Ellagic acid: Pharmacological activities and molecular mechanisms involved in liver protection

García-Niño, Wylly Ramsés; Zazueta, Cecilia

doi:10.1016/j.phrs.2015.04.008

Cited by 206 publications

(132 citation statements)

References 373 publications

(94 reference statements)

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“…Another biomarker, ellagic acid has gained much importance in the recent years due to its multiple pharmacological activities and molecular targets. It has shown several antibacterial, anticarcinogenic and antiatherogenic activities (Garcia-Nino and Zazueta, 2015). Several studies have enumerated the role of ellagic acid in effective lowering of plasma lipids, reduced oxidative stress, inhibition of apoptosis, scavengers of oxygen and hydroxyl radicals as well as inhibition of lipid peroxidation both in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Potential of Terminalia chebula by Hydrogen Peroxide Induced Oxidative Stress in THP-1 Cell Line

Minakshi¹,

Vasanth²,

Tanupriya³

et al. 2014

International J. of Botany

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A B S T R A C T Diseases caused due to 'oxidative stress' initiated by Reactive Oxygen Species (ROS) is growing at an alarming rate. Several medicinal herbs explored till date were reported to possess numerous pharmacological properties used for combating different diseases. The present study was undertaken to investigate the inhibitory potential of Terminalia chebula by H 2 O 2 induced oxidative stress in THP-1 cell line and also the presence of the marker compounds in T. chebula was confirmed by High Performance Liquid Chromatography (HPLC). Intracellular quantification of ROS was done using a flow cytometer. The percentage of gallic acid and ellagic acid in the extract was performed by HPLC were found to be 4.84% w/w and 2.56% w/w, respectively. Pretreatment of cells with T. chebula extract significantly reduced the intracellular ROS production in a dose-dependent manner. The present study illustrates the effective role of T. chebula in reducing the ROS production which is a causative factor for several disorders. The results describe the possibility and potentiality of natural sources to inhibit the pathogenesis of different diseases caused due to oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Potential of Terminalia chebula by Hydrogen Peroxide Induced Oxidative Stress in THP-1 Cell Line

Minakshi¹,

Vasanth²,

Tanupriya³

et al. 2014

International J. of Botany

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“…Through the action of human colonic microflora, EA is partially converted into metabolites, including hydroxy-6H-benzopyran-6-one derivatives, primarily urolithin A (UA) [12]. Both, EA and urolithins enter the circulation.…”

Section: Introductionmentioning

confidence: 99%

Negative Effect of Ellagic Acid on Cytosolic pH Regulation and Glycolytic Flux in Human Endometrial Cancer Cells

Abdelazeem

Singh

Läng

et al. 2017

Cell Physiol Biochem

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Background/Aims: Key properties of tumor cells include enhanced glycolytic flux with excessive consumption of glucose and formation of lactate. As glycolysis is highly sensitive to cytosolic pH, maintenance of glycolysis requires export of H⁺ ions, which is in part accomplished by Na⁺/H⁺ exchangers, such as NHE1. The carrier is sensitive to oxidative stress. Growth of tumor cells could be suppressed by the polyphenol Ellagic acid, which is found in various fruits and vegetables. An effect of Ellagic acid on transport processes has, however, never been reported. The present study thus elucidated an effect of Ellagic acid on cytosolic pH (pHi), NHE1 transcript levels, NHE1 protein abundance, Na⁺/H⁺ exchanger activity, and lactate release. Methods: Experiments were performed in Ishikawa cells without or with prior Ellagic acid (20 µM) treatment. NHE1 transcript levels were determined by qRT-PCR, NHE1 protein abundance by Western blotting, pH_i utilizing (2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein [BCECF] fluorescence, Na⁺/H⁺ exchanger activity from Na⁺ dependent realkalinization after an ammonium pulse, cell volume from forward scatter in flow cytometry, reactive oxygen species (ROS) from 2’,7’-dichlorodihydrofluorescein fluorescence, glucose uptake utilizing 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose, and lactate concentration in the supernatant utilizing a colorimetric (570 nm)/ fluorometric enzymatic assay. Results: A 48 hour treatment with Ellagic acid (20 µM) significantly decreased NHE1 transcript levels by 75%, NHE1 protein abundance by 95%, pHi from 7.24 ± 0.01 to 7.02 ± 0.01, Na⁺/H⁺ exchanger activity by 77%, forward scatter by 10%, ROS by 82%, glucose uptake by 58%, and lactate release by 15%. Conclusion: Ellagic acid (20µM) markedly down-regulates ROS formation and NHE1 expression leading to decreased Na⁺/H⁺ exchanger activity, pHi, glucose uptake and lactate release in endometrial cancer cells. Those effects presumably contribute to reprogramming and growth inhibition of tumor cells.

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“…It is also worth mentioning that ellagic acid has received special attention from researchers because of its pluripotent biological activity and the multiple molecular targets that it acts upon [102]. Based on this, an animal model of hepatocellular carcinoma demonstrated that the oral administration of ellagic acid (50 mg/ Kg/day), 7 days before and 14 days after tumour induction (N-nitrosodiethylamine and CCl 4 ), provided 23.6 % of inhibition of ARG activity when compared to the negative control group (healthy rats).…”

Section: Methodsmentioning

confidence: 99%

“…Indeed, the amount of ellagitannins in systemic circulation and tissues is virtually undetectable, whereas urolithins and their conjugates can be found in higher levels (µM). It has been reported that ellagitannin metabolites can be detected in the liver and kidneys [106], urolithins are enhanced in the prostate, intestinal tissue, and colon in mice, and urolithin A-glucuronide is the main metabolite found in the human prostate (> 2 ng/g tissue) as well as traces of urolithin B-glucuronide and ellagic acid-dimethyl ether [102,107].…”

Section: Methodsmentioning

confidence: 99%

Untitled

2018

Planta Med

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IntroductionSeveral diseases have been linked to the development of ED, such as atherosclerosis, hypercholesterolaemia, coronary disease, erectile dysfunction, asthma, renal failure, rheumatoid arthritis, periodontitis, psychiatric disorders, and cancer, as well as diseases with a high prevalence, such as diabetes (types 1 and 2) and SAH [1-9].The preservation of the endothelium is fundamental in maintaining the physiology of the vascular system (in the regulation of its tonus, the development of immune, structural, and proliferative functions, and interaction with other cellular types) and also in the prevention of the development/aggravation of diseases [10][11][12].

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Ellagic acid: Pharmacological activities and molecular mechanisms involved in liver protection

Cited by 206 publications

References 373 publications

Inhibitory Potential of Terminalia chebula by Hydrogen Peroxide Induced Oxidative Stress in THP-1 Cell Line

Inhibitory Potential of Terminalia chebula by Hydrogen Peroxide Induced Oxidative Stress in THP-1 Cell Line

Negative Effect of Ellagic Acid on Cytosolic pH Regulation and Glycolytic Flux in Human Endometrial Cancer Cells

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