ELISA Test for Analyzing of Incidence of Type 1 Diabetes Autoantibodies (GAD and IA2) in Children and Adolescents

Delić-Šarac, Marina; Mutevelic, Selma; Karamehić, Jasenko; Subasic, Djemo; Jukić, Tomislav; Ćorić, Jozo; Ridjic, Ognjen; Panjeta, Mirsad; Žunić, Lejla

doi:10.5455/aim.2016.24.61-65

Cited by 12 publications

(9 citation statements)

References 22 publications

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“…Indeed, type 1A diabetes is an autoimmune disease due to the destruction of pancreatic islet β-cells by T cells. These results corroborate those of Delic-Sarac et al [10], who found anti-GAD and anti-IA2 autoantibodies in the diabetic patient's serum. The presence of these autoantibodies in siblings of T1D shows that they constitute a population at risk for diabetes, as Siljander et al [12], De Grijse et al [13] and Simonen-Tikka et al [20], showed in their study.…”

Section: Discussionsupporting

confidence: 92%

“…It is an incurable disease that is gaining momentum in Africa and particularly in Côte d'Ivoire. Today, the diagnosis of type 1A diabetes is biological (fasting glucose greater than 1.26 g / l) and requires the search for autoantibodies of diabetes as a confirmation marker for autoimmune process [8][9][10]. According to several authors, these autoantibodies of diabetes are present in siblings of type 1A diabetics (T1D) and are markers of the imminence of diabetes in this population at risk [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Age of Appearance of Diabetes Autoantibodies in Type 1a Diabetics and Their Siblings in Côte D'ivoire

Judith¹,

Mamadou²,

Pascal³

et al. 2018

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Diabetes autoantibodies, which are important markers of classification and prevention of type 1 diabetes, are absent in the diabetes care in Côte d'Ivoire. The study aim was to determine the age at which these autoantibodies were at their highest levels in type 1A diabetic patients (T1D) and their siblings in Côte d'Ivoire. The study population is composed of 28 T1D patients and 59 siblings, aged from 5 to 21 years. T1D were followed up in the U.H.C Endocrinology departments of Yopougon and Treichville, in the District of Abidjan. Anti-GAD and anti-IA2 autoantibodies were researched by ELISA and anti-ICA autoantibodies by indirect immunofluorescence on monkey pancreas. The peaks of anti-GAD and anti-IA2 autoantibodies were observed respectively at 9 years old (604.30±521.70 IU/ml) and 10 years old (125.10±90.10 IU/ml) in T1D. In siblings, the peaks of anti-GAD and anti-IA2 autoantibodies were observed respectively at 13 years old (137 IU/ml) and 10 years old (53±40 IU/ml). The peak of diabetes autoantibodies in T1D and their siblings in Côte d'Ivoire is at a very young age noted. This could explain the high mortality rate among T1D youth in Côte d'Ivoire.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Age of Appearance of Diabetes Autoantibodies in Type 1a Diabetics and Their Siblings in Côte D'ivoire

Judith¹,

Mamadou²,

Pascal³

et al. 2018

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“…With regard to anti-IA2 autoantibodies, the results showed a maximum value of 310 IU/ml in the first year of diabetes, followed by a brutal fall from the 2nd year, 5th, 6th until the 7th year (248.60 ± 95.40 IU/ml, 89.80 ± 25.78 IU/ml, 49.95 ± 39.05 IU/ml and 25.73 IU/ml). These results show that β-cells of Langerhans islets of the endocrine pancreas of these T1D know undergo a fast destruction, which can lead 7 to the death of these. The rebound of anti-GAD autoantibodies observed at the 7th year of diabetes and at the 9th year for anti-IA2 is thought to be due to the persistence of the humoral immune response against the islet cells of the Langherans of the pancreas.…”

Section: Discussionmentioning

confidence: 69%

“…It is an incurable disease that is gaining momentum in Africa and particularly in Côte d'Ivoire. Today, the diagnosis of type 1A diabetes is biological (fasting glucose greater than 1.26 g / l) and requires the search for autoantibodies of diabetes as a confirmation marker for autoimmune process [7]. According to some authors, these autoantibodies diabetes disappear with the duration of diabetes [8].…”

Section: Introductionmentioning

confidence: 99%

Progression of Autoantibodies Anti-Gad and Anti-Ia2 in Type 1a Diabetics Aged of 5 to 21 Years in Côte D’ivoire

