Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures

Pabit, Suzette A.; Chen, Yen-Lin; Usher, Grace A.; Cook, Erik C.; Pollack, Lois; Showalter, Scott A.

doi:10.1016/j.bpj.2020.10.038

Cited by 4 publications

(1 citation statement)

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“…Pabit et al. studied the effect of DGCR8 binding to pri‐miRNA‐16‐1 on the RNA structure [112] by CV‐SAXS [64a] (see Section 2.3. and Table 1). Upon complex formation, the P ( r ) curve shows a population shift to larger distances, indicative of an RNA structure that deviates from a straight dsRNA, achievable by bending the RNA through the protein.…”

Section: Applications Of Saxs In Rna Biologymentioning

confidence: 99%

Advances, Applications, and Perspectives in Small‐Angle X‐ray Scattering of RNA

Tants

Schlundt

2023

ChemBioChem

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RNAs exhibit a plethora of functions far beyond transmitting genetic information. Often, RNA functions are entailed in their structure, be it as a regulatory switch, protein binding site, or providing catalytic activity. Structural information is a prerequisite for a full understanding of RNA‐regulatory mechanisms. Owing to the inherent dynamics, size, and instability of RNA, its structure determination remains challenging. Methods such as NMR spectroscopy, X‐ray crystallography, and cryo‐electron microscopy can provide high‐resolution structures; however, their limitations make structure determination, even for small RNAs, cumbersome, if at all possible. Although at a low resolution, small‐angle X‐ray scattering (SAXS) has proven valuable in advancing structure determination of RNAs as a complementary method, which is also applicable to large‐sized RNAs. Here, we review the technological and methodological advancements of RNA SAXS. We provide examples of the powerful inclusion of SAXS in structural biology and discuss possible future applications to large RNAs.

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Section: Applications Of Saxs In Rna Biologymentioning

confidence: 99%