ELISA for the detection of venom antigens in experimental and clinical envenoming by Loxosceles intermedia spiders

Chávez-Olórtegui, Carlos; Zanetti, Vera C; Ferreira, Ana Paula; Minozzo, João Carlos; Mangili, Oldemir C.; Gubert, Ida Cristina

doi:10.1016/s0041-0101(97)00159-1

Cited by 30 publications

(14 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the early stages, cutaneous loxoscelism may be misdiagnosed as cellulitis or an insect sting. Although incipient laboratory tests have been perfomed 8,9 , no definitive immunodiagnosis is yet available.…”

mentioning

confidence: 99%

Clinical and epidemiological features of definitive and presumed loxoscelism in São Paulo, Brazil

Málaque

Castro-Valencia

Cardoso

et al. 2002

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

A retrospective study analysed 359 proven or presume cases of loxoscelism seen at the Hospital Vital Brazil, Instituto Butantan, São Paulo, Brazil, between 1985 and 1996. The spider was identified in 14%. The bites occurred predominantly in the urban areas (73%) between September and February. Patients > 14 years were commonest inflicted (92%) and 41% were bitten while getting dressed. Only 11% sought medical care within the first 12 hours post bite. Cutaneous loxoscelism was the commonest form presenting (96%); commonest manifestations were: pain (76%), erythema (72%), edema with enduration (66%), ecchymosis (39%). Skin necrosis occurred in 53% of patients, most frequently seen on trunk, tigh and upper arm, and when patients seek medical care more than 72 hours after bite. Local infection was detected in 12 patients (3%). Hemolysis was confirmed in 4 cases (1.1%). Generalised cutaneous rash, fever and headache were also observed in 48% of the total of patients. None of them had acute renal failure or died. Treatment usually involved antivenom administration (66%), being associated with corticosteroids (47%) or dapsone (30%). Presumptive diagnosis of loxoscelism may be established based on clinical and epidemiological findings. Further investigations are required to prove the value of antivenom and other treatment schedules.

show abstract

mentioning

confidence: 99%

Clinical and epidemiological features of definitive and presumed loxoscelism in São Paulo, Brazil

Málaque

Castro-Valencia

Cardoso

et al. 2002

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

show abstract

“…South American investigators have developed an ELISA for the detection of venom antigens in experimental envenomation by the South American spider L inter-media. 29 The ELISA modestly cross-reacted against other South American Loxosceles species spiders (L gaucho and L laeta) and against a variety of snake species. The South American ELISA has not been tested against North American venoms.…”

Section: Discussionmentioning

confidence: 94%

“…The South American ELISA has not been tested against North American venoms. 29 A passive hemagglutination inhibition test for the diagnosis of North American brown recluse spider bites was reported in 1993. 30 The passive hemagglutination test is sensitive; however, it is cumbersome to prepare, and the results are available only after several (6 to 24) hours.…”

Section: Discussionmentioning

confidence: 99%

A new assay for the detection of Loxosceles species (brown recluse) spider venom

Gomez

Krywko

Stoecker

2002

Annals of Emergency Medicine

View full text Add to dashboard Cite

Study objective-Dermal lesions from unrelated arthropod species and medical causes appear similar to Loxosceles species (brown recluse spider) bites. This may result in delayed diagnosis and treatment. We developed a sensitive Loxosceles species venom enzyme-linked immunosorbent assay (ELISA) and characterized the specificity of the assay by evaluating antigenic cross-reactivity from a variety of North American arthropod venoms. Methods-NorthAmerican arthropod (14 spiders, 2 scorpions, and 1 bee) venoms were studied. Three venom amounts (diluted in 100 μL of ELISA buffer) were assayed: 16,000 ng, 2,000 ng, and 40 ng. The latter quantity was selected because this is the observed maximum amount of venom we detect when inoculating dermis with amounts likely to be deposited by a spider bite. The larger venom amounts are overwhelming quantities designed to test the limits of the assay for arthropod venom cross-reactivity. Similar amounts of Loxosceles species venom and bovine albumin served as positive and negative controls, respectively.Results-At the lowest amount of venom tested (40 ng), the ELISA detected only the Loxosceles species positive control. When 2,000 ng was assayed, only Scytodes fusca and Kukulcania hibernalis arachnid venoms (in addition to Loxosceles species) cross-reacted to the assay. Finally, at 16,000 ng, the ELISA assay modestly detected Diguetia canities, Heteropoda venatoria, Tegenaria agrestis, Plectreurys tristes, Dolomedes tenebrosus, and Hadrurus arizonensis arachnid venoms.Conclusion-Cross-reactivity was observed in 8 of 17 North American arthropod venoms when large venom amounts were assayed with a Loxosceles species ELISA. By using a relevant quantity of venom, 40 ng, the assay was specific for Loxosceles species venom. The venom specificity of the ELISA may allow clinical application in Loxosceles species endemic regions of North America.

show abstract

“…74 The presence of venom from patient lesions and sera can be detected using enzyme-linked immunosorbent assay in Brazil, and although it demonstrates high specificity and sensitivity, is not in widespread clinical use. 58,75 Another promising Loxosceles venom-specific immunoassay that is under investigation uses hair, skin biopsies, or aspirated tissue near a suspected lesion to detect the presence of venom up to 7 days postinjury using a venom-specific enzyme immunoassay. 76,77 In the viscerocutaneous manifestation of envenomation, some laboratory and clinical characteristics can be helpful in determining its presence and severity.…”

Section: Laboratory Diagnosismentioning

confidence: 99%

Loxoscelism: Old obstacles, new directions

Hogan

Barbaro²,

Winkel³

2004

Annals of Emergency Medicine

158

105

View full text Add to dashboard Cite

ELISA for the detection of venom antigens in experimental and clinical envenoming by Loxosceles intermedia spiders

Cited by 30 publications

References 9 publications

Clinical and epidemiological features of definitive and presumed loxoscelism in São Paulo, Brazil

Clinical and epidemiological features of definitive and presumed loxoscelism in São Paulo, Brazil

A new assay for the detection of Loxosceles species (brown recluse) spider venom

Loxoscelism: Old obstacles, new directions

Contact Info

Product

Resources

About