Elucidation for the pharmacological effects and mechanism of Shen Bai formula in treating myocardial injury based on energy metabolism and serum metabolomic approaches

Song, Hongwei; Ren, Junling; Yang, Le; Sun, Hui; Yan, Guangli; Han, Ying; Wang, Xijun

doi:10.1016/j.jep.2023.117670

Cited by 2 publications

(1 citation statement)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rat model of acute MI prepared with Pit is similar to the pathogenesis of clinical-variant angina pectoris, and has been widely used for screening anti-myocardial ischemia drugs and in the study of related mechanisms of action [25][26][27]. MI leads to myocardial cell damage and changes in membrane permeability, resulting in a large number of enzymes, proteins, and their decomposition products being released into the blood, among which CK, LDH, AST, and HBDH are important markers by which to measure myocardial damage [28][29][30]. SOD is an active oxygen-scavenging agent that can convert superoxide anions into hydrogen peroxide in the body and then convert it into water through the action of other enzymes, which can reflect the degree of oxidative stress in the body [31].…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetic–Pharmacodynamic Correlation Analysis of Rhodiola crenulata in Rats with Myocardial Ischemia

Jia,

Zou,

Xie

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

The pharmacokinetics (PK) of Rhodiola crenulata in rats were studied, and pharmacokinetic–pharmacodynamic (PK-PD) correlation analysis was performed to elucidate their time–concentration–effect relationship. The myocardial ischemia model was made with pituitrin. Rats were divided into sham operation, sham operation administration, model, and model administration groups (SG, SDG, MG, and MDG, respectively; n = 6). Blood was collected from the fundus venous plexus at different time points after oral administration. The HPLC-QQQ-MS/MS method was established for the quantification of five components of Rhodiola crenulata. CK, HBDH, SOD, LDH, and AST at different time points were detected via an automatic biochemical analyzer. DAS software was used to analyze PK parameters and PK-PD correlation. The myocardial ischemia model was established successfully. There were significant differences in the PK parameters (AUC0–, AUC0–∞, Cmax) in MDG when compared with SDG. Two PD indicators, CK and HBDH, conforming to the sigmoid-Emax model, had high correlation with the five components, which indicated a delay in the pharmacological effect relative to the drug concentration in plasma. The difference in the PK parameters between modeled and normal rats was studied, and the time–concentration–effect of composition and effect indicators were investigated. This study can provide reference for the rational clinical application of Rhodiola crenulata and for related studies of other anti-myocardial ischemia drugs.

show abstract