports a normal increase in the testicular size (Wells et al 2013 ). The TH is important for growth, development, and metabolism, and testicular development is also highly dependent on TH status (Hernandez 2018 ).…”

Section: Discussionmentioning

confidence: 90%

Long-term copper exposure promotes apoptosis and autophagy by inducing oxidative stress in pig testis

Chen

Liao

et al. 2021

Environ Sci Pollut Res

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Copper (Cu) is a heavy metal which is being used widely in the industry and agriculture. However, the overuse of Cu makes it a common environmental pollutant. In order to investigate the testicular toxicity of Cu, the pigs were divided into three groups and were given Cu at 10 (control), 125, and 250 mg/kg body weight, respectively. The feeding period was 80 days. Serum hormone results showed that Cu exposure decreased the concentrations of follicular stimulating hormone (FSH) and luteinizing hormone (LH) and increased the concentration of thyroxine (T4). Meanwhile, Cu exposure upregulated the expression of Cu transporter mRNA (Slc31a1, ATP7A, and ATP7B) in the testis, leading to increase in testicular Cu and led to spermatogenesis disorder. The Cu exposure led to an increased expression of antioxidant-related mRNA (Gpx4, TRX, HO-1, SOD1, SOD2, SOD3, CAT), along with increase in the MDA concentration in the testis. In LG group, the ROS in the testis was significantly increased. Furthermore, the apoptotic-related mRNA (Caspase3, Caspase8, Caspase9, Bax, Cytc, Bak1, APAF1, p53) and protein (Active Caspase3) and the autophagy-related mRNA (Beclin1, ATG5, LC3, and LC3B) expression increased after Cu exposure. The mitochondrial membrane potential in the testicular tissue decreased, while the number of apoptotic cells increased, as a result of oxidative stress. Overall, our study indicated that the Cu exposure promotes testicular apoptosis and autophagy by mediating oxidative stress, which is considered as the key mechanism causing testicular degeneration as well as dysfunction.

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Section: Discussionmentioning

confidence: 90%

Long-term copper exposure promotes apoptosis and autophagy by inducing oxidative stress in pig testis

Chen

Liao

et al. 2021

Environ Sci Pollut Res

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“…Debra M. Yeh et al used mice to demonstrate that both GNRH1 and pituitary adenylate cyclase-activating polypeptide (PACAP) can promote the secretion of FSH and LH through this pathway [ 49 ]. Wells R et al found that treatment with GnRH agonists promoted the secretion of FSH and LH in the pituitaries of pigs and affected the growth of testes in boars [ 50 ]. In our study, changes in FSH and LH in rats after GnRH treatment were detected by ELISA.…”

Section: Discussionmentioning

confidence: 99%

Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH

Guo

Yuan

et al. 2021

Animals

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The pituitary gland, an important endocrine organ, can secrete a variety of reproductive hormones under the action of hypothalamus-secreted gonadotropin-releasing hormone (GnRH) and plays important roles in animal reproduction. Circular RNAs (circRNAs) are a class of RNA molecules with stable covalently closed circular structures. CircRNAs are equipped with miRNA response elements (MREs), which can regulate the expression of target genes by competitively binding miRNAs. However, whether the expression levels of circRNAs in the pituitary gland change under the action of GnRH and whether such changes can further affect the secretion of reproductive hormones are still unclear. In this study, we performed RNA sequencing (RNA-Seq) of GnRH-treated rats to identify differentially expressed circRNAs. The results revealed 1433 related circRNAs, 14 of which were differentially expressed. In addition, we randomly selected five differentially expressed circRNAs and tested their relative expression levels by RT-qPCR, the results of which were consistent with the RNA sequencing results. Finally, we predicted targeted relationships between the differentially expressed circRNAs and FSHb-LHb-associated miRNAs. In all, a total of 14 circRNAs were identified that may act on the secretion and regulation of reproductive hormones in GnRH-treated rats. Our expression profiles of circRNAs in the anterior pituitaries of rats treated with GnRH can provide insights into the roles of circRNAs in mammalian development and reproduction.

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“…This compound provides negative feedback to the anterior pituitary to decrease FSH secretion. LH stimulates Leydig cells to produce T2, which provides negative feedback to the anterior pituitary and hypothalamus [ 47 ]. In this study, a slight fluctuation in LH level was observed in the serum after H 2 O 2 injection.…”

Section: Discussionmentioning

confidence: 99%

Effects of Glutamate and Aspartate on Serum Antioxidative Enzyme, Sex Hormones, and Genital Inflammation in Boars Challenged with Hydrogen Peroxide

Deng

et al. 2016

Mediators of Inflammation

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Background. Oxidative stress is associated with infertility. This study was conducted to determine the effects of glutamate and aspartate on serum antioxidative enzymes, sex hormones, and genital inflammation in boars suffering from oxidative stress. Methods. Boars were randomly divided into 4 groups: the nonchallenged control (CON) and H2O2-challenged control (BD) groups were fed a basal diet supplemented with 2% alanine; the other two groups were fed the basal diet supplemented with 2% glutamate (GLU) or 2% aspartate (ASP). The BD, GLU, and ASP groups were injected with hydrogen peroxide (H2O2) on day 15. The CON group was injected with 0.9% sodium chloride solution on the same day. Results. Dietary aspartate decreased the malondialdehyde (MDA) level in serum (P < 0.05) compared with the BD group. Additionally, aspartate maintained serum luteinizing hormone (LH) at a relatively stable level. Moreover, glutamate and aspartate increased transforming growth factor-β1 (TGF-β1) and interleukin-10 (IL-10) levels in the epididymis and testis (P < 0.05) compared with the BD group. Conclusion. Both glutamate and aspartate promoted genital mRNA expressions of anti-inflammatory factors after oxidative stress. Aspartate more effectively decreased serum MDA and prevented fluctuations in serum sex hormones after H2O2 challenge than did glutamate.

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Elucidation of the role of LH and FSH during neonatal testicular development and growth in the boar

Cited by 9 publications

References 27 publications

Long-term copper exposure promotes apoptosis and autophagy by inducing oxidative stress in pig testis

Long-term copper exposure promotes apoptosis and autophagy by inducing oxidative stress in pig testis

Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH

Effects of Glutamate and Aspartate on Serum Antioxidative Enzyme, Sex Hormones, and Genital Inflammation in Boars Challenged with Hydrogen Peroxide

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