ELISA validation and determination of cut-off level for chloramphenicol (CAP) residues in shrimp and fish

Sarwer, Md. Golam; Rony, Md. Mahmudul Hasan; Sharmin, Mst S; Chowdhury, AR; Bhowmik, Shuva

doi:10.3126/on.v15i1-2.18789

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…The antibiotic drug chloramphenicol with molecular formular (C11H12CL2N2O5) and molecular weight (323.126g/mol) is one of the antibiotic drugs used widely in the farming of aquatic animals to treat diseases and increase productivity (Sarwer et al, 2017). It is used in most cases for prophylactic and therapeutic conditions in aquaculture because of its broad-spectrum activities against gram negative and gram-positive bacteria.…”

Section: Introductionmentioning

confidence: 99%

Histological Changes in Liver and Kidney of Clarias gariepinus (Burchell, 1822) Juvenile Exposed to Sub-lethal Doses of Chloramphenicol (CAP)

Nkwuda¹,

Awoke²,

Nwakpa³

2020

AquaSt

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A 70 days study was carried out on the effect of Chloramphenicol (CAP) on the liver and kidney tissues of Clarias gariepinus juveniles using static renewal bioassay techniques. Two hundred and seventy fish with mean length 7.39±39cm and mean weight 12.37±65g were exposed to sub lethal concentrations of chloramphenicol. Sublethal concentrations used were T1 (20 mg/l), T2 (40 mg/l), T3 (60 mg/l), T4 (80 mg/l) and T5 (100 mg/l) while T0 (0mg/l) served as the control. The liver and kidney were removed for histopathological examinations. Observed histopathological changes in the liver and kidney were intra-hepatic hemorrhage, hypertrophic hepatocytes, tissue degeneration, cytoplasmic vacoulation, cellular necrosis, occlusion of tubular lumen, expansion of glomerulus, reduction of Bowman's space. Effect was most severe at the highest concentration (100 mg/l). Observed abnormalities in the liver and kidney of Clarias gariepinus juveniles illustrate the suitability of Clarias gariepinus in toxicity trials of antibiotics.

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Section: Introductionmentioning

confidence: 99%

Histological Changes in Liver and Kidney of Clarias gariepinus (Burchell, 1822) Juvenile Exposed to Sub-lethal Doses of Chloramphenicol (CAP)

Nkwuda¹,

Awoke²,

Nwakpa³

2020

AquaSt

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“…Among the four Upazilas of Bhola district, highest number of ponds (11760) were found in Bhola Sadar which occupied 2352 acre area and the lowest number (4250) was found in Tazumuddin Upazila with an area of 1290 acre. Sarwer et al [6] found that the total area of pond was 0.67 acre in Subarnachar Upazila. Pond types: It was found that in study area, 57.30% ponds were used for fish culture, whereas 26.52% and 16.18% ponds were cultivable and derelict respectively (Table 2).…”

Section: Structural Characteristics Of Fish Pondsmentioning

confidence: 99%

Effect of Multi-Ownership on Pond Aquaculture Production in Bhola District, Bangladesh

