Elimination of polyamine N-acetylation and regulatory engineering improved putrescine production by Corynebacterium glutamicum

Nguyen, Anh Q.; Schneider, Jens; Wendisch, Volker F.

doi:10.1016/j.jbiotec.2014.10.035

Cited by 59 publications

(69 citation statements)

References 54 publications

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“…As the deletion of cgmR in C. glutamicum wild type decreased the expression of the L-arginine biosynthesis genes (Nguyen et al 2015), we hypothesized that the predicted cgmARcg2895 operon may influence L-arginine production. Since cgmA and cg2895 encode permeases of the major facilitator superfamily, and cgmA overexpression was shown to increase putrescine (Nguyen et al 2015) and cadaverine production (Kind et al 2011), the effect of cgmA and cg2895 overexpression on L-arginine accumulation by L-arginine-producing strains was determined.…”

Section: Resultsmentioning

confidence: 99%

“…Kan R , pk19mobsacB with the deletion construct of gene cgmR (cg2894) (Nguyen et al 2015) pK19ΔcgmOp…”

Section: Determination Of Amino Acid Concentrationsmentioning

confidence: 99%

“…The major facilitator superfamily (MFS) permease CgmA may be involved in export of the diamine putrescine (Nguyen et al 2015) and cadaverine (Kind et al 2011), and overexpression of cgmA increased putrescine and cadaverine titers. The gene cgmA is part of the predicted cgmAR-cg2895 operon that is regulated by the TetR-like repressor CgmR (Itou et al 2005) in a diamine-dependent manner (Kind et al 2011) as shown by gel shift analysis of purified CgmR (Nguyen et al 2015). Deletion of cgmR was shown to improve cadaverine production (Kind et al 2011) and putrescine production (Nguyen et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…The gene cgmA is part of the predicted cgmAR-cg2895 operon that is regulated by the TetR-like repressor CgmR (Itou et al 2005) in a diamine-dependent manner (Kind et al 2011) as shown by gel shift analysis of purified CgmR (Nguyen et al 2015). Deletion of cgmR was shown to improve cadaverine production (Kind et al 2011) and putrescine production (Nguyen et al 2015). A DNA microarray analysis of differential gene expression in the absence of CgmR revealed decreased RNA levels of the L-arginine biosynthesis genes and increased RNA levels of the L-arginine repressor gene argR (Nguyen et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Deletion of cgmR was shown to improve cadaverine production (Kind et al 2011) and putrescine production (Nguyen et al 2015). A DNA microarray analysis of differential gene expression in the absence of CgmR revealed decreased RNA levels of the L-arginine biosynthesis genes and increased RNA levels of the L-arginine repressor gene argR (Nguyen et al 2015). This prompted us to study if the cgmAR-cg2895 operon is not only relevant for diamine production but may also play a role in production of L-arginine and other amino acids.…”

Section: Introductionmentioning

confidence: 99%

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Roles of export genes cgmA and lysE for the production of l-arginine and l-citrulline by Corynebacterium glutamicum

Lubitz

Jorge

Pérez-García

et al. 2016

Appl Microbiol Biotechnol

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L-arginine is a semi-essential amino acid with application in cosmetic, pharmaceutical, and food industries. Metabolic engineering strategies have been applied for overproduction of L-arginine by Corynebacterium glutamicum. LysE was the only known L-arginine exporter of this bacterium. However, an L-arginine-producing strain carrying a deletion of lysE still accumulated about 10 mM L-arginine in the growth medium. Overexpression of the putative putrescine and cadaverine export permease gene cgmA was shown to compensate for the lack of lysE with regard to L-arginine export. Moreover, plasmid-borne overexpression of cgmA rescued the toxic effect caused by feeding of the dipeptide Arg-Ala to lysE-deficient C. glutamicum and argO-deficient Escherichia coli strains. Deletion of the repressor gene cgmR improved L-arginine titers by 5 %. Production of L-lysine and L-citrulline was not affected by cgmA overexpression. Taken together, CgmA may function as an export system not only for the diamine putrescine and cadaverine but also for L-arginine. The major export system for L-lysine and L-arginine LysE may also play a role in L-citrulline export since production of L-citrulline was reduced when lysE was deleted and improved by 45 % when lysE was overproduced.

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Section: Resultsmentioning

confidence: 99%

“…Kan R , pk19mobsacB with the deletion construct of gene cgmR (cg2894) (Nguyen et al 2015) pK19ΔcgmOp…”

Section: Determination Of Amino Acid Concentrationsmentioning

confidence: 99%