Ellagic acid inhibits oxidized LDL-mediated LOX-1 expression, ROS generation, and inflammation in human endothelial cells

Lee, Wen Jane; Ou, Hsiu Chung; Hsu, Wen Cheng; Chou, Min-Min; Tseng, Jen Yu; Hsu, Shih-Kuang; Tsai, Kun Ling; Sheu, Wayne Huey Herng

doi:10.1016/j.jvs.2010.04.085

Cited by 143 publications

(113 citation statements)

References 39 publications

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“…Previous studies have revealed apoptosis in VECs with elevated intracellular ROS levels [39,40]. Intracellular ROS levels are regulated by the balance between ROS generation and antioxidant enzymes [41]. Our results showed that elevated ROS levels induced by oxLDL were decreased significantly with PT pretreatment.…”

Section: Discussionsupporting

confidence: 60%

Pterostilbene protects vascular endothelial cells against oxidized low-density lipoprotein-induced apoptosis in vitro and in vivo

et al. 2011

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Vascular endothelial cell (VEC) apoptosis is the main event occurring during the development of atherosclerosis. Pterostilbene (PT), a natural dimethylated analog of resveratrol, has been the subject of intense research in cancer and inflammation. However, the protective effects of PT against oxidized low-density lipoprotein (oxLDL)-induced apoptosis in VECs have not been clarified. We investigated the anti-apoptotic effects of PT in vitro and in vivo in mice. PT at 0.1-5 μM possessed antioxidant properties comparable to that of trolox in a cell-free system. Exposure of human umbilical vein VECs (HUVECs) to oxLDL (200 μg/ml) induced cell shrinkage, chromatin condensation, nuclear fragmentation, and cell apoptosis, but PT protected against such injuries. In addition, PT injection strongly decreased the number of TUNEL-positive cells in the endothelium of atherosclerotic plaque from apoE(-/-) mice. OxLDL increased reactive oxygen species (ROS) levels, NF-κB activation, p53 accumulation, apoptotic protein levels and caspases-9 and -3 activities and decreased mitochondrial membrane potential (MMP) and cytochrome c release in HUVECs. These alterations were attenuated by pretreatment with PT. PT inhibited the expression of lectin-like oxLDL receptor-1 (LOX-1) expression in vitro and in vivo. Cotreatment with PT and siRNA of LOX-1 synergistically reduced oxLDL-induced apoptosis in HUVECs. Overexpression of LOX-1 attenuated the protection by PT and suppressed the effects of PT on oxLDL-induced oxidative stress. PT may protect HUVECs against oxLDL-induced apoptosis by downregulating LOX-1-mediated activation through a pathway involving oxidative stress, p53, mitochondria, cytochrome c and caspase protease. PT might be a potential natural anti-apoptotic agent for the treatment of atherosclerosis.

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Section: Discussionsupporting

confidence: 60%

Pterostilbene protects vascular endothelial cells against oxidized low-density lipoprotein-induced apoptosis in vitro and in vivo

et al. 2011

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“…Several studies have enumerated the role of ellagic acid in effective lowering of plasma lipids, reduced oxidative stress, inhibition of apoptosis, scavengers of oxygen and hydroxyl radicals as well as inhibition of lipid peroxidation both in vitro and in vivo. It has been known to inhibit the cytokine induced ROS generation and expression of adhesion molecules as well as prevention of oxidized low Density Lipoprotein (LDL) induced apoptosis in the endothelial cells (Lee et al, 2010). The role of T. chebula in inhibiting ROS production may be attributed due to the symbiotic effect of these two important marker compounds present in the extract.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Potential of Terminalia chebula by Hydrogen Peroxide Induced Oxidative Stress in THP-1 Cell Line

Minakshi¹,

Vasanth²,

Tanupriya³

et al. 2014

International J. of Botany

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A B S T R A C T Diseases caused due to 'oxidative stress' initiated by Reactive Oxygen Species (ROS) is growing at an alarming rate. Several medicinal herbs explored till date were reported to possess numerous pharmacological properties used for combating different diseases. The present study was undertaken to investigate the inhibitory potential of Terminalia chebula by H 2 O 2 induced oxidative stress in THP-1 cell line and also the presence of the marker compounds in T. chebula was confirmed by High Performance Liquid Chromatography (HPLC). Intracellular quantification of ROS was done using a flow cytometer. The percentage of gallic acid and ellagic acid in the extract was performed by HPLC were found to be 4.84% w/w and 2.56% w/w, respectively. Pretreatment of cells with T. chebula extract significantly reduced the intracellular ROS production in a dose-dependent manner. The present study illustrates the effective role of T. chebula in reducing the ROS production which is a causative factor for several disorders. The results describe the possibility and potentiality of natural sources to inhibit the pathogenesis of different diseases caused due to oxidative stress.

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“…This in turn reduced local expression of cytokines like TNF-α and consequently may attenuate the potentiation of the intramyocardial inflammatory reaction. Hyperglycaemia (Li et al 2010a;Piga et al 2007), oxidised LDL (Lee et al 2010) (Hodgkinson et al 2008;Peterson et al 2007), and increased angiotensin II (Alvarez et al 2004;Rius et al 2010) under diabetes mellitus upregulate the expression of VCAM-1/ICAM-1. Since HDL did not affect blood glucose levels, the reduction in adhesion molecule expression was not an indirect consequence of an HDL-mediated antidiabetic effect.…”

Section: Human Apo A-i Gene Transfer Reduces Diabetes-induced Cardiacmentioning

confidence: 99%

Therapeutic Potential of HDL in Cardioprotection and Tissue Repair

Linthout

Frias²,

Singh

et al. 2014

High Density Lipoproteins

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Ellagic acid inhibits oxidized LDL-mediated LOX-1 expression, ROS generation, and inflammation in human endothelial cells

Cited by 143 publications

References 39 publications

Pterostilbene protects vascular endothelial cells against oxidized low-density lipoprotein-induced apoptosis in vitro and in vivo

Pterostilbene protects vascular endothelial cells against oxidized low-density lipoprotein-induced apoptosis in vitro and in vivo

Inhibitory Potential of Terminalia chebula by Hydrogen Peroxide Induced Oxidative Stress in THP-1 Cell Line

Therapeutic Potential of HDL in Cardioprotection and Tissue Repair

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