Pall¹,

Mondal²,

Majumdar³

et al. 2018

Fish Aquac J

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The study was carried out to appraise the effect of multi-ownership on management system and present situation of pond aquaculture in Bhola district, Bangladesh. Data were collected by personal questionnaire interview from randomly selected 200 farmers at four Upazilas of Bhola district. Participatory rural appraisal (PRA) tools were applied for verification of the collected data. A total number of 32,877 ponds with an area of 8,061 acre were found in the study area. Both multi-ownership (72.5%) and single ownership (27.5%) were observed in management approach of the ponds. 57.3%, 26.52% and 16.18% of the ponds were cultured, cultural and derelict respectively in the study area. Among the cultured ponds, most of the ponds (90%) were used for poly culture, 8% for mono culture and only 2% ponds were for fish seed nursing purposes. Extensive (60%), improved traditional (25%) and semiintensive (15%) management practices were found in the study area. The study also revealed that farmers use both organic and inorganic fertilizers with an average dose of 850 Kg/acre and 44 Kg/acre respectively for single owner ponds and 560 Kg/acre and 24 kg/acre for multi-owner ponds. For fish production farmers found to be applied supplementary feed parallel with fertilizers in their ponds which facilitate annual average fish production as 1,810 kg/ acre. The average total cost of fish production was determined as BDT 49,221/acre/year and BDT 38,850/acre/year for single owner and multi-owner ponds respectively. In the study area highest amount of production cost was spent for feeding purpose 23.07% and 22% in single owner and multi owner ponds respectively. The study also revealed that the average profit of fish production was identified as BDT 54,050/acre/year in multi-owner ponds and BDT 80,229/acre/year in single owner ponds. So multi ownership of ponds has negative effect on the production of pond aquaculture in Bhola District.

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“…Standard solutions of Chloramphenicol, AOZ, AMOZ, AHD, and SEM were prepared from the stock solutions according to protocol provided (Sarwer et al, 2017). ELISA (Enzyme Linked Immunosorbent Assay) was applied to determine the level of antibiotic residues in the collected samples (Sarwer et al, 2017). AOZ (type R3703) and AMOZ (R3711), AHD (NF3463), SEM (NF3461) and ELISA kit with chloramphenicol were purchased.…”

Section: Standard Solutions Preparation For Elisamentioning

confidence: 99%

Determination of antibiotic residues in Black tiger shrimps (Penaeus monodon) and commercial shrimp feeds in Chattogram

Mahmood

Sultana

Mahmud

et al. 2021

Asian J. Med. Biol. Res

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Shrimps are good source of protein and also good exportable item for its quality all over the world. Inappropriate use of antibiotics in the production of local and export quality shrimps can facilitate infiltration of antibiotic residues into human bodies which are toxic enough to cause serious health hazard. The present study was investigated to determine the antibiotic residues in some selected local market and export quality shrimps and also commercial shrimp feeds in chattogram. Local grade and export grade samples of Black tiger shrimps (Penaeus monodon) and feeds of four different companies were taken for the completion of this study. The permissible residual limit for the shrimp samples were- 0.3(μg/kg) for Chloramphenicol and 1.0 (μg/kg) for AHD, AMOZ, SEM, AOZ. Black tiger shrimps from local market 1 had high concentration of Chloramphenicol (0.35 μg/kg) and SEM (1.2 μg/kg) residues whereas Market 2 showed maximum residues in AMOZ (1.2 μg/kg) and SEM (1.1 μg/kg). Whereas, Market 3 showed negative result. Local market 4 had high concentration of Chloramphenicol (0.4 μg/kg) and AOZ (1.2 μg/kg) residues. However, in export grade shrimps antibiotic residues was found under the maximum residual limit in all samples. Also, among four feed tested, two of them were found positive for chlorampenicol and nitrofurans. The result was considered significant where P>0.05. So, legal actions should be taken against the farmers and the farms that overuse antibiotics in shrimps. Also, people should be made aware of the harmful effects of antibiotics so that the impact can be minimized. Asian J. Med. Biol. Res. December 2020, 6(4): 623-627

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ELISA validation and determination of cut-off level for chloramphenicol (CAP) residues in shrimp and fish

Cited by 4 publications

References 6 publications

Histological Changes in Liver and Kidney of Clarias gariepinus (Burchell, 1822) Juvenile Exposed to Sub-lethal Doses of Chloramphenicol (CAP)

Histological Changes in Liver and Kidney of Clarias gariepinus (Burchell, 1822) Juvenile Exposed to Sub-lethal Doses of Chloramphenicol (CAP)

Effect of Multi-Ownership on Pond Aquaculture Production in Bhola District, Bangladesh

Determination of antibiotic residues in Black tiger shrimps (Penaeus monodon) and commercial shrimp feeds in Chattogram

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