Elizabethan Acting.

Estimation of the Cloning Potential in Six Selected Genotypes of Purple Coneflower (Echinacea PurpureaL.)

“…this result suggests that, in purple coneflower, proliferation of axillary buds should be the method of choice for cloning. At present, plantlets of purple coneflower can be recovered in vitro in various ways (6,13,14,21), including somatic embryogenesis (6), however, cloning by axillary bud multiplication seems more natural and might entail a lesser risk of mutation (8).…”

“…Cultures for assessing the effects of BA concentrations on the proliferation of axillary buds Shoot tips, each with two or three tiny leaves, were isolated from plantlets growing on hormone-free medium and were transferred onto medium supplemented with various concentrations of BA (5,8). After 45 days of culture, the effects of BA concentrations on the proliferation of axillary buds were assessed by counting the number of buds proliferated from the shoot tip explants.…”

“…Due to the heterozygous nature of the seeds, plants growing from them would perform differently, causing difficulty in field management and uncertainty of the effects of the pharmaceutically functional products (4,18). On the other hand, cloning is an advanced technique through which a single plant or even a cell could be used for the production of hundreds of thousands of intact plantlets for cultivation within a relatively short time (5,8). Because plants produced by cloning are of the same genotype, cultivation of cloned plants could overcome the shortages in cultivation of purple coneflower by the traditional methods.…”