Elucidation of potentially virulent factors of Candida albicans during serum adaptation by using quantitative time-course proteomics

Aoki, Wataru; Tatsukami, Yohei; Kitahara, Nao; Matsui, Kazuma; Morisaka, Hironobu; Kuroda, Kouichi; Ueda, Mitsuyoshi

doi:10.1016/j.jprot.2013.07.031

Cited by 17 publications

(20 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the FBS series, only 2 (Sod5 and Blp1) of the 1,024 identified proteins were clustered in Group 1, and 4 proteins (Ece1, Ucf1, Stf2 and Hgt1) were clustered in Group 2. Other proteins in the FBS series showed moderate abundance changes [19]. Based on these results, we suggest that C. albicans employed the following adaptation strategy: first, C. albicans tuned its proteome to adapt to a new environment, in which most proteins were upregulated 2-fold higher than has been suggested by previous studies [23].…”

Section: Quantitative Time-course Proteomicssupporting

confidence: 52%

See 1 more Smart Citation

Combining Proteomic Strategies and Molecular Display Technology for Development of Vaccines against Candida albicans

Karasaki¹

2014

J Proteomics Bioinform

View full text Add to dashboard Cite

Section: Quantitative Time-course Proteomicssupporting

confidence: 52%

“…Aoki et al [15] has also reported a quantitative time-course proteomics study of C. albicans during the early stages of adaptation to serum (0-180 min) [19]. Quantitative time-course proteome analysis is a promising approach.…”

Section: Quantitative Time-course Proteomicsmentioning

confidence: 99%

Combining Proteomic Strategies and Molecular Display Technology for Development of Vaccines against Candida albicans

Karasaki¹

2014

J Proteomics Bioinform

View full text Add to dashboard Cite

“…The AMP may also protect normal microflora, resulting in enhanced safety. Conjunction of additional selective domains that target other virulent attribute of C. albicans may further increase specificity and activity [162,163,164,165]. …”

Section: Design Of Molecular-targeted Ampsmentioning

confidence: 99%

Characterization of Antimicrobial Peptides toward the Development of Novel Antibiotics

2013

Self Cite

View full text Add to dashboard Cite

Antimicrobial agents have eradicated many infectious diseases and significantly improved our living environment. However, abuse of antimicrobial agents has accelerated the emergence of multidrug-resistant microorganisms, and there is an urgent need for novel antibiotics. Antimicrobial peptides (AMPs) have attracted attention as a novel class of antimicrobial agents because AMPs efficiently kill a wide range of species, including bacteria, fungi, and viruses, via a novel mechanism of action. In addition, they are effective against pathogens that are resistant to almost all conventional antibiotics. AMPs have promising properties; they directly disrupt the functions of cellular membranes and nucleic acids, and the rate of appearance of AMP-resistant strains is very low. However, as pharmaceuticals, AMPs exhibit unfavorable properties, such as instability, hemolytic activity, high cost of production, salt sensitivity, and a broad spectrum of activity. Therefore, it is vital to improve these properties to develop novel AMP treatments. Here, we have reviewed the basic biochemical properties of AMPs and the recent strategies used to modulate these properties of AMPs to enhance their safety.

show abstract

“…Collected samples were frozen quickly using liquid nitrogen and preserved at −80°C until use. Proteins were extracted as described previously with minor modifications (Aoki et al 2013 ). Briefly, each sample was centrifuged for 5 min at 3,000× g , and the resulting cell pellets were suspended in 400 μl of lysis buffer [4% w/v 3-(3-cholamidepropyl)dimethylammonio-1-propanesulfonate, 1% w / v dithiothreitol, 1% v / v protease inhibitor cocktail for mammalian and yeast cells, 7 M urea, and 2 M thiourea in 20 mM Tris-HCl, pH 7.8].…”

Section: Methodsmentioning

confidence: 99%

Description of the interaction between Candida albicans and macrophages by mixed and quantitative proteome analysis without isolation

et al. 2015

Self Cite

View full text Add to dashboard Cite

Candida albicans is an opportunistic pathogen that causes fatal diseases in immunocompromised hosts. Host resistance against C. albicans relies on ingestion of the pathogen by macrophages. Analysis of the escaping behavior of C. albicans from macrophages is required to understand the onset of systemic candidiasis. In this study, native interactions of C. albicans with macrophages were investigated by proteome analysis using high efficiency of long monolithic silica capillary column. Using this system, we developed a method of “mixed and quantitative proteome analysis” in which C. albicans and macrophages were simultaneously analyzed by nanoLC–MS/MS without the need to isolate the two individual living cells. Two hundred twenty-seven proteins from C. albicans and five proteins from macrophages were identified as candidate interaction-specific molecules. C. albicans seemed to produce glucose through a β-oxidation pathway, a glyoxylate cycle, and gluconeogenesis for escape from macrophages. Up-regulation of stress-related and candidate pathogenic proteins in C. albicans indicated how C. albicans endured the harsh environment inside the macrophages. Down-regulation of apoptosis-associated protein NOA1- and chaperone HSPA1A-syntheses in macrophage indicated that C. albicans was able to escape from macrophages in part by suppressing the production of these macrophage proteins.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-015-0127-2) contains supplementary material, which is available to authorized users.

show abstract

Elucidation of potentially virulent factors of Candida albicans during serum adaptation by using quantitative time-course proteomics

Cited by 17 publications

References 62 publications

Combining Proteomic Strategies and Molecular Display Technology for Development of Vaccines against Candida albicans

Combining Proteomic Strategies and Molecular Display Technology for Development of Vaccines against Candida albicans

Characterization of Antimicrobial Peptides toward the Development of Novel Antibiotics

Description of the interaction between Candida albicans and macrophages by mixed and quantitative proteome analysis without isolation

Contact Info

Product

Resources

About