1986
DOI: 10.1021/ac00121a026
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Elimination of quenching effects in luminescence spectrometry by phase resolution

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Cited by 11 publications
(2 citation statements)
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“…The preexponential factor (A) can provide a measure of analyte concentration with very good detection limits even in the presence of quenching and scattering or fluorescence errors. When the sample is excited with a short-duration light pulse, the preexponential factor is directly related to the concentration of the luminescent species and is independent of quenching errors (4,5).…”
mentioning
confidence: 99%
“…The preexponential factor (A) can provide a measure of analyte concentration with very good detection limits even in the presence of quenching and scattering or fluorescence errors. When the sample is excited with a short-duration light pulse, the preexponential factor is directly related to the concentration of the luminescent species and is independent of quenching errors (4,5).…”
mentioning
confidence: 99%
“…The method even tolerates low levels of ambient light and displacements in the optical setup in the time period between calibration and actual measurements. 93,99 In contrast to the DLR method, the rapid lifetime determination is not affected by effects of photobleaching. Even if the fluorophore has lost 50% of its luminescence intensity due to bleaching, the relative intensities in the two gates A 1 and A 2 are reduced to the same extent.…”
Section: Rapid Lifetime Determination (Rld)mentioning
confidence: 99%