Elongated virus particles in plasmodesmata

Weintraub, M.; Ragetli, H. W. J.; Leung, Esther

doi:10.1016/s0022-5320(76)90010-1

Cited by 36 publications

(13 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is consistent with a previous study of PVX (Allison and Shalla, 1974) and also with reports of other filamentous viruses localizing to the plasmodesmal pore (Esau et al, 1967;Weintraub et al, 1976;discussed in Lucas et al, 1990). Intercellular movement of the filamentous PVX virion implies a mechanism distinct from the CP-independent transport process used by TMV.…”

Section: Discussionsupporting

confidence: 93%

Cell-to-Cell and Phloem-Mediated Transport of Potato Virus X: The Role of Virions

Cruz¹,

Roberts²,

Prior³

et al. 1998

Plant Cell

183

View full text Add to dashboard Cite

Movement-deficient potato virus X (PVX) mutants tagged with the green fluorescent protein were used to investigate the role of the coat protein (CP) and triple gene block (TGB) proteins in virus movement. Mutants lacking either a functional CP or TGB were restricted to single epidermal cells. Microinjection of dextran probes into cells infected with the mutants showed that an increase in the plasmodesmal size exclusion limit was dependent on one or more of the TGB proteins and was independent of CP. Fluorescently labeled CP that was injected into epidermal cells was confined to the injected cells, showing that the CP lacks an intrinsic transport function. In additional experiments, transgenic plants expressing the PVX CP were used as rootstocks and grafted with nontransformed scions. Inoculation of the PVX CP mutants to the transgenic rootstocks resulted in cell-to-cell and systemic movement within the transgenic tissue. Translocation of the CP mutants into sink leaves of the nontransgenic scions was also observed, but infection was restricted to cells close to major veins. These results indicate that the PVX CP is transported through the phloem, unloads into the vascular tissue, and subsequently is transported between cells during the course of infection. Evidence is presented that PVX uses a novel strategy for cell-to-cell movement involving the transport of filamentous virions through plasmodesmata.

show abstract

Section: Discussionsupporting

confidence: 93%

Cell-to-Cell and Phloem-Mediated Transport of Potato Virus X: The Role of Virions

Cruz¹,

Roberts²,

Prior³

et al. 1998

Plant Cell

183

View full text Add to dashboard Cite

show abstract

“…If indeed these structures found opposite the plasmodesmata are involved in viral transport, then such transport probably occurs in the form of viral RNA since viral capsid protein was found neither in the plasmodesmata nor in the CI protein strands associated with the plasmalemma. These results are in contrast to those of Weintraub et al (1976) who found intact virions of another potyvirus, namely potato virus Y, in plasmodesmata. Since the CI protein contains NTP-binding and helicase motifs (Domier et al, 1987;Lain et al, 1989a, b) it is reasonable to expect that the CI protein found attached to the plasmalemma at the early stages of infection represents viral RNA replication complexes.…”

Section: The CL Proteincontrasting

confidence: 99%

A temporal study of the expression of the capsid, cytoplasmic inclusion and nuclear inclusion proteins of tobacco etch potyvirus in infected plants

Baunoch

Das

Browning

et al. 1991

Journal of General Virology

View full text Add to dashboard Cite

Young leaves of tobacco, systemically infected by tobacco etch potyvirus (TEV), were examined for the presence and distribution of four virus encoded proteins [capsid, cytoplasmic inclusion (CI) and two nuclear inclusion (NI) proteins] at various time periods after inoculation of expanded leaves of the plants. The analyses were carried out by ELISA and by immunogold electron microscopy of thin sections of the leaves. All four proteins were detected simultaneously in the systemic leaves for the first time on the fifth day after inoculation of the expanded leaves. All four proteins increased in concentration until the seventh day and then showed no further increase with the exception of the capsid protein which continued to accumulate. The CI protein was first detected in association with the plasmalemma/cell wall and was subsequently found mostly in the form of pinwheels in the cytoplasm. The two NI proteins were found at all times after infection within the nucleus, although small concentrations were detected in the cytoplasm. These experiments suggest that both the NIa and NIb proteins are transported into the nucleus immediately after synthesis. At the earliest time periods after infection, high concentrations of these proteins (NIa and NIb) were found in their noninclusion form in the nucleolus. At 14 days after infection, both proteins were found only as inclusions in the nucleus. The capsid protein was found at all stages of infection only in the cytoplasm.

show abstract

“…Based on the striking morphological and dimensional similarity between the TMV MP-associated filaments and those putative TMV particles, we interpret those putative TMV particles present in the plasmodesmata described by Weintraub et al (1976) to be MP-associated filaments. Consequently, there is no solid evidente to suggest intercellular movement of TMV particles.…”

Section: Virus Movement and Macromolecular Trafficking Through Plasmomentioning

confidence: 93%

“…The presence of "TMV particles" in mesophyll plasmodesmata in TMV-infected tobacco, as reported by Weintraub et al (1976), has been used as evidence to argue that TMV moves from cell to cell as an intact virion. Based on the striking morphological and dimensional similarity between the TMV MP-associated filaments and those putative TMV particles, we interpret those putative TMV particles present in the plasmodesmata described by Weintraub et al (1976) to be MP-associated filaments.…”

Section: Virus Movement and Macromolecular Trafficking Through Plasmomentioning

confidence: 94%

Secondary plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in transgenic tobacco plants.

et al. 1992

View full text Add to dashboard Cite

Expresslon of the tobacco mosalc virus 30-kD movement protein (TMV MP) gene in tobacco plants increases the plasmodesmatal size exclusion limlt (SEL) 10-fold between mesophyll cells in mature leaves. In the present study, we examined the structure of plasmodesmata as a functlon of leaf development. In young leaves of 30-kD TMV MP transgenic (line 274) and vector control ( l h e 306) plants, almost all plasmodesmata were primary in nature. In both plant lines, secondary plasmodesmata were formed, in a basipetal pattern, as the leaves underwent expansion growth. Ultrastructural and immunolabellng studies demonstrated that ln line 274 the TMV MP accumulated predominantly in secondary plasmodesmata of nonvascular tissues and was associated with a filamentous material. A developmental progression was detected in terms of the presence of TMV MP; all secondary plasmodesmata in the tip of the fourth leaf contained TMV MP in association with the filamentous material. Dye-coupling experiments demonstrated that the TMV MP-induced increase in plasmodesmatal SEL could be routinely detected in the tip of the fourth leaf, but was restricted to mesophyll and bundle sheath cells. These findings are dlscussed with respect to the structure and function of plasmodesmata, particularly those aspects related to virus movement.

show abstract

Elongated virus particles in plasmodesmata

Cited by 36 publications

References 16 publications

Cell-to-Cell and Phloem-Mediated Transport of Potato Virus X: The Role of Virions

Cell-to-Cell and Phloem-Mediated Transport of Potato Virus X: The Role of Virions

A temporal study of the expression of the capsid, cytoplasmic inclusion and nuclear inclusion proteins of tobacco etch potyvirus in infected plants

Secondary plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in transgenic tobacco plants.

Contact Info

Product

Resources

About