Ellipsometry Studies of Lipoprotein Adsorption

Malmsten, Martin; Siegel, G.; Wood, W. G.

doi:10.1006/jcis.1999.6689

Cited by 36 publications

(23 citation statements)

References 60 publications

(74 reference statements)

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“…Moreover, figure 2 demonstrates that beyond nanoplaque formation and deposition (A) also its dimensional build-up (B) is a Ca 2+ -driven process. Adsorbed layer thickness which is related to the molecular size of the ternary aggregational complexes markedly increased upon Ca1 (2.52 mmol/l) and remained practically constant upon Ca2 additions up to 10.08 mmol/l, in complete accordance with former experiments in the absence of proteoglycan coating of the silica surface [15] and with light scattering experiments [24], where the adsorbed amount and particle size, respectively, increased significantly with Ca2 incubation. The augmentation in adsorbed layer thickness (nanoplaque size) with increasing Ca 2+ concentration took a distinctly reduced course after 3 months of therapy as compared to the control curve.…”

Section: Resultssupporting

confidence: 73%

“…50]. In support of this result it may be stated that under high Ca 2+ conditions nanoplaque formation per se is much more pronounced using the atherogenic apoB100-fraction existing at a higher concentration compared to the concentration of Lp(a) or oxLDL [24]. The Ca 2+ concentration range applied in our experiments is justified, because counterion condensation of calcium up to a concentration of 25 mmol/l can appear in the immediate vicinity of the PG receptors with their highly negative charge density [51,52].…”

Section: Discussionsupporting

confidence: 71%

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Reduction of Arteriosclerotic Nanoplaque Formation and Size by n-3 Fatty Acids in Patients after Valvular Defect Operation

Rodríguez¹,

Winkler²,

Pietzsch³

et al. 2009

Complement Med Res

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Background/Methods: Coating a silica surface with the isolated lipoprotein receptor heparan sulfate proteoglycan (HS-PG) from arterial endothelium and vascular matrices, we could observe the very earliest stages of arteriosclerotic plaque development by ellipsometric techniques in vitro (patent EP 0 946 876). This so-called nanoplaque formation is represented by the ternary aggregational complex of the HS-PG receptor, lipoprotein particles and calcium ions. The model was validated in several clinical studies on statins in cardiovascular high-risk patients applying their native blood lipoprotein fractions. Results: In 7 patients who had undergone a valvular defect operation, the reduction of arteriosclerotic nanoplaque formation in normal Krebs solution amounted to 6.1 ± 2.3% (p < 0.0156) and of nanoplaque size to 37.5 ± 13.2% (p < 0.0312), respectively, after a 3-month therapy with n-3 fatty acids (3 ··3 g daily, Ameu® 500 mg). Additionally, the quotient oxLDL/LDL was lowered by 6.8 ± 2.1% (p < 0.0166), the MDA concentration remained unchanged and the lipoprotein(a) concentration decreased by 15.8 ± 5.6% (p < 0.0469) in the patients’ blood. The concentration of the nanoplaque promoting particles VLDL and total triglycerides was diminished by 34.1 ± 11.6% (p < 0.0469) and 26.7 ± 10.8% (p < 0.0156), respectively. Furthermore, the ratio of the strongly atherogenic small dense to the total LDL cholesterol (LDL5+LDL6)/LDLtot decreased by 9.9 ± 3.0% (p < 0.0174). Conclusions: A combinatorial regression analysis revealed a basis for a mechanistic explanation of nanoplaque reduction under n-3 fatty acid treatment. This effect was possibly due to the beneficial changes in lipid concentrations and an attenuation of the risk factors oxLDL/LDL and (LDL5+LDL6)/LDLtot.

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Section: Resultssupporting

confidence: 73%

Section: Discussionsupporting

confidence: 71%

Reduction of Arteriosclerotic Nanoplaque Formation and Size by n-3 Fatty Acids in Patients after Valvular Defect Operation

Rodríguez¹,

Winkler²,

Pietzsch³

et al. 2009

Complement Med Res

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“…Recently, it was found that transthyretin and apolipoprotein A-IV can form a multiprotein complex in human plasma [70]. The adsorption of all these proteins onto biomaterials may occur for free single proteins (Apo A-I binds to a variety of biomaterials [60]), transfer of proteins from HDL or direct adsorption of HDLs, which interact strongly with hydrophobic alkyl methacrylate polymers, polyethylene, and silica during plasma contact [62][63][64], although the role of apolipoproteins in biocompatibility was never explained. We suggest that HDLs may increase biocompatibility through specific protein-receptor interactions.…”

Section: Discussionmentioning

confidence: 99%

Two‐step elution of human serum proteins from different glass‐modified bioactive surfaces: A comparative proteomic analysis of adsorption patterns

et al. 2004

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Plasma protein adsorption patterns on surfaces may give vital information to evaluate biocompatibility of biomaterials designed for direct blood-contacting applications or tissue integration. Adsorption of human serum proteins on four different types of biomaterials (glass, aminosilanized glass, hyaluronan and sulfated hyaluronan) was analyzed by two-dimensional electrophoresis. Desorption of proteins from the surfaces was first classically achieved by sodium dodecyl sulfate (SDS) elution. We introduced a second elution step (by use of isoelectric focusing (IEF) sample buffer consisting of urea, 3-[(3-cholamidopropyl)dimethylammonio]-1-propansulfonate, and dithioerythritol) which allows more stringent elution conditions and is a tool to evaluate the protein adsorption strength to biomaterials. Moreover, the two-step elution may discriminate between irreversible and reversible adsorption of plasma proteins for biomaterials, thus helping to elucidate the structure of protein multilayers which form a complex system at the surfaces. The IEF sample buffer proved not to alter the biomaterial structure and integrity. Hydrophobic bonds resulted to be the main strength driving protein adsorption onto our biomaterials. Apolipoproteins were the most important proteins interacting with the surfaces suggesting that high-density lipoprotein (HDL) particles could play a role in biocompatibility due to their beneficial effects on endothelial cells.

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“…Considering this, we previously investigated the interfacial behavior of a number of lipoproteins, notably at surfaces modified through adsorption of proteoheparan sulfate in an attempt to mimic the surface exposed to the lipoproteins in the blood vessel (2). Although this certainly constitutes a simplification compared to the in vivo situation, a qualitative agreement was obtained between ellipsometry studies with the model substrates, on one hand, and the role of the lipoproteins in the clinical situation, on the other.…”

Section: Introductionmentioning

confidence: 87%

A Model Substrate for Ellipsometry Studies of Lipoprotein Deposition at the Endothelium

Malmsten

Siegel

Becker

2001

Journal of Colloid and Interface Science

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Ellipsometry Studies of Lipoprotein Adsorption

Cited by 36 publications

References 60 publications

Reduction of Arteriosclerotic Nanoplaque Formation and Size by n-3 Fatty Acids in Patients after Valvular Defect Operation

Reduction of Arteriosclerotic Nanoplaque Formation and Size by n-3 Fatty Acids in Patients after Valvular Defect Operation

Two‐step elution of human serum proteins from different glass‐modified bioactive surfaces: A comparative proteomic analysis of adsorption patterns

A Model Substrate for Ellipsometry Studies of Lipoprotein Deposition at the Endothelium

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