Elucidating comportment of the glutamate and ornithine pathway on proline accumulation in rice under different nitrogenous nutrition

Li, Y.-H.; Tian, Peng; Li, C.-Z.; Yu, Xiao-Zhang

doi:10.1007/s13762-021-03274-6

Cited by 7 publications

(11 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The proline content was determined according to Li et al [32] with ninhydrin. The absorbance of the red chromophore in the toluene fraction was read at 520 nm, and proline content was expressed as μg g −1 .…”

Section: Determination Of Proline Gaba P5c Methylglyoxal and 1-pyrrol...mentioning

confidence: 99%

Foliar application of procyanidins enhanced the biosynthesis of 2-acetyl-1-pyrroline in aromatic rice (Oryza sativa L.)

et al. 2022

View full text Add to dashboard Cite

Background Procyanidins is a polyphenolic compound with multiple properties. However, the application of exogenous procyanidins in crops has not been reported. Aromatic rice is a high-quality rice with a special aroma and popular with consumers. The 2-acetyl-1-pyrroline (2-AP) is a key compound of aromatic rice aroma. In the current study, aromatic rice plants were sprayed with procyanidins solutions at 0.25 (Pr0.25), 0.50 (Pr0.50), 1.00 (Pr1.00), 2.00 (Pr2.00) g L−1, respectively and treatment sprayed with distilled water was taken as control (CK). The effects of exogenous procyanidins on growth and 2-AP biosynthesis of aromatic rice plants were explored. Results Compared with CK, Pr1.00 and Pr2.00 treatments significantly increased 2-AP content by 16.67% and 37.68%, respectively. Higher proline, 1-pyrroline-5-carboxylic acid (P5C), 1-pyrroline, methylglyoxal contents, and lower γ- aminobutyric acid (GABA) content were recorded in Pr1.00 and Pr2.00 treatments than CK. Compared with CK, Pr1.00 and Pr2.00 treatments significantly improved the activities of P5CS and OAT and diminished the activity of BADH. Furthermore, compared with CK, Pr1.00 and Pr2.00 treatments significantly up-regulated the transcript levels of P5CS2, P5CR, OAT, DAO4 and down-regulated the transcript levels of BADH2. Exogenous procyanidins had no substantial effects on plant height, stem diameter, fresh weight, and dry weight of aromatic rice plants. Conclusions In conclusion, our findings reported the increment of 2-AP content in aromatic rice under exogenous procyanidins. Our results indicated that the application of exogenous procyanidins enhanced 2-AP biosynthesis by improving proline biosynthesis and inhibiting GABA formation.

show abstract

Section: Determination Of Proline Gaba P5c Methylglyoxal and 1-pyrrol...mentioning

confidence: 99%

Foliar application of procyanidins enhanced the biosynthesis of 2-acetyl-1-pyrroline in aromatic rice (Oryza sativa L.)

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Our previous study also confirmed that NH 4 + -fed rice seedlings showed a significantly dosedependent increase in Pro in shoots, while the innate level of Pro in NO 3 − -fed rice seedlings is independent of the NO 3 − dose supplied. Additionally, we noticed that accumulation of Pro was observable in rice plants supplied with additional Glu and Arg, where the latter demonstrated much higher potential than the former during the synthesis of Pro in rice plants [12].…”

Section: Introductionmentioning

confidence: 86%

“…Seedlings of similar sizes were selected for the following treatments (Figure 1). (1) 'Cr(III)+(−NI)' treatments: Rice seedlings were pre-treated with the nutrition solution without KNO3/NH4Cl ('−NI'), but with 3 mM Arg (+NArg) or 10 mM Glu (+NGlu) for 12 h [12], and subsequently exposed to Cr(III) solution at 0, 12.0, 24.0 and 40.0 mg Cr/L for 2 days (Figure 1).…”

Section: Rice Seedlings and Cr Treatmentmentioning

confidence: 99%

“…Rice is one of the world's most produced crops and a major energy source in the world. Research on the synthesis of Pro in rice plants from both inorganic (NO 3 − and NH 4 + ) and organic (Arg and Glu) N sources showed a completely different increment pattern [12]. However, no information is available to investigate the endogenous Pro in rice plants fertilized with different nitrogenous chemicals under Cr(III) stress.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mathematical Estimation of Endogenous Proline as a Bioindicator to Regulate the Stress of Trivalent Chromium on Rice Plants Grown in Different Nitrogenous Conditions

