Elimination of Microflora From Barley Kernels With Ethylene Oxide

Bushnell, W. R.

doi:10.4141/cjps73-007

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…The second possible theory for the enhanced antimicrobial effect of AgNO 3 due to soaking is that during soaking, dormant microorganisms become more active and subsequently more susceptible to stress. Both pathways for the efficiency-inducing effect of moisture have been proposed earlier to explain the increased antimicrobial effect of ethylene and propylene oxide when relative humidity of barley grains increased [40].…”

Section: Discussionmentioning

confidence: 75%

AgNO3 Sterilizes Grains of Barley (Hordeum vulgare) without Inhibiting Germination—A Necessary Tool for Plant–Microbiome Research

Munkager

Vestergård

Priemé

et al. 2020

Plants

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To understand and manipulate the interactions between plants and microorganisms, sterile seeds are a necessity. The seed microbiome (inside and surface microorganisms) is unknown for most plant species and seed-borne microorganisms can persist and transfer to the seedling and rhizosphere, thereby obscuring the effects that purposely introduced microorganisms have on plants. This necessitates that these unidentified, seed-borne microorganisms are removed before seeds are used for studies on plant-microbiome interactions. Unfortunately, there is no single, standardized protocol for seed sterilization, hampering progress in experimental plant growth promotion and our study shows that commonly applied sterilization protocols for barley grains using H 2 O 2 , NaClO, and AgNO 3 yielded insufficient sterilization. We therefore developed a sterilization protocol with AgNO 3 by testing several concentrations of AgNO 3 and added two additional steps: Soaking the grains in water before the sterilization and rinsing with salt water (1% (w/w) NaCl) after the sterilization. The most efficient sterilization protocol was to soak the grains, sterilize with 10% (w/w) AgNO 3 , and to rinse with salt water. By following those three steps, 97% of the grains had no culturable, viable microorganism after 21 days based on microscopic inspection. The protocol left small quantities of AgNO 3 residue on the grain, maintained germination percentage similar to unsterilized grains, and plant biomass was unaltered. Hence, our protocol using AgNO 3 can be used successfully for experiments on plant-microbiome interactions.

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Section: Discussionmentioning

confidence: 75%

AgNO3 Sterilizes Grains of Barley (Hordeum vulgare) without Inhibiting Germination—A Necessary Tool for Plant–Microbiome Research

Munkager

Vestergård

Priemé

et al. 2020

Plants

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Simultane, gas- chromatographische Bestimmung von �thylenoxid, �thylenchlorhydrin und �thylenglykol in Getreide

Pfeilsticker

Fabricius

Timme

1975

Z Lebensm Unters Forch

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The not comminuted grain (10 g) is extracted for 24 hrs (25 degrees C) with 5 ml methanol (shaking machine), then the fluid phase is separated by centrifugal filtration and directly injected into the GC (10 mu). The GC starting temperature (60 degrees C) is adjusted by cooling the oven with Co2. Yield: EO 73% (concentr. range 25 ppm); ECH 93% (concentr. r. 50 ppm); EG 99% (conc. r. 50 ppm). Standard deviation: EO s = +/- 1,70 ppm (N = 24, conc. r. 10 ppm), ECH s = +/- 1,15 ppm (N = 24, conc. r. 20--30 ppm), EG s = +/- 0,62 ppm, (conc. r. 15--30 ppm, N = 24).

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Elimination of Microflora From Barley Kernels With Ethylene Oxide

Cited by 2 publications

References 10 publications

AgNO3 Sterilizes Grains of Barley (Hordeum vulgare) without Inhibiting Germination—A Necessary Tool for Plant–Microbiome Research

AgNO3 Sterilizes Grains of Barley (Hordeum vulgare) without Inhibiting Germination—A Necessary Tool for Plant–Microbiome Research

Simultane, gas- chromatographische Bestimmung von �thylenoxid, �thylenchlorhydrin und �thylenglykol in Getreide

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