Elucidating the Physio-Morphological and Biochemical Responses towards PEG-Induced Drought Stress in Finger Millet Genotypes

Jamra, Gautam; Shah, Pallavi; Agarwal, Aparna; Sharma, Divya Bhanu; Kumar, Anil

doi:10.20546/ijcmas.2020.907.193

Cited by 2 publications

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References 40 publications

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“…Our previous studies have established that-a) GP45 is a drought-tolerant genotype (Jamra et al 2020) and b) CaM genes are highly expressed in GP45 (Kumar et al 2014). So we asked if the EcCaM, we were investigating, in this study had a role in drought stress.…”

Section: Resultsmentioning

confidence: 99%

“…So we asked if the EcCaM, we were investigating, in this study had a role in drought stress. Therefore, we performed qRT-PCR to analyse the expression pro le of EcCaM under 20% PEG induced drought stress condition we had previously used (Jamra et al 2020) and salinity stress (200mM NaCl) with the time frame of 3 and 6 hours after treatment. EcCaM transcript was increased within 3 hrs after exposure of the seedlings to PEG medium and reached a maximum to 6 hrs (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…It was reported that EcCaM transcripts were expressed strongly in developing spikes (Singh et al 2014) and the protein is localized more in embryo and aleurone layer of grains of high Ca 2+ nger millet genotype, GP-45 (Kumar et al 2014). It was hypothesized that higher expression and accumulation of EcCaM played a role in the drought tolerance of the GP-45 genotype (Jamra et al 2020). So in the present study, we elucidate the in planta functioning of this nger millet calmodulin, EcCaM.…”

Section: Introductionmentioning

confidence: 88%

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Ectopic expression of finger millet calmodulin confers drought and salinity tolerance in Arabidopsis thaliana

et al. 2021

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Drought and salinity are major environmental stresses which affect crop productivity and therefore are major hindrance in feeding growing population world-wide. Calcium (Ca 2+ ) signalling plays a crucial role during the plant's response to these stress stimuli. Calmodulin (CaM), a crucial Ca 2+ sensor, is involved in transducing the signal downstream in various physiological, developmental and stress responses by modulating a plethora of target proteins. The role of CaM has been well established in the model plant Arabidopsis thaliana for regulating various developmental processes, stress signalling and ion transport. In the current study, we investigate the CaM of Eleusine coracana (common name nger millet, known especially for its drought tolerance and superior Ca 2+ content). In-silico analysis showed that Eleucine calmodulin (EcCaM) has greater similarity to rice CaM as compared to Arabidopsis CaM due to the presence of highly conserved four EF-hand domains. To decipher the in planta function of EcCaM, we have adopted the gain-of-function approach by generating the 35S::EcCaM over-expression transgenic in Arabidopsis. Overexpression of EcCaM in Arabidopsis makes the plant tolerant to polyethylene glycol (PEG) induced drought and salt stress (NaCl) as demonstrated by post-germination based phenotypic assay, ion leakage, MDA and proline estimation, ROS detection under stressed and normal conditions. Moreover, EcCaM-overexpression leads to hypersensitivity towards exogenously applied ABA at the seed germination stage. These ndings reveal that EcCaM mediates tolerance to drought and salinity stress. Also, our results indicate that EcCaM is involved in modulating ABA signalling. Summarizing our results, we report for the rst time that EcCaM is involved in modulating plants response to stress and this information can be used for the generation of future-ready crops that can tolerate a wide range of abiotic stresses.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 88%

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