Elongation Factor P Interactions with the Ribosome Are Independent of Pausing

Tollerson, Rodney; Witzky, Anne; Ibba, Michael

doi:10.1128/mbio.01056-17

Cited by 4 publications

(2 citation statements)

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“…Elongation factor P-like protein YeiP is a subtype of the elongation factor P (EF-P), which can only be produced by Enterobacteriales (Adeolu et al, 2016;Mohapatra et al, 2017). After lysis of Enterobacteriales, the DNA sequence might transfer into the human genome of B cells to cause abnormal translation of the protein (Tollerson et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiota in Untreated Diffuse Large B Cell Lymphoma Patients

Wang

Zhang

et al. 2021

Front. Microbiol.

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Intestinal microecology plays an important role in the development and progression of hematological malignancies. However, characteristics of gut microbiota in diffuse large B cell lymphoma (DLBCL) have not been reported. The microbiota composition of fecal samples from 25 untreated DLBCL patients and 26 healthy volunteers was examined by 16S rRNA gene sequencing. On α-diversity analysis, there was no significant difference in species diversity and abundance between the two groups. However, a significant difference was observed on β-diversity analysis. The intestinal microbiota in patients with DLBCL showed a continuous evolutionary relationship, which progressed from phylum, proteobacteria, to genus, Escherichia-Shigella. Their abundance was significantly higher than that of the control group. At the genus level, Allisonella, lachnospira, and Roseburia were more abundant in patients with DLBCL than in the control group. Functional prediction by PICRUSt indicated that thiamine metabolism and phenylalanine, tyrosine, and tryptophan biosynthesis were significantly lower in the DLBCL group than in the control group. In conclusion, our results clearly demonstrate that the gut microbiota was changed significantly in DLBCL. The study highlights fundamental differences in the microbial diversity and composition of patients with DLBCL and paves the way for future prospective studies and microbiome-directed interventional trials to improve patient outcomes.

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Section: Discussionmentioning

confidence: 99%

Gut Microbiota in Untreated Diffuse Large B Cell Lymphoma Patients

Wang

Zhang

et al. 2021

Front. Microbiol.

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“…EF-P is a ubiquitous prokaryotic homologue of eukaryotic eIF5A, a protein that promotes translation of proline-rich motifs (5). The association of EF-P with the ribosome is transient and dynamic (6). EF-P can diminish ribosomal stalling at proline-rich regions of polypeptides by stimulating peptide bond formation in the ribosome (7)(8)(9).…”

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confidence: 99%

Extragenic Suppression of Elongation Factor P Gene Mutant Phenotypes in Erwinia amylovora

et al. 2019

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Elongation factor P (EF-P) facilitates the translation of certain peptide motifs, including those with multiple proline residues. EF-P must be posttranslationally modified for full functionality; in enterobacteria, this is accomplished by two enzymes, namely, EpmA and EpmB, which catalyze the β-lysylation of EF-P at a conserved lysine position. Mutations to efp or its modifying enzymes produce pleiotropic phenotypes, including decreases in virulence, swimming motility, and extracellular polysaccharide production, as well as proteomic perturbations. Here, we generated targeted deletion mutants of the efp, epmA, and epmB genes in the Gram-negative bacterium Erwinia amylovora, which causes fire blight, an economically important disease of apples and pears. As expected, the Δefp, ΔepmA, and ΔepmB mutants were all defective in virulence on apples, and all three mutants were complemented in trans with plasmids bearing wild-type copies of the corresponding genes. By analyzing spontaneous suppressor mutants, we found that mutations in the hrpA3 gene partially or completely suppressed the colony size, extracellular polysaccharide production, and virulence phenotypes in apple fruits and apple tree shoots but not the swimming motility phenotypes of the Δefp, ΔepmA, and ΔepmB mutants. The deletion of hrpA3 alone did not produce any alterations in any characteristics measured, indicating that the HrpA3 protein is not essential for any of the processes examined. The hrpA3 gene encodes a putative DEAH-box ATP-dependent RNA helicase. These results suggest that the loss of the HrpA3 protein at least partially compensates for the lack of the EF-P protein or β-lysylated EF-P. IMPORTANCE Fire blight disease has relatively few management options, with antibiotic application at bloom time being chief among them. As modification to elongation factor P (EF-P) is vital to virulence in several species, both EF-P and its modifying enzymes make attractive targets for novel antibiotics. However, it will be useful to understand how bacteria might overcome the hindrance of EF-P function so that we may be better prepared to anticipate bacterial adaptation to such antibiotics. The present study indicates that the mutation of hrpA3 could provide a partial offset for the loss of EF-P activity. In addition, little is known about EF-P functional interactions or the HrpA3 predicted RNA helicase, and our genetic approach allowed us to discern a novel gene associated with EF-P function.

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