Elucidation of stage specific physiological sensitivity of okra to drought stress through leaf gas exchange, spectral indices, growth and yield parameters

Chaturvedi, Ashish; Surendran, U.; Gopinath, Girish; Chandran, K. Madhava; Nk, Anjali; Ct, Mohamed Fasil

doi:10.1016/j.agwat.2019.05.041

Cited by 35 publications

(30 citation statements)

References 51 publications

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“…Our recent findings also support the results of the present study, where a drought-tolerant onion genotype had higher RWC, MSI, and antioxidant enzyme activity than the sensitive genotype ( Ghodke et al, 2020 ). The findings were further supported by the study of Chaturvedi et al (2019) in okra, where plant water status and cellular membrane stability decreased under drought stress during the crop vegetative and reproductive stage. The phenols are non-enzymatic antioxidants, and there accumulation reported in biotic and abiotic stress ( Hodaei et al, 2018 ).…”

Section: Discussionsupporting

confidence: 55%

Screening of Onion (Allium cepa L.) Genotypes for Drought Tolerance Using Physiological and Yield Based Indices Through Multivariate Analysis

et al. 2021

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Drought is a leading abiotic constraints for onion production globally. Breeding by using unique genetic resources for drought tolerance is a vital mitigation strategy. With a total of 100 onion genotypes were screened for drought tolerance using multivariate analysis. The experiment was conducted in a controlled rainout shelter for 2 years 2017–2018 and 2018–2019 in a randomized block design with three replications and two treatments (control and drought stress). The plant was exposed to drought stress during the bulb development stage (i.e., 50–75 days after transplanting). The genotypes were screened on the basis of the drought tolerance efficiency (DTE), percent bulb yield reduction, and results of multivariate analysis viz. hierarchical cluster analysis by Ward’s method, discriminate analysis and principal component analysis. The analysis of variance indicated significant differences among the tested genotypes and treatments for all the parameters studied, viz. phenotypic, physiological, biochemical, and yield attributes. Bulb yield was strongly positively correlated with membrane stability index (MSI), relative water content (RWC), total chlorophyll content, antioxidant enzyme activity, and leaf area under drought stress. The genotypes were categorized into five groups namely, highly tolerant, tolerant, intermediate, sensitive, and highly sensitive based on genetic distance. Under drought conditions, clusters II and IV contained highly tolerant and highly sensitive genotypes, respectively. Tolerant genotypes, viz. Acc. 1656, Acc. 1658, W-009, and W-085, had higher DTE (>90%), fewer yield losses (<20%), and performed superiorly for different traits under drought stress. Acc. 1627 and Acc. 1639 were found to be highly drought-sensitive genotypes, with more than 70% yield loss. In biplot, the tolerant genotypes (Acc. 1656, Acc. 1658, W-085, W-009, W-397, W-396, W-414, and W-448) were positively associated with bulb yield, DTE, RWC, MSI, leaf area, and antioxidant enzyme activity under drought stress. The study thus identified tolerant genotypes with favorable adaptive traits that may be useful in onion breeding program for drought tolerance.

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Section: Discussionsupporting

confidence: 55%

Screening of Onion (Allium cepa L.) Genotypes for Drought Tolerance Using Physiological and Yield Based Indices Through Multivariate Analysis

et al. 2021

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“… Begum et al (2019) indicated that drought stress decreased plant height, chlorophyll and carotenoid content in Maize (Zea mays). Chaturvedi et al (2019) observed that the significant reduction in relative water content and membrane stability index along with reduced leaf photosynthetic rate explains the possible membrane damage in okra affecting photosynthetic efficiency in drought condition. Several studies have reported that drought stress decreases the protein concentration of plants ( Schwanz et al, 1996 , Heckathorn et al, 1997 ).…”

Section: Introductionmentioning

confidence: 99%

“… Maes et al (2009) reported that Jatropha curcas under different water regimes identified anatomical and morphological changes with an increase in adaxial stomatal ratio in response to drought. Drought negatively affects yield in different crops such as okra ( Mueller et al, 2019 , Chaturvedi et al, 2019 , Ranawake et al, 2012 , Haider et al, 2021 ), faba bean ( Li et al, 2018 ) and chickpea ( Pushpavalli et al, 2015 ). The impact of drought stress was studied on plant growth and yield of okra plant by Abdulrahman and Nadir (2018) .…”

Section: Introductionmentioning

confidence: 99%

Biochar and Arbuscular mycorrhizal fungi mediated enhanced drought tolerance in Okra (Abelmoschus esculentus) plant growth, root morphological traits and physiological properties

