Elucidating role of silk-gelatin bioink to recapitulate articular cartilage differentiation in 3D bioprinted constructs

Chawla, Smriti; Kumar, Aditi; Admane, Prasad; Bandyopadhyay, Amitabha; Ghosh, Sourabh

doi:10.1016/j.bprint.2017.05.001

Cited by 70 publications

(91 citation statements)

References 58 publications

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“…a) Cells formed strong attachment with silk‐gelatin bioink, degraded and remodeled pericellular matrix, b) cells expressed lamellipodia, filopodia to contract bioink matrix, c–e) cells migrate from embedded spheroids (arrows in c,d), probably triggered by HIF1α expression (red) and form new aggregates, e) immediately after printing cells were entrapped in randomly oriented manner, but (b) with time cells showed coordinated directional sensing and cellular alignment toward the 3D printed bioink filaments (Figure of ref. ).…”

Section: Biological Characterizationmentioning

confidence: 97%

“…There are two different approaches using 3D printing technology in tissue engineering. The first strategy is to develop acellular 3D porous scaffolds, and top‐seed cells post‐printing; while the second approach is used to create tissue equivalents by directly depositing cells (in the form of dispersed or aggregates) within the bioink, a process known as bioprinting . The intent of 3D bioprinting strategy is to create a tissue‐specific construct with 3D stacks/filaments of a hydrogel‐based matrix with explicit control over deposition of cell seeding modalities under strictly regulated microenvironment with gradients of growth factors, cytokines/chemokines and other biological moieties in optimal concentrations .…”

Section: Introductionmentioning

confidence: 99%

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Silk‐Based Bioinks for 3D Bioprinting

Chawla

Midha

Sharma

et al. 2018

Adv Healthcare Materials

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158

107

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3D bioprinting field is making remarkable progress; however, the development of critical sized engineered tissue construct is still a farfetched goal. Silk fibroin offers a promising choice for bioink material. Nature has imparted several unique structural features in silk protein to ensure spinnability by silkworms or spider. Researchers have modified the structure-property relationship by reverse engineering to further improve shear thinning behavior, high printability, cytocompatible gelation, and high structural fidelity. In this review, it is attempted to summarize the recent advancements made in the field of 3D bioprinting in context of two major sources of silk fibroin: silkworm silk and spider silk (native and recombinant). The challenges faced by current approaches in processing silk bioinks, cellular signaling pathways modulated by silk chemistry and secondary conformations, gaps in knowledge, and future directions acquired for pushing the field further toward clinic are further elaborated.

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Section: Biological Characterizationmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Silk‐Based Bioinks for 3D Bioprinting

Chawla

Midha

Sharma

et al. 2018

Adv Healthcare Materials

Self Cite

158

107

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“…Furthermore, SHM is also emerging as a powerful imaging tool in cartilage tissue engineering. Label-free in-situ detection of collagen expression, collagen types, and associated submicron level structural information could also be highly useful in optimizing the fabrication procedures of bioprinted regenerative articular cartilage [59].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Articular Cartilage by Second Harmonic Microscopy: Challenges and Opportunities

Kumar

2019

Front. Phys.

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Second harmonic microscopy (SHM) has emerged as a powerful non-destructive optical imaging modality that has high potential to perform the advanced structural characterization of intact articular cartilage. This article provides the current status, opportunities, and challenges of SHM for the use in cartilage evaluation. A novel perspective on SHM is being addressed that includes the reason, need and the importance of SHM in the assessment of articular cartilage. By taking the advantage of collagen specificity and high-resolution imaging, SHM can detect the structural and morphological changes in early stage cartilage damage that would otherwise be overlooked by standard clinical imaging methods such as radiography and arthroscopy. The current status of SHM for articular cartilage analysis and the major progress reported in recent literature are briefly described herein. We summarize the status of scientific efforts and the challenges that need to overcome before moving toward clinical applications of SHM in the context of cartilage evaluation.

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“…Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (QT00079247) was considered as housekeeping gene and 2D day 1 sample was taken as standard for all calculations. The analysis was carried out with the Rotor gene Q software using the 2 −(ΔΔc(t)) method (Chawla, Kumar, Admane, Bandyopadhyay, & Ghosh, 2017). The primers were optimized with respect of cells of goat origin (Chameettachal, Murab, Vaid, Midha, & Ghosh, 2017).…”

Section: Quantitative Real Time Polymerase Chain Reaction (Qrt-pcr)mentioning

confidence: 99%

Establishment of in vitro model of corneal scar pathophysiology

Chawla

Ghosh

2017

Journal Cellular Physiology

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Corneal scarring is the major source of permanent blindness worldwide. The complex pathophysiology of corneal scarring is not comprehensibly understood as it involves the interaction of a constellation of pro-fibrotic cytokines influencing several signaling pathways involved in corneal scar development. In the present study, an attempt has been made to generate a relatively simple in vitro corneal scar model using primary corneal keratocytes by exogenously providing an optimized dose of combination of cytokines (TGF-β1, IL-6, and IL-8) involved in scar formation in situ. Data obtained from gene and protein expression analysis depicted enhanced ECM production with discrete expression of myofibroblast specific markers. The protein-protein interactions associated these proteins to various pathways involved in wound healing, cellular migration, and cytoskeletal remodeling justifying high relevance to in vivo scar formation. Hence the developed model can be used to acquire understanding about corneal scar pathophysiology and thus might be useful for designing the treatment modalities and efficacies for controlling scar formation.

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Elucidating role of silk-gelatin bioink to recapitulate articular cartilage differentiation in 3D bioprinted constructs

Cited by 70 publications

References 58 publications

Silk‐Based Bioinks for 3D Bioprinting

Silk‐Based Bioinks for 3D Bioprinting

Assessment of Articular Cartilage by Second Harmonic Microscopy: Challenges and Opportunities

Establishment of in vitro model of corneal scar pathophysiology

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