Ellagic acid can act as a chaperone and suppress the heat-induced amyloid-like aggregation of ovalbumin

Yan, Chunjun; Zhou, Zheng

doi:10.1016/j.foodhyd.2019.105408

Cited by 21 publications

(6 citation statements)

References 64 publications

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“…The quenching constant K sv and the quenching rate constant K q at 25 °C were (1.58 ± 0.04) × 10 4 L mol –1 and (1.58 ± 0.04) × 10 12 L mol –1 s –1 , respectively. The value of K q is greater than the limiting diffusion rate constant of biomolecules (2 × 10 10 L mol –1 s –1 ), which indicates that the quenching mechanism of CAP on HL is static quenching . This quenching was due to the formation of a strong complex between CAP and HL.…”

Section: Resultsmentioning

confidence: 91%

“…The value of K q is greater than the limiting diffusion rate constant of biomolecules (2 × 10 10 L mol −1 s −1 ), which indicates that the quenching mechanism of CAP on HL is static quenching. 60 This quenching was due to the formation of a strong complex between CAP and HL. The modified Stern−Volmer plots are shown in Figure 10 C.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Attenuation of Human Lysozyme Amyloid Fibrillation by ACE Inhibitor Captopril: A Combined Spectroscopy, Microscopy, Cytotoxicity, and Docking Study

et al. 2021

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Misfolding proteins could form oligomers or amyloid fibers, which can cause a variety of amyloid-associated diseases. Thus, the inhibition of protein misfolding and fibrillation is a promising way to prevent and treat these diseases. Captopril (CAP) is an angiotensin-converting enzyme inhibitor (ACEI) that is widely used to treat diseases such as hypertension and heart failure. In this study, we found that CAP inhibits human lysozyme (HL) fibrillation through the combination techniques of biophysics and biochemistry. The data obtained by thioflavin-T (ThT) and Congo red (CR) assays showed that CAP hindered the aggregation of HL amyloid fibrils by reducing the β-sheet structure of HL amyloid, with an IC50 value of 34.75 ± 1.23 μM. Meanwhile, the particle size of HL amyloid decreased sharply in a concentration-dependent approach after CAP treatment. According to the visualization of atomic force microscopy (AFM) and transmission electron microscopy (TEM), we verified that in the presence of CAP, the needle-like fibers of HL amyloid were significantly reduced. In addition, CAP incubation dramatically improved the cell survival rate exposed to HL fibers. Our studies also revealed that CAP could form hydrogen bonds with amino acid residues of Glu 35 and Ala 108 in the binding pocket of HL, which help in maintaining the α-helical structure of HL and then prevent the formation of amyloid fibrillation. It can be concluded that CAP has antiamyloidogenic activity and a protective effect on HL amyloid cytotoxicity.

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Section: Resultsmentioning

confidence: 91%

Section: ■ Results and Discussionmentioning

confidence: 99%

Attenuation of Human Lysozyme Amyloid Fibrillation by ACE Inhibitor Captopril: A Combined Spectroscopy, Microscopy, Cytotoxicity, and Docking Study

et al. 2021

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“…Bacterial biofilms are composed of highly organized structures of bacterial cells and extracellular polymers, and usually require higher doses of antibiotics to kill the microbial cells in the biofilm and have stronger drug resistance. Yan & Zhou (2020a) and Yan & Zhou (2020b) studied the influence of EA on the growth of Streptococcus mutans and found that EA had an inhibitory effect on the formation of its bacterial biofilm, the mechanism of which may be through inhibiting the production of extracellular polymer, reducing bacterial adhesion, and destroying bacterial cell membrane, thereby reducing bacterial activity. Similarly, EA has been shown to inhibit the growth and reproduction of S. aureus by affecting its bacterial biofilm formation (Dürig et al, 2010).…”

Section: Bioactivity Of Ellagic Acidmentioning

confidence: 99%

“…EA, as a natural and effective polyphenolic component, belongs to polyphenolic dilactone, which is a dimer derivative of gallic acid (Yan & Zhou, 2020a; Yan & Zhou, 2020b). The main research paradigms of EA are in vitro cell culture and in vivo animal experiments, most of which are conducted by mouse models.…”

Section: Introductionmentioning

confidence: 99%

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The bioactivity and applications of pomegranate peel extract: A review

Xiang

Wen

et al. 2022

Journal of Food Biochemistry

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Pomegranate peel (PP) is a by-product in the processing of pomegranate products, which is usually discarded as a waste. However, a large number of researches have shown that pomegranate peel extract (PPE) is rich in a variety of phenolic substances, among which ellagic acid (EA), as one of the main active components, has significant biological activities, such as anti-oxidation, anti-tumor, anti-inflammatory, neuroprotection, anti-viral, and anti-bacterial. We analyzed the mechanism of EA's biological activity, and discussed its application in the food industry, for instance, food preservation, food additives, and functional foods. Combined with the research status of PPE, we discussed the limitations and development potential of PPE, in order to provide theoretical reference and scientific basis for the development and utilization of pomegranate by-products. Practical applicationsPomegranate peel (PP), the inedible part of the fruit, is usually treated as waste. In recent years, researchers have been committed to exploring various bioactive ingredients in PP and exploring its potential benefits to human health, which has far-reaching significance. In this paper, the chemical constituents of polyphenols in PP were reviewed, mainly focusing on the biological activity and mechanism of ellagic acid (EA).We reviewed the applications and invention patents of pomegranate peel extract (PPE) in food field, including food preservation, food additive, and functional foods, providing reference for the recycling and reuse of PP.

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Degradable cellulose/polylactic acid facial masks with antibacterial and antioxidant action from pomegranate extract

Wang

Liu

et al. 2023

Cellulose

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Ellagic acid can act as a chaperone and suppress the heat-induced amyloid-like aggregation of ovalbumin

Cited by 21 publications

References 64 publications

Attenuation of Human Lysozyme Amyloid Fibrillation by ACE Inhibitor Captopril: A Combined Spectroscopy, Microscopy, Cytotoxicity, and Docking Study

Attenuation of Human Lysozyme Amyloid Fibrillation by ACE Inhibitor Captopril: A Combined Spectroscopy, Microscopy, Cytotoxicity, and Docking Study

The bioactivity and applications of pomegranate peel extract: A review

Degradable cellulose/polylactic acid facial masks with antibacterial and antioxidant action from pomegranate extract

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