Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression

Abstract

Lippia citriodora ethanolic extract (VEE) and verbascoside (Vs), a phenypropanoid glycoside, have been demonstrated to exert relaxant and anxiolytic properties. However, the molecular mechanisms behind their effects are still unclear. In this work, we studied the effects and action mechanisms of VEE and Vs in vivo and in vitro, on human neurotypic SH-SY5Y cells.TST was conducted on mice treated orally with VEE (25, 50 and 100 mg/Kg), Vs (2.5 and 5 mg/Kg), Bupropion (20 mg/Kg) and Milli-Q water. Higher dose of VEE-treated mice showed an increase of immobility time compared to control groups, indicating an induction of relaxation. This effect was found to be induced by regulation of genes playing key roles in calcium homeostasis (calcium channels), cyclic AMP (cAMP) production and energy metabolism. On the other hand, low doses of VEE and Vs showed an antidepressant-like effect and was confirmed by serotonin, noradrenalin, dopamine and BDNF expressions. Finally, VEE and Vsenhancedcell viability, mitochondrial activity and calcium uptake in vitro confirming in vivo findings. Our results showed induction of relaxation and antidepressant-like effects depending on the administered dose of VEE and Vs, through modulation of cAMP and calcium.

Microarray analysis of verbenalin-treated human amniotic epithelial cells reveals therapeutic potential for Alzheimer’s Disease

“…Verbenalin, one of the major constituents of this plant, has been reported to exhibit sleep-promoting and antioxidant activities [20,22,23]. In our previous study, we have reported relaxation and anti-depressant effects of lemon verbena (Lippia citriodora) extract, rich in verbascoside and verbenalin, both in the in vitro and in vivo models [24]. In the present study, we have treated hAECs with verbenalin for seven days and conducted microarray analysis to investigate the changes in gene expression and to explore its therapeutic potential for AD.…”

Luteolin Modulates Neural Stem Cells Fate Determination: In vitro Study on Human Neural Stem Cells, and in vivo Study on LPS-Induced Depression Mice Model

“…Sabti et al reported that antidepressants targeting the expression of serotonin and noradrenaline only present limitations and that accordingly antidepressant drugs targeting the dopaminergic system have been developed, whereas the antidepressants acting on serotonergic mechanisms lead to enhancement of BDNF levels in rodents ( Sabti et al, 2019 ). Hence, we assessed the mature BDNF levels, noradrenaline and dopamine levels in mice hypothalamus and serotonin level in mice cortex.…”

Integrated analysis of the chemical-material basis and molecular mechanisms for the classic herbal formula of Lily Bulb and Rehmannia Decoction in alleviating depression

“…Verbascoside, a phenypropanoid glycoside, has been demonstrated to exert antidepressant and anxiolytic effects through modulation of cAMP, calcium homeostasis (calcium channels), and energy metabolism [ 35 – 37 ]. Our behavior test results indicted verbascoside at 60 mg/kg displayed anxiolytic and antidepressant activity (Additional file 1 : Figure S4).…”