Eliminatinof the temperature-induced loss of the enantioselectivity of chemically bonded albumin

Chiral Separations in Capillary Electrophoresis

“…The following aspects must be considered: (i) hydrogen bonding, (ii) hydrophobic interactions, (iii) charge effects, and (iv) chiral selector conformation effects. As demonstrated by the example of tryptophan separation, 186 the effect of a preliminary treatment of the selector by a pure organic solvent may also be of importance.…”

“…61,184 Consequently, the conformation of the selector may need to be adjusted to the needs of a particular separation or even in some cases be restored if needed. 186 The mechanism theory for chiral discrimination by albumins 79 utilizes knowledge from both liquid chromatography and electrophoresis. The conformational variability of albumins is considered the most significant factor in their ability to discriminate specific compounds and classes of compounds.…”

Chiral separations in capillary electrophoresis

“…The chemical composition of the separation system, its pH, temperature, and method of preparation of the selector affect its actual conformation in the system, and, as a result, the separation selectivity of the system. The conformational variability is evidently an additional and powerful tool for manipulation of the separation selectivity [85] which singles out the affinity chiral selectors from the other selector types. The conformational variability of affinity chiral selectors is responsible, with high probability, for many incompatible and even contradictory results which may be found in both chromatographic and electrophoretic communications dealing with these selectors.…”

Chiral separations by capillary zone electrophoresis: Present state of the art

“…However, variability of protein composition together with influence of their steric structure, as proved by the example of albumins [126], are the reasons for differences in the capability of various proteins to discriminate between enantiomers of particular compounds.…”

“…Vagueness about the proposed idea on chiral recognition by albumins [126] illustrates how fragmentary our knowledge is about processes resulting in chiral discrimination by so-called affinity interactions. From this viewpoint, the whole complex of studies to date on chiral discrimination by proteins has only serve das an introduction.…”