Elucidating the effect of anti-biofilm activity of bioactive compounds extracted from plants

Lahiri, Dibyajit; Dash, Satyabhusan; Dutta, Rachayeeta; Nag, Moupriya

doi:10.1007/s12038-019-9868-4

Cited by 83 publications

(55 citation statements)

References 150 publications

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“…This observation is contrary to a study which found that some P. aeruginosa biofilm cells exhibited altered morphology as a result of exposure to CT [35]. The differences in these observations corroborates the views that polyphenols from different plant sources [12] differ in their biological activity [66] at varying concentrations [67].…”

Section: Biofilm Prevention Using Phlorotanninssupporting

confidence: 59%

“…The potential of biofilms to resist antimicrobial treatment is due to a protective outer layer, the exopolysaccharide matrix and the multiple bacterial layers of biofilms which protect embedded cells against disinfectants and other antimicrobial agents [11,12]. The ability to chelate cationic antimicrobials as a result of the presence of extracellular DNA within the biofilm matrix [13] and to concentrate enzymes such as beta-lactamases, increases the resistance of biofilms to antimicrobials [14].…”

Section: Introductionmentioning

confidence: 99%

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Activity of Bacteriophage and Complex Tannins against Biofilm-Forming Shiga Toxin-Producing Escherichia coli from Canada and South Africa

Bumunang

Ateba

Stanford³

et al. 2020

Antibiotics

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Bacteriophages, natural killers of bacteria, and plant secondary metabolites, such as condensed tannins, are potential agents for the control of foodborne pathogens. The first objective of this study evaluated the efficacy of a bacteriophage SA21RB in reducing pre-formed biofilms on stainless-steel produced by two Shiga toxin-producing Escherichia coli (STEC) strains, one from South Africa and the other from Canada. The second objective examined the anti-bacterial and anti-biofilm activity of condensed tannin (CT) from purple prairie clover and phlorotannins (PT) from brown seaweed against these strains. For 24-h-old biofilms, (O113:H21; 6.2 log10 colony-forming units per square centimeter (CFU/cm2) and O154:H10; 5.4 log10 CFU/cm2), 3 h of exposure to phage (1013 plaque-forming units per milliliter (PFU/mL)) reduced (p ≤ 0.05) the number of viable cells attached to stainless-steel coupons by 2.5 and 2.1 log10 CFU/cm2 for O113:H21 and O154:H10, respectively. However, as biofilms matured, the ability of phage to control biofilm formation declined. In biofilms formed for 72 h (O113:H21; 5.4 log10 CFU/cm2 and O154:H10; 7 log10 CFU/cm2), reductions after the same duration of phage treatment were only 0.9 and 1.3 log10 CFU/cm2 for O113:H21 and O154:H10, respectively. Initial screening of CT and PT for anti-bacterial activity by a microplate assay indicated that both STEC strains were less sensitive (p ≤ 0.05) to CT than PT over a concentration range of 25–400 µg/mL. Based on the lower activity of CT (25–400 µg/mL), they were not further examined. Accordingly, PT (50 µg/mL) inhibited (p ≤ 0.05) biofilm formation for up to 24 h of incubation at 22 °C, but this inhibition progressively declined over 72 h for both O154:H10 and O113:H21. Scanning electron microscopy revealed that both SA21RB and PT eliminated 24 h biofilms, but that both strains were able to adhere and form biofilms on stainless-steel coupons at longer incubation times. These findings revealed that phage SA21RB is more effective at disrupting 24 than 72 h biofilms and that PT were able to inhibit biofilm formation of both E. coli O154:H10 and O113:H21 for up to 24 h.

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Section: Biofilm Prevention Using Phlorotanninssupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Activity of Bacteriophage and Complex Tannins against Biofilm-Forming Shiga Toxin-Producing Escherichia coli from Canada and South Africa

Bumunang

Ateba

Stanford³

et al. 2020

Antibiotics

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“…Additionally, local or systemic administered antibiotics are largely ineffective for peri-implant infections due to bacterial drug resistance, poor drug penetration, and suboptimal bioavailability at the site of infection [ 2 ]. Recent studies have demonstrated the effectiveness of plant secondary metabolites (phytochemicals) where bacterial resistance mechanisms, including multidrug resistance, make traditional therapy unsuccessful, even in the control of biofilms [ 8 , 9 ]. In this respect, phytochemicals exert their antibacterial activity through different mechanisms of action including bacterial membrane damage, inhibition of enzymes and toxins, and bacterial biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

pH-Responsive Resveratrol-Loaded Electrospun Membranes for the Prevention of Implant-Associated Infections

