Elucidation of flavonoids from Carissa congesta, Polyalthia longifolia, and Benincasa hispida plant extracts by hyphenated technique of liquid chromatography-mass spectroscopy

Abstract

Background:Carissa congesta (CC), Polyalthia longifolia (PL), and Benincasa hispida (BH) are economically important plants.Objective:Current research encompasses identification of quercetin and rutin and their analogues by liquid chromatography-mass spectroscopy (LC-MS) from the selected plant species.Materials and Methods:Fresh roots, leaves, and seeds of CC, PL, and BH plants respectively were shade-dried followed by extraction and elucidation of rutin and quercetin by LC-MS.Results:Structural elucidation of CC, PL, and BH extracts revealed the presence of flavonoids such as quercetin (m/z 301) and rutin (m/z 610) as the parent ions along with presence of close analogues such as quercetin-O-hexoside, Vicenin 2, quercetin-3-O-xyloside/arabinoside, and quercetin-3-O-glucoside were identified as fragments.Conclusions:Thus, CC, PL, and BH extracts revealed the presence of flavonoids belonging to the class of flavonols such as rutin and quercetin.SUMMARY Quercetin and rutin were identified from CC roots, PL leaves and BH seeds by liquid chromatography-mass spectroscopy.Quercetin was characterized at (m/z 301) and rutin (m/z 610) as the parent ion peaks.Analogues such as quercetin-O-hexoside, Vicenin 2 and quercetin-3-O-glucoside were identified as fragments.

Exploring the Potential Use of Hylocereus polyrhizus Peels as a Source of Cosmeceutical Sunscreen Agent for Its Antioxidant and Photoprotective Properties

“…Due to the lack of standards, only rutin was validated using standard. As illustrated in Figure 4, rutin was spotted at m/z 609.14 and gave rise to intense ions at m/z 301.03 corresponding to the loss of the rutinose unit while fragment ions at m/z 594.15 indicate the presence of oxygen ion, in accordance to previous literatures [50][51][52]. In another research that was conducted to optimize extraction conditions for higher total phenolic flavonoids recovery and to determine in vitro antioxidant activities of defatted H. polyrhizus seed extract (DPSE) using response surface methodology, UPLC-QTOF/ MS analysis revealed the presence of rutin at the retention time 8.38 and m/z peak 609.14 which is in a good agreement with the findings of this study.…”

Phytochemicals from Polyalthia Species: Potential and Implication on Anti-Oxidant, Anti-Inflammatory, Anti-Cancer, and Chemoprevention Activities

“…Other major bioactive phytochemicals in Polyalthia species are flavonoids, lignans, sterols, organic acids, etc. [27,[38][39][40][41][42]. In fact, clerodane-type diterpenes may be one of the most well-studied and enrichedcompound in Polyalthia species, and the pharmacological and physiological functions of 16-hydroxycleroda-3,13-dien-15,16-olide (36 and/or 38, abbreviated CD or HCD in literatures) have been studied by several groups [24,[43][44][45][46][47].…”

A Chinese Herbal Formula Suppresses Colorectal Cancer Migration and Vasculogenic Mimicry Through ROS/HIF-1α/MMP2 Pathway in Hypoxic Microenvironment

“…P value of ≤0.05 was considered statistically significant. 9,10-dimethoxypterocarpan, luteolin, emodin-3-methyl ether, 5-O-caffeoyl quinic acid methyl ester, 3-hydroxy-9,10dimethoxyptercarpan, curcumenol, atractylenolide III, 2atractylenolide, isorhamnetin, astraisoflavanglucoside-6″-Omalonate, ononin, 3b-O-acetyl ursolic acid, 3b-O-acetyl oleanolic acid, and seco isolariciresinol 9-O -b -Dglucopyranoside by comparing the fragment ions with those of characteristic components of the herbal preparation reported in literatures (Lin et al, 2000;Doshi et al, 2016;Sun et al, 2017;Zhu et al, 2018) (Table 3).…”