ELISA using intact promastigotes for immunodiagnosis of kala-azar

Mohammed, El Amin El Roufaie; Wright, Edward; Kager, Piet A.; Laarman, J. J.; Pondman, K. W.

doi:10.1016/0035-9203(85)90376-1

Cited by 30 publications

(3 citation statements)

References 10 publications

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“…The prepared substrate solution (100 μL) was added to each well and incubated for 30 min at RT in the dark. It was then measured at 405 nm in ELISA ( 29 , 30 ).…”

Section: Methodsmentioning

confidence: 99%

Polymeric Approach to Adjuvant System in Antibody Production against Leishmaniasis Based on Hybridoma Technology

Yildiz¹,

Koken²,

Abamor³

et al. 2022

IJPA

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Background: Leishmaniasis is a zoonotic disease, which is one of the serious public health problems in the world. Nowadays, antibody production using hybridoma technology may be a correct approach in terms of sensitivity in the diagnosis of diseases such as leishmaniasis. The aim of this study was investigation of the effectiveness of different adjuvants on polyclonal antibody production against L. tropica based on hybridoma technique. Methods: Accordingly, Freund's adjuvant (1956, M. tuberculosis), as a classic adjuvant in studies, was used comparatively with the non-toxic polymeric based Polyoxidonium adjuvant. All animal immunization procedures were conducted at Bezm-i Alem University Experimental Animal Research Center. The adjuvant response was tested both in the serum sample and in the antibodies produced by the hybridomas. The antibody titers were determined with ELISA. Results: Freund’s and Polyoxidonium (PO) group blood titer’s increased approximately 5.5 fold compared to control after the 6th and 8th immunization. Hybridomas produced from mice immunized with PO adjuvant induced only antigen-specific antibody response and did not develop an immune response against the adjuvant. Conclusion: Adjuvant selection is very important in terms of the specificity of antibody responses of cells produced in hybridoma technology. Therefore, PO is recommended as a new adjuvant system in this study.

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“…The prepared substrate solution (100 μL) was added to each well and incubated for 30 min at RT in the dark. It was then measured at 405 nm in ELISA ( 29 , 30 ).…”

Section: Methodsmentioning

confidence: 99%

Polymeric Approach to Adjuvant System in Antibody Production against Leishmaniasis Based on Hybridoma Technology

Yildiz¹,

Koken²,

Abamor³

et al. 2022

IJPA

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“…For the diagnosis of leishmaniasis; serological methods such as enzyme-linked immunoassay (ELISA) test, western blot (WB), rk39 immunochromatographic diagnostic test (ICT), immunofluorescence antibody test (IFAT) are used. The basic principle of these methods is that they should be non-invasive methods based on the detection of antibodies or antigens against the parasite in the patient's serum or urine [95][96][97]. IFAT, which is a method based on serological and microscopy, provide investigation taken in different concentration serum sample of consisting IgG by staining the detected parasites with fluorescence to determine the presence of parasites.…”

Section: Diagnosticmentioning

confidence: 99%

Modern Aspects of Leishmaniasis: Basis of Development New Approaches against Infection

Pinar Zorba Yildiz,

Yildirim Koken,

Yavuz

et al. 2024

Current Topics in Zoonoses

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In this review, the basic principles of developing new approaches to leishmaniasis have been stated contrary to the available literature on Leishmania. In general, the morphology of parasites, life cycle, clinical forms, correspondence to epidemiology, and distribution according to species in the world were described. It has been expressed in various aspects of the interaction between host and parasite cell receptors. This plays an extremely important role in vaccine and drug development against leishmaniasis. Immunologically, natural immunocytes emphasize the importance of macrophages and dendritic cells in the Leishmania parasites’ immunopathology. This review will also discourse on the possibilities and necessity for the generation of new treatment technologies for leishmaniasis.

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“…However, these methods are very invasive, and their usage is limited to hospitals with good facilities [ 24 ]. An ELISA and direct agglutination test (DAT), which detects L. donovani -specific antibodies in serum, have been used as complementary to them [ 25 , 26 , 27 ]. The immunochromatographic card test (ICT) with a recombinant antigen rK39 (Kalazar Detect TM Rapid Test), which is derived from Leishmania chagasi kinesin-like protein, is sensitive and widely used for the diagnosis.…”

Section: Practical Examples Of Application Of Urine For Antibody Detection In Parasitic Diseasesmentioning

confidence: 99%

Detection of Urinary Antibodies and Its Application in Epidemiological Studies for Parasitic Diseases

et al. 2021

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For epidemiological studies of infectious diseases, pathogen-specific antibody levels in an area give us essential and appropriate information. The antibodies against pathogens are usually detected in blood, the drawing of which inconveniences people. Collection of blood increases the risk of accidental infections through blood, and it is difficult to obtain the participation of the target populations, especially the younger generation. On the other hand, urine samples, which contain a high enough level of antibodies for ELISA, can be harmlessly and easily collected and therefore have been used for epidemiological studies for diseases. The antibody examination of urine has been used for the epidemiology of parasitic diseases with a high sensitivity and specificity of serum samples. In this paper, we reviewed antibody assays with urine for seven parasitic diseases that urine diagnostic methods have reported in the past, and these are important infections included in NTDs, caused, for example, by Leishmania donovani, Wuchereria bancrofti, Schistosoma japonicum, Paragonimus westermani, Echinococcus granulosus, Echinococcus multilocularis, Strongyloides stercoralis, and Opisthorchis viverrini. The easy and safe urine surveillance system might be an admirable tool for future epidemiological studies for infectious diseases.

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ELISA using intact promastigotes for immunodiagnosis of kala-azar

Cited by 30 publications

References 10 publications

Polymeric Approach to Adjuvant System in Antibody Production against Leishmaniasis Based on Hybridoma Technology

Polymeric Approach to Adjuvant System in Antibody Production against Leishmaniasis Based on Hybridoma Technology

Modern Aspects of Leishmaniasis: Basis of Development New Approaches against Infection

Detection of Urinary Antibodies and Its Application in Epidemiological Studies for Parasitic Diseases

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