Elucidation of structure–function relationships in photosynthetic light-harvesting antenna complexes by non-linear polarization spectroscopy in the frequency domain (NLPF)

Lokstein, Heiko; Krikunova, Maria; Teuchner, Klaus; Voigt, B.

doi:10.1016/j.jplph.2010.12.012

Cited by 4 publications

(3 citation statements)

References 57 publications

(89 reference statements)

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“…Our results are in good agreement with the data of non-linear polarization spectroscopy in the frequency domain, which showed that chromophores α84 and β84 are not excitonically coupled in C-PC monomers. 57 Thus, we believe that spontaneous or induced disruption of protein–protein interactions between C-PC αβ 1 monomers leads to an increase in conformational mobility and chromophore isomerization. It should be noted that high urea concentrations affect the viscosity of the solution, so the assessment of the hydrodynamic mobility of C-PC by anisotropy relaxation kinetics [ Fig.…”

Section: Resultsmentioning

confidence: 97%

Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores

Tsoraev

Protasova

Климанова

et al. 2022

Structural Dynamics

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The structural organization of natural pigment-protein complexes provides a specific environment for the chromophore groups. Yet, proteins are inherently dynamic and conformationally mobile. In this work, we demonstrate the heterogeneity of chromophores of C-phycocyanin (C-PC) from Arthrospira platensis. Part of the population of trimeric C-PC is subject to spontaneous disturbances of protein–protein interactions resulting in increased conformational mobility of the chromophores. Upon fluorescence excitation in the visible range, the spectral signatures of these poorly populated states are masked by bulk chromophore states, but the former could be clearly discriminated when the fluorescence is excited by near-infrared quanta. Such selective excitation of conformationally mobile C-PC chromophores is due to the structure of their S1 level, which is characterized by a significantly broadened spectral line. We demonstrate that the anti-Stokes C-PC fluorescence is the result of single-photon absorption. By combining spectral and structural methods, we characterize four distinct states of C-PC chromophores emitting at 620, 650, 665, and 720 nm and assigned the fast component in the anti-Stokes fluorescence decay kinetics in the range of 690–750 nm to the chromophores with increased conformational mobility. Our data suggest that the spectral and temporal characteristics of the anti-Stokes fluorescence can be used to study protein dynamics and develop methods to visualize local environment parameters such as temperature.

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Section: Resultsmentioning

confidence: 97%

Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores

Tsoraev

Protasova

Климанова

et al. 2022

Structural Dynamics

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“…A suitable method to resolve the spectral substructures of complex pigment-protein assemblies, like the photosynthetic light-harvesting antennae, is non-linear polarization spectroscopy in the frequency domain. This is the conclusion of a review by Lokstein et al (2011) who recommend the application of this technique to elucidate structure-function relationships in pigment-protein complexes. Finally, Renger et al (2011b) have theoretically analysed the determination of the flow of excitation energy in the light-harvesting complex by the molecular structure.…”

Section: Regulation Of Plant Primary Metabolismmentioning

confidence: 97%

Regulation of plant primary metabolism

Börner

2011

Journal of Plant Physiology

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“…A novel-much improved-set-up for NLPF spectroscopy was proposed and introduced by Voigt et al (1999). Previous study using NLPF to study pigment-pigment interactions in various systems, including LHCs, was recently reviewed by Lokstein et al (2011).…”

Section: Introductionmentioning

confidence: 99%

Elucidation of structure–function relationships in plant major light-harvesting complex (LHC II) by nonlinear spectroscopy

et al. 2011

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Conventional linear and time-resolved spectroscopic techniques are often not appropriate to elucidate specific pigment-pigment interactions in light-harvesting pigment-protein complexes (LHCs). Nonlinear (laser-) spectroscopic techniques, including nonlinear polarization spectroscopy in the frequency domain (NLPF) as well as step-wise (resonant) and simultaneous (non-resonant) two-photon excitation spectroscopies may be advantageous in this regard. Nonlinear spectroscopies have been used to elucidate substructure(s) of very complex spectra, including analyses of strong excitonic couplings between chlorophylls and of interactions between (bacterio)chlorophylls and "optically dark" states of carotenoids in LHCs, including the major antenna complex of higher plants, LHC II. This article shortly reviews our previous study and outlines perspectives regarding the application of selected nonlinear laser-spectroscopic techniques to disentangle structure-function relationships in LHCs and other pigment-protein complexes.

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Elucidation of structure–function relationships in photosynthetic light-harvesting antenna complexes by non-linear polarization spectroscopy in the frequency domain (NLPF)

Cited by 4 publications

References 57 publications

Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores

Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores

Regulation of plant primary metabolism

Elucidation of structure–function relationships in plant major light-harvesting complex (LHC II) by nonlinear spectroscopy

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