Li,

Feng,

Tian

et al. 2023

Toxics

Self Cite

View full text Add to dashboard Cite

The accumulation of proline impacts the defense mechanisms of plants against the harmful effects of adverse environmental conditions; however, its concentration in plants is associated with the metabolism of N. Therefore, the effects of exogenous organic [glutamate (Glu)/arginine (Arg)] and inorganic [nitrate (NO3−)/ammonium (NH4+)] N on the accumulation of proline (Pro) in rice plants under trivalent chromium [Cr(III)] stress were studied through using the mass balance matrix model (MBMM). Application of ‘NH4+’ showed the largest contribution to the Pro content in rice shoots under different concentrations of Cr(III), followed by ‘NO3−’, ‘Arg’, and ‘Glu’ applications. On the other hand, ‘Arg’ application displayed the largest contribution to the Pro content in roots under Cr(III) stress, followed by ‘NH4+’, ‘Glu’, and ‘NO3−’ applications. The combined application of ‘NH4++Arg’ showed the greatest contribution to the Pro content in both roots and shoots of Cr(III)-treated rice seedlings, while the application of ‘NO3−+Glu’ showed the least contribution to the Pro content in rice seedlings. The current study indicated that the endogenous level of Pro in rice seedlings is quite sensitive to Cr(III) stress under different N sources, and the mathematical modeling showed a reliable result while estimating the relationship between Pro content and N source application.

show abstract

“…However, few comparative studies have analyzed the effects of nitrogen fertilizers and plant growth regulators on salt stress in rice seedlings. The positive effects of 5-ALA, DTA-6, and nitrogen fertilizers on plant adversity have been explored in other crops [ 25 , 26 ]. However, the regulatory mechanisms and beneficial effects of 5-ALA, DTA-6, and nitrogen fertilizers on salt stress in rice are still unclear.…”

Section: Introductionmentioning

confidence: 99%

5-ALA, DTA-6, and Nitrogen Mitigate NaCl Stress by Promoting Photosynthesis and Carbon Metabolism in Rice Seedlings

Wang,

Tan,

et al. 2024

Metabolites

View full text Add to dashboard Cite

A large number of dead seedlings can occur in saline soils, which seriously affects the large-scale cultivation of rice. This study investigated the effects of plant growth regulators (PGRs) and nitrogen application on seedling growth and salt tolerance (Oryza sativa L.), which is of great significance for agricultural production practices. A conventional rice variety, “Huang Huazhan”, was selected for this study. Non-salt stress treatments included 0% NaCl (CK treatment), CK + 0.05 g N/pot (N treatment), CK + 40 mg·L−1 5-aminolevulinic acid (5-ALA) (A treatment), and CK + 30 mg·L−1 diethylaminoethyl acetate (DTA-6) (D treatment). Salt stress treatments included 0.3% NaCl (S treatment), N + 0.3% NaCl (NS treatment), A + 0.3% NaCl (AS treatment), and D + 0.3% NaCl (DS treatment). When 3 leaves and 1 heart emerged from the soil, plants were sprayed with DTA-6 and 5-ALA, followed by the application of 0.3% NaCl (w/w) to the soil after 24 h. Seedling morphology and photosynthetic indices, as well as carbohydrate metabolism and key enzyme activities, were determined for each treatment. Our results showed that N, A, and D treatments promoted seedling growth, photosynthesis, carbohydrate levels, and the activities of key enzymes involved in carbon metabolism when compared to the CK treatment. The A treatment had the most significant effect, with increases in aboveground dry weight and net photosynthetic rates (Pn) ranging from 17.74% to 41.02% and 3.61% to 32.60%, respectively. Stomatal limiting values (Ls) significantly decreased from 19.17% to 43.02%. Salt stress significantly inhibited seedling growth. NS, AS, and DS treatments alleviated the morphological and physiological damage of salt stress on seedlings when compared to the S treatment. The AS treatment was the most effective in improving seedling morphology, promoting photosynthesis, increasing carbohydrate levels, and key enzyme activities. After AS treatment, increases in aboveground dry weight, net photosynthetic rate, soluble sugar content, total sucrose synthase, and amylase activities were 17.50% to 50.79%, 11.39% to 98.10%, 20.20% to 80.85%, 21.21% to 33.53%, and 22.17% to 34.19%, respectively, when compared to the S treatment. In summary, foliar sprays of 5-ALA, DTA-6, and additional nitrogen fertilizer enhanced rice seedling growth, increased photosynthesis, lowered Ls values, and improved seedling salt tolerance. Spraying two regulators, 5-ALA and DTA-6, quantitatively increased the effect of nitrogen fertilizer, with comparable effects on NaCl stress regulation. This study provides the basis for efficient agricultural production.

show abstract

Elucidating comportment of the glutamate and ornithine pathway on proline accumulation in rice under different nitrogenous nutrition

Cited by 7 publications

References 31 publications

Foliar application of procyanidins enhanced the biosynthesis of 2-acetyl-1-pyrroline in aromatic rice (Oryza sativa L.)

Foliar application of procyanidins enhanced the biosynthesis of 2-acetyl-1-pyrroline in aromatic rice (Oryza sativa L.)

Mathematical Estimation of Endogenous Proline as a Bioindicator to Regulate the Stress of Trivalent Chromium on Rice Plants Grown in Different Nitrogenous Conditions

5-ALA, DTA-6, and Nitrogen Mitigate NaCl Stress by Promoting Photosynthesis and Carbon Metabolism in Rice Seedlings

Contact Info

Product

Resources

About