Elongation factor P: mechanism of action and opportunities for drug design

Mudryi, Vitalii

doi:10.53846/goediss-10005

Cited by 1 publication

(2 citation statements)

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“…We found the unique recognition elements of an EF-P/EfpL dependent arrest motif to be the P-site tRNA Pro and the E-site codon, in agreement with past studies 9,10,38 . We therefore wondered whether XP -regardless of being part of a stalling motif or not -promotes binding of EF-P and similarly EfpL to the ribosome.…”

Section: Ef-p and Efpl Can Induce Translational Pausessupporting

confidence: 92%

“…Accordingly, in the ideal case, the EF-P retention time on the ribosome could be modulated according to the motif's arrest strength. Indeed, the dissociation rate constant of EF-P from the ribosome differs depending on the E-site codon 38 . Our data support the hypothesis that amino acids encoded by a codon beginning with a guanosine induce a particularly strong translational arrest in XP(P) motifs (Fig.…”

Section: Discussionmentioning

confidence: 99%

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EF-P and its paralog EfpL (YeiP) differentially control translation of proline-containing sequences

Sieber,

Parr,

von Ehr

et al. 2024

Preprint

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Polyproline sequences (XPPX) stall ribosomes, thus being deleterious for all living organisms. In bacteria, translation elongation factor P (EF-P) plays a crucial role in overcoming such arrests. 12% of eubacteria possess an EF-P paralog - YeiP (EfpL) of unknown function. Here, we functionally and structurally characterize EfpL from Escherichia coli and demonstrate its yet unrecognized role in the translational stress response. Through ribosome profiling, we analyzed the EfpL arrest motif spectrum and discovered additional stalls beyond the canonical XPPX motifs at single-proline sequences (XPX), that both EF-P and EfpL can resolve. Notably, the two factors can also induce pauses. We further report that, contrary to the housekeeping EF-P, EfpL can sense the metabolic state of the cell, via lysine acylation. Together, our work uncovers a new player in ribosome rescue at proline-containing sequences, and provides evidence that co-occurrence of EF-P and EfpL is an evolutionary driver for higher bacterial growth rates.

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Section: Ef-p and Efpl Can Induce Translational Pausessupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%