Ellagic acid induces apoptosis through inhibition of nuclear factor kB in pancreatic cancer cells

Edderkaoui, Mouad; Odinokova, Irina; Ohno, Izumi; Gukovsky, Ilya; Go, Vay Liang W; Pandol, Stephen J.; Gukovskaya, Anna S.

doi:10.3748/wjg.14.3672

Cited by 139 publications

(107 citation statements)

References 28 publications

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“…It has been shown to reduce the incidence of a variety of carcinogen-induced tumors 20 . The anticarcinogenic effect of EA was shown in several types of cancers [21][22][23][24][25] . In our study we observed, EA at 25-200µM strikingly reduced the cell viability of U251 cells, suggesting the anti-proliferative efficacy.…”

Section: Discussionmentioning

confidence: 99%

Ellagic acid inhibits proliferation and induces apoptosis in human glioblastoma cells

et al. 2016

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PURPOSE:To investigate the anticancer activity of ellagic acid (EA) in U251 human glioblastoma cells and its possible molecular mechanism. METHODS:The cells were treated with EA at various concentrations for different time periods. Cell viability and cell proliferation were detected by cell counting kit-8(CCK-8) assay and live/dead assay respectively. Cell apoptosis were measured with Annexin V-FITC/PI double staining method by flow cytometry and Mitochondrial membrane potential assay separately. Cell cycle was measured with PI staining method by flow cytometry. The expressions of Bcl-2, Survivin, XIAP, Caspase-3, Bax, JNK, p-JNK, ERK1/2, p-ERK1/2, p38, p-p38, DR4, DR5, CHOP and GRP78-related proteins were detected by western blot after EA treatment. RESULTS:Cell viability and proliferation of glioblastoma cells treated with EA were significantly lower than the control group. EA caused robust apoptosis of the glioblastoma cells compared to the control group. EA significantly decreased the proportion at G0/ G1 phases of cell cycling accompanied by increased populations at S phase in U251 cell lines. And the expressions of anti-apoptotic proteins were dramatically down-regulated. CONCLUSION:Ellagic acid potentially up-regulated DR4, DR5 and MAP kinases (JNK, ERK1/2 and p38). EA also caused significant increase in the expressions of CHOP and GRP78. Our findings suggest that EA would be beneficial for the treatment of glioblastoma.

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Section: Discussionmentioning

confidence: 99%

Ellagic acid inhibits proliferation and induces apoptosis in human glioblastoma cells

et al. 2016

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“…Clinical anti-cancer drugs are used for causing cell death by induction of apoptosis in cancer cells (38,39). DAS has been showed to promote immune responses in leukemic mice in vivo (29).…”

Section: Discussionmentioning

confidence: 99%

Diallyl sulfide induces cell cycle arrest and apoptosis in HeLa human cervical cancer cells through the p53, caspase- and mitochondria-dependent pathways

Chung

Liu

et al. 2011

Int J Oncol

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Abstract. Diallyl sulfide (DAS), one of the main active constituents of garlic, causes growth inhibition of cancer cells in vitro and promotes immune responses in vivo in experimental settings. However, its effects on the induction of cell cycle and apoptosis in human cervical cancer cells are still unclear. The aims of this study were to explore the anti-cancer effects of DAS in HeLa human cervical cancer cells and to investigate the underlying mechanisms in vitro. Cytotoxicity and apoptosis in HeLa human cervical cancer cells were examined by the morphological changes, viability assay, 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI) staining, comet assay, Western blotting and confocal microscopy examination. The results showed that DAS treatment for 24-72 h resulted in a marked decrease in cell viability time-and dose-dependently. Flow cytometric analysis showed that a 48-h treatment of 75 µM DAS induced G0/G1 cell cycle arrest and sub-G1 phase (apoptosis) in HeLa cells. Typical apoptotic nucleus alterations were observed by fluorescence microscopy in HeLa cells after exposure to DAS using DAPI staining. Cells treated with different concentrations of DAS also showed changes typical of apoptosis such as morphological changes, DNA damage and fragmentation, dysfunction of mitochondria, cytochrome c release and increased expression of pro-caspase-3 and -9. DAS also promoted the release of AIF and Endo G from mitochondria in HeLa cells. In conclusion, DAS induced G0/G1 cell cycle arrest and apoptosis in HeLa cells through caspase-and mitochondria and p53 pathways providing further understanding of the molecular mechanisms of DAS action in cervical cancer. This study, therefore, revealed that DAS significantly inhibits the growth and induces apoptosis of human cervical cancer HeLa cells in vitro.

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“…The presence of ellagic acid in human plasma is largely a consequence of their hydrolysis facilitated by physiological pH and activity of several colon microbiota [6]. Ellagic acid was found to reduce cancer cell growth by induction of apoptosis [21,22] and to exert various antiinflammatory effects [6]. The other biologically important phenolics are flavonol glycosides (including quercetin-3-glucuronide and kaempferol-3-glucuronide), flavan-3-ols (mainly (+)-catechin) and hydroxy acids (gallic, salicyl, caffeic, p-hydroxybenoic, ferulic, p-cumaric, cinnamic and vanillic acids) [5,12].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant properties of fruits of raspberry and blackberry grown in central Europe

et al. 2015

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Fruits of several, mainly Polish cultivars of floricane-and primocane-fruiting red raspberry (Rubus idaeus), black raspberry (Rubus occidentalis) and blackberry (Rubus fruticosus), grown in central Europe during two successive vegetation periods, were investigated. The content of phenolic compounds, including anthocyanins, as well as antioxidant properties of fruit extracts were analysed. A number of methods were employed: ferric ion reducing antioxidant power (FRAP), cupric ion reducing antioxidant capacity (CUPRAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity involving both colorimetric and EPR spectrometric measurements. From among all the tested fruits black raspberries had the largest antioxidant capacity as verified by all methods used in this study. These berries were also the most abundant in phenolic and anthocyanin compounds. Blackberries were characterised by larger antioxidant capacity than red raspberry fruits which were accompanied by higher content of total phenolics and anthocyanins. Berries of primocane-fruiting cultivars, often used for intensive agricultural production, generally did not differ in the total phenolic and anthocyanin content as well as in the antioxidant capacity as compared to the traditional, floricane-fruiting ones. The research contributes to deep characterisation of central European berry fruits which due to their high content and large diversity of health-beneficial compounds are classified as natural functional food.

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Ellagic acid induces apoptosis through inhibition of nuclear factor kB in pancreatic cancer cells

Cited by 139 publications

References 28 publications

Ellagic acid inhibits proliferation and induces apoptosis in human glioblastoma cells

Ellagic acid inhibits proliferation and induces apoptosis in human glioblastoma cells

Diallyl sulfide induces cell cycle arrest and apoptosis in HeLa human cervical cancer cells through the p53, caspase- and mitochondria-dependent pathways

Antioxidant properties of fruits of raspberry and blackberry grown in central Europe

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