Elucidating microbial community adaptation to anaerobic co-digestion of fats, oils, and grease and food waste

Inhibition of anaerobic digestion processes: Applications of molecular tools

“…For example, Methanosaeta remained the most abundant methanogen based on DNA-sequencing, but was strongly inhibited by high salt concentration according to RNA-based sequencing, corroborating performance observations. Similar observations have been reported during high FOG addition (Amha et al, 2017), providing further evidence supporting the need for RNA-based methods when evaluating AD inhibition. Recent developments in molecular methods have resulted in a greater understanding of inhibition in AD, as discussed in subsequent sections.…”

“…We aimed to draw on data from existing 16S rRNA gene sequence datasets to investigate how microbial communities vary across AD over a range of fatty acid concentrations. We collected 16S rRNA gene sequence data and metadata available from four different publicly available studies that included a total of 99 samples from bench-and full-scale ADs operated at mesophilic and thermophilic temperatures (Amha et al, 2017;Liu et al, 2016;Ma et al, 2015;Treu et al, 2016). These studies were selected based on sequence data and metadata availability and their comparison of LCFA and VFA inhibition.…”

Identification of parameters needed for optimal anaerobic co-digestion of chicken manure and corn stover

“…58 The presence of Clostridium and Syntrophomonas species has been previously reported to be an indicator of AD stability. 59,60 This is consistent with the high abundance of Clostridium and Syntrophomonas (p < 0.01) in HSS-AD communities, and the positive correlation between their abundance and cumulative methane production (p < 0.01, R 2 ¼ 0.78) ( Fig. 1A, and 4A).…”

Microbial rRNA gene expression and co‐occurrence profiles associate with biokinetics and elemental composition in full‐scale anaerobic digesters

“…A). Large deviations between archaeal RNA and DNA gene profiles for 16S rRNA were previously associated with stressful conditions during anaerobic digestion caused by high salinity (De Vrieze et al ., 2016b) and high LCFA levels (Amha et al ., ). If larger archaeal 16S rRNA and rRNA gene distances are indicative of stressful conditions, this would suggest that the full‐scale codigesters had higher stress levels than SS digesters.…”

“…Higher concentrations of Syntrophomonas have previously been correlated with higher degradation rates of LCFA in anaerobic digestion (Ziels et al ., , , ). Recently, 16S rRNA gene expression of Syntrophomonas was correlated with methane production from LCFA‐rich waste (Amha et al ., ). The presence and activity of SAO bacteria in the codigesters were associated with lower maximum rates of acetate consumption (Fig.…”