Elimination of antibiotic resistance genes and human pathogenic bacteria by earthworms during vermicomposting of dewatered sludge by metagenomic analysis

Huang, Kui; Xing, Hui; Zhang, Yingying; Li, Jianhui; Cui, Guangyu; Li, Fusheng; Bai, Wei; Jiang, Yufeng; Wu, Nan

doi:10.1016/j.biortech.2019.122451

Cited by 80 publications

(20 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results highlight how deeply vermicomposting modi es bacterial and fungal microbiotas of sewage sludge and demonstrates the critical effect of earthworm gut associated processes. These changes of the microbiota produced by earthworm activity were postulated as the main reasons for the mitigation of antibiotic resistance genes (ARGs) during vermicomposting of sewage sludge [23][24][25] .…”

Section: Resultsmentioning

confidence: 99%

Earthworms drastically change fungal and bacterial communities during vermicomposting of sewage sludge

Domínguez

Aira

Crandall

et al. 2021

Preprint

View full text Add to dashboard Cite

Wastewater treatment plants produce hundreds of million tons of sewage sludge every year all over the world. Vermicomposting is well established worldwide and has been successful in processing sewage sludge, which can contribute to alleviate the severe environmental problems caused by their disposal. Here, we utilized 16S and ITS rRNA high-throughput sequencing to characterize bacterial and fungal community composition and structure during the gut- and cast-associated processes (GAP and CAP, respectively) of vermicomposting of sewage sludge. Bacterial and fungal community composition and diversity changed significantly during both vermicomposting processes. Most of the bacterial and fungal taxa in the sewage sludge were eliminated during vermicomposting, mainly through the GAP. Vermicomposting of sewage resulted in a stable and rich microbial community with potential biostimulant properties that may aid plant growth. Our results support the use of vermicompost derived from sewage sludge for sustainable agricultural practices.

show abstract

Section: Resultsmentioning

confidence: 99%

Earthworms drastically change fungal and bacterial communities during vermicomposting of sewage sludge

Domínguez

Aira

Crandall

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Similarly, the Simpson index showed a similar trend in both treatments. Earthworms can enrich the diversity of the bacterial community in vermicompost after vermicomposting, as determined using the DGGE method [4], PLFA method [31], high-throughput sequencing method [12,13], and metagenomic sequencing [14]. Herein, although the Shannon index increased after vermicomposting, earthworms did not significantly enhance the α diversity of bacteria compared with the control.…”

Section: Changes In Active Bacterial and Eukaryotic Diversitymentioning

confidence: 88%

“…Carbon and nitrogen analyzer (Multi N/C 2100, Jena, Germany) was used to determine dissolved organic carbon (DOC). Moreover, the DOC structure was analyzed via three-dimensional emission and excitation modes (3D-EEM) using a fluorescence spectrophotometer (RF-5300PC, SHIMADZU, Shanghai, China), as described by Huang [14].…”

Section: Assay Of Physicochemical Propertiesmentioning

confidence: 99%

“…Earthworms can decompose various excess sludges, such as primary sedimentation tank sludge [7], anaerobic digestion sewage sludge [8,9], and dewatered sludge [4,[10][11][12][13]. The final sludge vermicompost contains homogeneous particle with available nitrogen, phosphorus, potassium, micro-nutrients, and diverse microbial flora [5,14]. Therefore, the sludge vermicompost can be an agricultural organic fertilizer.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fate of Functional Bacterial and Eukaryotic Community Regulated by Earthworms during Vermicomposting of Dewatered Sludge, Studies Based on the 16S rDNA and 18S rDNA Sequencing of Active Cells

Yang

Huang

Peng

et al. 2021

IJERPH

Self Cite

View full text Add to dashboard Cite

DNA sequencing of active cells involved in vermicomposting can clarify the roles of earthworms in regulating functional microorganisms. This study aimed to investigate the effect of earthworms on functional microbial communities in sludge by comparing biodegradation treatments with and without earthworms. PCR and high throughput sequencing based on pretreatment of propidium monoazide (PMA) were used to detect the changes in active bacterial 16S rDNA and eukaryotic 18S rDNA during vermicomposting. The results showed that the nitrate in sludge vermicomposting and control were significantly different from day 10, with a more stable product at day 30 of vermicomposting. Compared with the control, the Shannon indexes of active bacteria and eukaryotes decreased by 1.9% and 31.1%, respectively, in sludge vermicompost. Moreover, Proteobacteria (36.2%), Actinobacteria (25.6%), and eukaryotic Cryptomycota (80.3%) were activated in the sludge vermicompost. In contrast, the control had Proteobacteria (44.8%), Bacteroidetes (14.2%), Cryptomycota (50.00%), and Arthropoda (36.59%). Network analysis showed that environmental factors had different correlations between active bacterial and eukaryotic community structures. This study suggests that earthworms can decrease the diversity of bacterial and eukaryotic communities, forming a specific-functional microbial community and thus accelerating organic matter decomposition during vermicomposting of dewatered sludge.

show abstract

“…In recent times, vermicomposting has been used to attenuate the release of ARG and human pathogenic bacteria (HPB), present in organic wastes, such as excess activated sludge (EAS) and some animal manures. Huang et al [84] found that worms decreased the abundance of ARG and HPB, but sludge vermicompost was still ARG and HPB enriched. Cui et al [85] studied the influence of vermicomposting on the ARG present in EAS, finding that its attenuation varied between 31%-99%, depending on the type of tested genes.…”

Section: Compost Vermicompost and Biocharmentioning

confidence: 99%

Natural Organic Compounds for Application in Organic Farming

2020

View full text Add to dashboard Cite

Chemical fertilizers, pesticides, and fungicides are widely used in agriculture to improve crop yields. Most of the compounds used are synthetic, and their overuse causes environmental pollution and human health problems. Currently, several countries are working to reduce the use of agrochemicals. Organic agriculture is now emerging as a sustainable alternative to traditional agriculture using environmentally friendly strategies such as the application of organic fertilizers from plant and animal waste and pesticides based on plant extracts and microbials. However, the availability of commercial biopesticides and organic fertilizers is very limited because there are certain barriers to the commercialization of biological products. These barriers include small available quantities of raw materials and strict registration laws requiring toxicological tests and other studies that are expensive and time consuming. The objective of this review is to provide details about the various organic fertilizers and pesticides that do not have the same disadvantages as synthetic compounds in terms of persistence and toxicity.

show abstract

Elimination of antibiotic resistance genes and human pathogenic bacteria by earthworms during vermicomposting of dewatered sludge by metagenomic analysis

Cited by 80 publications

References 40 publications

Earthworms drastically change fungal and bacterial communities during vermicomposting of sewage sludge

Earthworms drastically change fungal and bacterial communities during vermicomposting of sewage sludge

Fate of Functional Bacterial and Eukaryotic Community Regulated by Earthworms during Vermicomposting of Dewatered Sludge, Studies Based on the 16S rDNA and 18S rDNA Sequencing of Active Cells

Natural Organic Compounds for Application in Organic Farming

Contact Info

Product

Resources

About