Jabborova

Annapurna

Al-Sadi

et al. 2021

Saudi Journal of Biological Sciences

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Drought is a major abiotic factor limiting plant growth and crop production. There is limited information on effect of interaction between biochar and Arbuscular mycorrhizal fungi (AMF) on okra growth, root morphological traits and soil enzyme activities under drought stress. We studied the influence of biochar and AMF on the growth of Okra ( Abelmoschus esculentus ) in pot experiments in a net house under drought condition. The results showed that the biochar treatment significantly increased plant growth (the plant height by 14.2%, root dry weight by 30.0%) and root morphological traits (projected area by 22.3% and root diameter by 22.7%) under drought stress. In drought stress, biochar treatment significantly enhanced the chlorophyll ‘a’ content by 32.7%, the AMF spore number by 22.8% and the microbial biomass as compared to the control. Plant growth parameters such as plant height, shoot and root dry weights significantly increased by AMF alone, by 16.6%, 21.0% and 40.0% respectively under drought condition. Other plant biometrics viz: the total root length, the root volume, the projected area and root diameter improved significantly with the application of AMF alone by 38.3%, 60.0%,16.8% and 15.9% respectively as compared with control. Compared to the control, AMF treatment alone significantly enhanced the total chlorophyll content by 36.6%, the AMF spore number by 39.0% and the microbial biomass by 29.0% under drought condition. However, the highest values of plant growth parameters (plant height, shoot dry weight, root dry weight) and root morphological traits (the total root length, root volume, projected area, root surface area) were observed in the combined treatment of biochar and AMF treatment viz: 31.9%, 34.2%, 60.0% and 68.6%, 66.6%, 45.5%, 41.8%, respectively compared to the control under drought stress. The nitrogen content, total chlorophyll content and microbial biomass increased over un-inoculated control. The soil enzymes; alkaline phosphatase, dehydrogenase and fluorescein diacetate enzyme activities significantly increased in the combined treatment by 55.8%, 68.7% and 69.5%, respectively as compared to the control under drought stress. We conclude that biochar and AMF together is potentially beneficial for cultivation of okra in drought stress conditions.

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“…During 2015–2016, it was estimated that vegetables occupied a major portion of the horticulture sector, approximately an area of 9.6 million ha out of a total production of 166.6 million tones, and an average productivity of 17.4 tons/ha in India, constituting 59% of horticulture production in India [ 21 ]. Okra ( Abelmoschus esculentus L.) is one of the important vegetable crops grown in warm regions of India, because of its high economic and nutritious value.…”

Section: Introductionmentioning

confidence: 99%

Target-Based Physiological Modulations and Chloroplast Proteome Reveals a Drought Resilient Rootstock in Okra (Abelmoschus esculentus) Genotypes

Razi

Bae

Muneer

2021

IJMS

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As climate changes increase, drought stress is becoming a problem for all major horticultural crops; among them is okra (Abelmoschus esculentus). Despite its superior resilience to heat stress and high nutritional content, it is still underutilized in contrast to other vegetable crops. Moreover, the drought-resistant and drought-sensitive genotypes of okra are also not well known and require further exploration to improve their productivity. To investigate this in more detail, we performed comparative physiological and large-scale chloroplast proteomics on drought-stressed genotypes of okra. We evaluated four major genotypes of okra, viz., NS7774, NS7772, Green Gold, and OH3312 for drought resilient rootstock. The physiological modulations demonstrated a significant change by 50–76% in biomass, net-photosynthetic machinery, water transport, and absorption both in early and late stages of drought stress compared to well-watered crops in all genotypes. Maximum oxidative damage due to drought stress was observed for the genotypes NS7772, Green Gold and OH3312 as depicted by H2O2 and O2− determination. Greater oxidative stress was correlated to lesser antioxidant activity and expression of antioxidant enzymes, such as catalase and ascorbate peroxidase under stress in okra genotypes. The overall photosynthetic pigments, such as total chlorophyll, and total carotenoid content, were also decreased, and stomatal guard cells were disrupted and appeared closed compared to the control for the above three mentioned genotypes, except NS7774. A subsequent tissue-specific proteome analysis of chloroplasts and thylakoids analyzed by BN-PAGE (blue native polyacrylamide gel electrophoresis) revealed either over or under expression of specific proteins, such as ATPase, PSI, PSII core dimer, PSII monomer and ATP synthase. The expression of multiprotein complex proteins, including PSII-core dimer and PSII-core monomer, was slightly higher for the genotype NS7774 when compared to three other genotypes for both 5 and 10 days of drought stress. Further identification of specific proteins obtained in second dimension BN-PAGE provided descriptive detail of seven proteins involved in drought resistance across all genotypes. The identified proteins are majorly involved in photosynthesis under drought stress, suggesting NS7774 as a drought tolerant genotype. Further, the proteomic results were confirmed using Immunoblot by selecting specific protein such as PsaA. Overall, from our physiological modulations and chloroplast proteomics in all genotypes, we summarized NS7774 as a resilient rootstock and the other three genotypes (NS7772, OH3312, and Green Gold) as sensitive ones.

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Elucidation of stage specific physiological sensitivity of okra to drought stress through leaf gas exchange, spectral indices, growth and yield parameters

Cited by 35 publications

References 51 publications

Screening of Onion (Allium cepa L.) Genotypes for Drought Tolerance Using Physiological and Yield Based Indices Through Multivariate Analysis

Screening of Onion (Allium cepa L.) Genotypes for Drought Tolerance Using Physiological and Yield Based Indices Through Multivariate Analysis

Biochar and Arbuscular mycorrhizal fungi mediated enhanced drought tolerance in Okra (Abelmoschus esculentus) plant growth, root morphological traits and physiological properties

Target-Based Physiological Modulations and Chloroplast Proteome Reveals a Drought Resilient Rootstock in Okra (Abelmoschus esculentus) Genotypes

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