Elucidating the role of extracellular polymeric substances (EPS) in dewaterability of fecal sludge from onsite sanitation systems, and changes during anaerobic storage.

Genomic and functional evaluation of exopolysaccharide produced by Liquorilactobacillus mali t6-52: technological implications

“…In our study, the efficacy of EPS became more pronounced after an extended 3-month storage period. This intriguing phenomenon could be attributed to several factors, including the maturation of the EPS matrix, gradual interaction with bacterial cells, and concentrationdependent effects [60][61][62][63]. The extended storage period likely allows for the optimization of the EPS matrix, leading to a more effective shield against freeze-thaw stresses.…”

Urban Sanitation: New Terminology for Globally Relevant Solutions?

“…A treatment plant may include stages of sludge treatment, including thickening, digestion, dewatering, and hygienization, depending on the type of wastewater treatment process and the destination of the sludge . Scientific evidence about differences between blackwater that has been stored in containment in comparison to conventional wastewater sludges is mounting, including dewatering performance, ,,,, protein-like fractions of extracellular polymeric substances (EPS), , particle size distribution, , fibers and lipids, , rheological properties, , and microbial communities …”

“…37 However, stratification occurs with continual inputs of "fresh" excreta to containments, and storage is not analogous to anaerobic digestion that is process controlled at treatment facilities to optimize microbial degradation, 38 . 39 Changes to wastewater during passive anaerobic storage have been observed to level off after 1 week, 40,41 and average biochemical oxygen demand (BOD 5 ) concentrations of almost 10 000 mg/L have been observed in the bottom regions of pit latrines. 42 Wastewater in containment is also different from what is termed "sludges" in sewer to treatment systems.…”

Mechanism Involved in Polyvinyl Chloride Nanoplastics Induced Anaerobic Granular Sludge Disintegration: Microbial Interaction Energy, EPS Molecular Structure, and Metabolism Functions

“…The components of EPS impact the surface charge and hydrophobicity of microbial flocs, thereby affecting bacterial aggregation . EPS are important microbial products and the organic macromolecules in EPS are produced by biosynthetic polymerization of specific building blocks. , It has been reported that 20 different amino acids, including arginine and alanine, involved in the synthesis of bacterial proteins, and monosaccharides were utilized to synthesize microbial polysaccharides via the Embden–Meyerhoff–Parnas gluconeogenesis (EMP) pathway . Phosphatidylethanolamine (PtE) and phosphatidylcholine (PtC) were the primary components of extracellular PLP. , As can be seen, microbial metabolism significantly determines the EPS level and chemical composition as well as microbial aggregation potential and the granule spatial structure.…”

“…24 EPS are important microbial products and the organic macromolecules in EPS are produced by biosynthetic polymerization of specific building blocks. 25,26 It has been reported that 20 different amino acids, including arginine and alanine, involved in the synthesis of bacterial proteins, 27 and monosaccharides were utilized to synthesize microbial polysaccharides via the Embden−Meyerhoff−Parnas gluconeogenesis (EMP) pathway. 28 Phosphatidylethanolamine (PtE) and phosphatidylcholine (PtC) were the primary components of extracellular PLP.…”