Bonadies

Cristo²,

Valentino³

et al. 2020

Nanomaterials

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To date, the implant-associated infections represent a worldwide challenge for the recently reported bacterial drug resistance that can lead to the inefficacy or low efficacy of conventional antibiotic therapies. Plant polyphenolic compounds, including resveratrol (RSV), are increasingly gaining consensus as valid and effective alternatives to antibiotics limiting antibiotic resistance. In this study, electrospun polylactic acid (PLA) membranes loaded with different concentrations of RSV are synthesized and characterized in their chemical, morphological, and release features. The obtained data show that the RSV release rate from the PLA-membranes is remarkably higher in acidic conditions than at neutral pH. In addition, a change in pH from neutral to slightly acidic triggers a significant increase in the RSV release. This behavior indicates that the PLA-RSV membranes can act as drug reservoir when the environmental pH is neutral, starting to release the bioactive molecules when the pH decreases, as in presence of oral bacterial infection. Indeed, our results demonstrate that PLA-RSV2 displays a significant antibacterial and antibiofilm activity against two bacterial strains, Pseudomonas aeruginosa PAO1, and Streptococcus mutans, responsible for both acute and chronic infections in humans, thus representing a promising solution for the prevention of the implant-associated infections.

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“…It also established the fact of inhibition of the growth of E. coli, isolated from the urogenital tract when affected by berry extract of V. vitis-idaea (Wojnicz, 2012). The antibacterial activity of lingonberry leaf extract against E. coli was ascertained (Lahiri et al, 2019). The anti-adhesive properties were shown for the use of proto-anthocyanidines of Vaccinium macrocarpon Aiton berry (Huber et al, 2003).…”

Section: Discussionmentioning

confidence: 87%

Antibiofilm forming, antimicrobial activity and some biochemical properties of Vaccinium vitis idaea leaf and berry extracts on Staphylococcus aureus

et al. 2020

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Infections caused by Staphylococcus genus bacteria remain a relevant problem due to the high percentage of antibiotic-resistant biofilm-forming strains of isolates of this genus. Herbs are a promising source for many biologically active compounds with antimicrobial properties. The aim of the research was to study the antimicrobial and antibiofilm formation activity of berry and leaf extracts of Vaccinium vitis-idaea L. upon clinical isolates of S. aureus, and the main biochemical properties of these extracts. For the purpose of analysis, we used S. aureus isolated from the mouth cavities and pharynx of human patients suffering from inflammatory diseases. The plants for the study were gathered in Pylypets, Mizhhiria rayon, Zakarpatska oblast (Transcarpathia). From Vaccinium vitis-idaea L., leaf and berry extracts were produced. To determine the chemical properties of the extracts, the following constituents were investigated: total tannin, flavonoids, total phenols, anthocyanins (by spectrophotometric method), and the total amount of vitamin C in berry extract (chromatographically). The antimicrobial activity was studied by diffusion-into-agar method and determination of minimum inhibitory concentrations. The antibiofilm activity of the extracts was tested in standard 96-well microtitration plates. The main chemical composition of ethyl extracts of Vaccinium vitis-idaea L. berries and leaves was identified. The level of tannins in leaf extracts was established to be higher than in fruit extracts (3.50% and 0.26% per 100 g of extract, respectively). It was shown that extracts of V. vitis-idaea berries and leaves demonstrate high antimicrobial activity against clinical isolates of S. aureus. Further it was established that leaf extracts had high ability to destroy the bacterial biofilm of S. aureus. Leaf extracts were also able to destroy the formed biofilm. Even in the 0.01% concentration, leaf extract inhibited the formation of the biofilm by 69.9% and caused the destruction of the formed biofilm by 62.5%. Thereby, the obtained results show good prospects for the use of V. vitis-idaea leaf extracts as an anti-staphylococcal remedy with antibiofilm forming properties.

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Elucidating the effect of anti-biofilm activity of bioactive compounds extracted from plants

Cited by 83 publications

References 150 publications

Activity of Bacteriophage and Complex Tannins against Biofilm-Forming Shiga Toxin-Producing Escherichia coli from Canada and South Africa

Activity of Bacteriophage and Complex Tannins against Biofilm-Forming Shiga Toxin-Producing Escherichia coli from Canada and South Africa

pH-Responsive Resveratrol-Loaded Electrospun Membranes for the Prevention of Implant-Associated Infections

Antibiofilm forming, antimicrobial activity and some biochemical properties of Vaccinium vitis idaea leaf and berry extracts on Staphylococcus aureus

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