Elucidation of metabolic responses in mud crab Scylla paramamosain challenged to WSSV infection by integration of metabolomics and transcriptomics

Kong, Tongtong; Ren, Xin; Lin, Shanmeng; Li, Shengkang; Gong, Yi

doi:10.1016/j.dci.2020.103799

Cited by 10 publications

(8 citation statements)

References 41 publications

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“…Therefore, viral infections can cause metabolic disorders in host cells. 1,2 As previously reported, virus replication is associated with enhanced cellular metabolism in its host because it depends on its host for the synthesis of viral genomes and viral proteins. 3 In humans, hepatitis C virus (HCV) induces early interference of glycolysis, the pentose phosphate pathway, and the citric acid cycle, which is conducive to the biosynthetic activity of the host to support virus replication and reproduction.…”

Section: Introductionmentioning

confidence: 92%

miRNA in food simultaneously controls animal viral disease and human tumorigenesis

Cui¹,

Wan²,

Zhang³

2021

Molecular Therapy - Nucleic Acids

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During virus infection in animals, the virus completes its life cycle in a host cell. A virus infection results in the metabolic deregulation of its host and leads to metabolic disorders, ultimately paving the way for cancer progression. Because metabolic disorders in virus infections occurring in animal are similar to metabolic disorders in human tumorigenesis, animal antiviral microRNAs (miRNAs), which maintain the metabolic homeostasis of animal cells, in essence, may have anti-tumor activity in humans. However, that issue has not been investigated. In this study, shrimp miR-34, a potential antiviral miRNA of shrimp against white spot syndrome virus (WSSV) infection, was identified. Overexpression of shrimp miR-34 in shrimp fed bacteria expressing miR-34 suppressed WSSV infection by targeting the viral wsv330 and wsv359 genes. Furthermore, the expression of shrimp miR-34 in mice fed miR-34-overexpressing shrimp suppressed breast cancer progression by targeting human CCND1, CDK6, CCNE2, E2F3, FOSL1, and MET genes. Therefore, our study suggests that the miRNAs in food could be an effective strategy for synchronously controlling viral diseases of economic animals and cancers in humans.

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Section: Introductionmentioning

confidence: 92%

miRNA in food simultaneously controls animal viral disease and human tumorigenesis

Cui¹,

Wan²,

Zhang³

2021

Molecular Therapy - Nucleic Acids

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“…In addition to bacteria, viral outbreaks also cause severe adverse effects on hosts, including mortality, and lead to major economic losses 150 . For example, since its discovery in the 1990s, white spot syndrome virus (WSSV) has been known to infect a variety of hosts, including portunid crabs, and is transmittable via various routes, such as injection, feeding, and co‐inhabitation with infected individuals 151,152 . During early‐stage infection, WSSV disrupts several immune‐related metabolic pathways, including purine metabolism and taurine and hypotaurine metabolism, at the metabolic and transcriptional levels.…”

Section: Functional Categories Of Transcriptomics In Portunid Crabsmentioning

confidence: 99%

“…During early‐stage infection, WSSV disrupts several immune‐related metabolic pathways, including purine metabolism and taurine and hypotaurine metabolism, at the metabolic and transcriptional levels. In contrast, cysteine metabolism, methionine metabolism, and unsaturated fatty acid biosynthesis are dysregulated during late‐stage WSSV infection 152 . Based on the target genes of differentially expressed miRNAs pre‐ and post‐infection, WSSV infection affects the immunological pathways of portunid crabs, including phagocytosis, melanism, and apoptosis.…”

Section: Functional Categories Of Transcriptomics In Portunid Crabsmentioning

confidence: 99%

“…Multi‐omics integrates two or more omic technologies such as the genome, transcriptome, proteome, epigenome, metabolome, and microbiome to elucidate, at multiple levels, a specific biological process 200 . Several studies incorporated other omics into their research to complement the transcriptomic data of portunid crabs, specifically in the field of immune 152 and stress responses 174 . In addition, multi‐omics has been shown to be useful in understanding other biological processes in invertebrates, including sex differentiation and maturation, 201 growth, 202 and nutrition 203 …”

Section: Future Portunid Transcriptomic Research Directionsmentioning

confidence: 99%

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Transcriptomics in advancing portunid aquaculture: A systematic review

Waiho

Ikhwanuddin

Afiqah‐Aleng

et al. 2022

Reviews in Aquaculture

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The rapid advancement of next-generation sequencing technologies has promoted the use of transcriptomics in non-model organisms, including species in the aquaculture sector. Compared to that of other crustacean species, portunid crab aquaculture is impeded by the insufficient knowledge of their biological and physiological processes. This systematic review summarised the advances in transcriptomics in cultured portunid crabs using a systematic literature review methodology. The filtered transcriptome dataset comprised 66 articles from four genera: Scylla, Portunus, Cal-

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“…The recent development of effective “omic” data analysis and bioinformatic tools enables the study of the biological responses of aquatic animal cells or organs to environmental stress and disease infection through the analysis of transcriptomes, metabolomes, and proteomes [ 15 , 16 ]. Among them, transcriptome analysis can identify stress-induced genes and provides important information on gene expression levels, thus enabling the visualization of the expression patterns of key genes [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Integrative Analysis of Transcriptome and Metabolome Reveals Molecular Responses in Eriocheir sinensis with Hepatopancreatic Necrosis Disease

Zhan

Wen

Zhu

et al. 2022

Biology

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Hepatopancreatic necrosis disease (HPND) is a highly lethal disease that first emerged in 2015 in Jiangsu Province, China. So far, most researchers believe that this disease is caused by abiotic factors. However, its true pathogenic mechanism remains unknown. In this study, the effects of HPND on the metabolism and other biological indicators of the Chinese mitten crab (Eriocheir sinensis) were evaluated by integrating transcriptomics and metabolomics. Our findings demonstrate that the innate immunity, antioxidant activity, detoxification ability, and nervous system of the diseased crabs were affected. Additionally, metabolic pathways such as lipid metabolism, nucleotide metabolism, and protein metabolism were dysregulated, and energy production was slightly increased. Moreover, the IL-17 signaling pathway was activated and high levels of autophagy and apoptosis occurred in diseased crabs, which may be related to hepatopancreas damage. The abnormal mitochondrial function and possible anaerobic metabolism observed in our study suggested that functional hypoxia may be involved in HPND progression. Furthermore, the activities of carboxylesterase and acetylcholinesterase were significantly inhibited, indicating that the diseased crabs were likely stressed by pesticides such as pyrethroids. Collectively, our findings provide new insights into the molecular mechanisms altered in diseased crabs, as well as the etiology and pathogenic mechanisms of HPND.

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Elucidation of metabolic responses in mud crab Scylla paramamosain challenged to WSSV infection by integration of metabolomics and transcriptomics

Cited by 10 publications

References 41 publications

miRNA in food simultaneously controls animal viral disease and human tumorigenesis

miRNA in food simultaneously controls animal viral disease and human tumorigenesis

Transcriptomics in advancing portunid aquaculture: A systematic review

Integrative Analysis of Transcriptome and Metabolome Reveals Molecular Responses in Eriocheir sinensis with Hepatopancreatic Necrosis Disease

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