Trébissou

Yapo-Crezoit

Pascal³

et al. 2019

Preprint

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Background: Diabetes autoantibodies are indispensable markers of diabetes classification.Objective: to research autoantibodies anti-GAD and anti-IA2 in type 1A diabetics (T1D) aged 5 to 21 years, and to follow the progression of these autoantibodies in T1D patients, in Côte d'Ivoire. Methods:The study population composed of 28 T1D patients, aged 5 to 21 years. T1D were followed up in two diabetes care centers in Abidjan district, Endocrinology departments of U.H.C of Yopougon and Treichville. Anti-GAD and anti-IA2 autoantibodies were researched by ELISA.Results: anti-GAD and anti-IA2 were present in T1D and their siblings. After 2 years of diabetes, the titer of the anti-GAD autoantibodies increased to the mean value of 677.10 ± 353.20 IU / ml. Then, a fall of the anti-GAD autoantibodies until the cancellation was observed from the 8th to the 9th with values of 117 IU / ml to 10.14 IU / ml. Anti-IA2 autoantibodies fall at 9th year of diabetes with a value of 55.10 IU / ml. Conclusion:anti-GAD and anti-IA2 autoantibodies persist after 9 years of diabetes, causing total destruction over time of the pancreatic β-cell mass in patients from Côte d'Ivoire, leading them to the death.

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“…For the control group, to rule out the diagnosis of T1D, we conducted a GAD 65 assay. GAD 65 assay was conducted by using an indirect enzyme-linked immunoabsorbent assay (ELISA) method, as previously described by literature [22]. Nutritional status was defined by plotting body mass index (BMI) into a standardized WHO chart for children aged 10-19 years old, then categorized as obese, overweight, normal, and underweight.…”

Section: Methodsmentioning

confidence: 99%

Role of Vitamin D in Premature Atherosclerosis in Adolescents Type 1 Diabetes through Transforming Growth Factor-β1, Interferon-γ, Interleukin-10, and Interleukin-17

Cahyono

Barlıanto

Handayani

et al. 2020

Open Access Maced J Med Sci

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BACKGROUND: Cardiovascular disease (CVD) is one the cause of mortality in patients with type 1 diabetes (T1D). The development of CVD is mainly triggered by atherosclerosis, which is associated with the inflammatory process. AIM: The current study was aimed to investigate the association of Vitamin D level and premature atherosclerosis in adolescents with T1D, mainly through the regulation of various cytokines (interferon-γ [IFN-γ], IL-17, interleukin-10 [IL-10], and transforming growth factor-β1 [TGF-β1]). METHODS: This study was designed as a cross-sectional study involving 40 T1D and 40 healthy control who came to the outpatient clinic, Saiful Anwar Hospital, Malang, Indonesia, within the study period (January 2019-July 2019). RESULTS: Our data demonstrated that the IFN-γ and IL-17 levels were significantly higher (p < 0.001), whereas the TGF-β1 and IL-10 levels were significantly lower (p < 0.001) in T1D group compared with control. Furthermore, T1D also has higher carotid intima-media thickness (cIMT) value and lower flow-mediated dilatation (FMD) value compared to the control group (p < 0.001). Level of 25(OH)D3 was strongly associated with reduced cIMT and elevated FMD (p < 0.005). The direct effect of 25(OH)D3 on cIMT and FMD was higher than the indirect effect of Vitamin D through TGF-β1, IL-10, IL-17, and IFN-γ. The cutoff value of 25(OH)D3 levels for the risk of atherosclerosis was 12.8 ng/dL (sensitivity 85.7% and specificity 86.7%). CONCLUSION: The level of Vitamin D in the T1D group was significantly lower than those in healthy children and Vitamin D deficiency substantially influences the formation of premature atherosclerosis.

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ELISA Test for Analyzing of Incidence of Type 1 Diabetes Autoantibodies (GAD and IA2) in Children and Adolescents

Cited by 12 publications

References 22 publications

Age of Appearance of Diabetes Autoantibodies in Type 1a Diabetics and Their Siblings in Côte D'ivoire

Age of Appearance of Diabetes Autoantibodies in Type 1a Diabetics and Their Siblings in Côte D'ivoire

Progression of Autoantibodies Anti-Gad and Anti-Ia2 in Type 1a Diabetics Aged of 5 to 21 Years in Côte D’ivoire

Role of Vitamin D in Premature Atherosclerosis in Adolescents Type 1 Diabetes through Transforming Growth Factor-β1, Interferon-γ, Interleukin-10, and Interleukin-17

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