Eliminating transmissibility of bovine spongiform encephalopathy by dry-heat treatment

Matsuura, Yuichi; Ishikawa, Yukiko; Murayama, Yuichi; Yokoyama, Takashi; Somerville, Robert A.; Kitamoto, Tetsuyuki; Mohri, Satoshi

doi:10.1099/jgv.0.001335

Cited by 8 publications

(4 citation statements)

References 34 publications

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“…While extreme conditions are recommended for inactivation of prions by autoclaving (134 • C, 18 min) [6], transmissibility of some prions has been shown to remain even after dry-heating at temperatures of 400 • C [7]. In addition, treatment with sodium dodecyl sulfate (SDS), sodium hydroxide (NaOH), and sodium hypochlorite is known to inactivate prions [8][9][10], but these chemicals are generally impractical because of their corrosiveness [8].…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Scrapie Prions by the Electrically Charged Disinfectant CAC-717

Sakudo

Iwamaru

Furusaki³

et al. 2020

Pathogens

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Previous studies have revealed that the electrically charged disinfectant CAC-717 has strong virucidal and bactericidal effects but is safe for humans and animals. In this study, CAC-717 was further evaluated for its potential effects as a disinfectant against scrapie prions. Western blotting showed that CAC-717 reduced the amount of the abnormal isoform of prion protein (PrPSc) in prion-infected cell (ScN2a) lysates. Furthermore, the reduction of prion transmissibility was confirmed by a mouse bioassay, in which mice injected with scrapie prions pre-treated with CAC-717 survived longer than those injected with untreated scrapie prions. Lastly, to evaluate the seeding activity of ScN2a cell lysates treated with CAC-717, quantitative protein misfolding cyclic amplification (PMCA) was performed directly on ScN2a cell lysates treated with CAC-717, which showed that the median dose of PMCA (PMCA50) dropped from log9.95 to log5.20 after CAC-717 treatment, indicating more than a 4 log reduction. This suggests that the seeding activity of PrPSc is decreased by CAC-717. Collectively, these results suggest that CAC-717 has anti-prion activity, reducing both PrPSc conversion activity and prion transmissibility; thus, CAC-717 will be useful as a novel disinfectant in prion diseases.

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Section: Introductionmentioning

confidence: 99%

Inactivation of Scrapie Prions by the Electrically Charged Disinfectant CAC-717

Sakudo

Iwamaru

Furusaki³

et al. 2020

Pathogens

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“…It remains to be seen if these conditions are applicable to other proteins in prion form. Previous surface decontamination investigations reported that mammalian PrP retained functionality after exposure to 360°C for an hour and even following incineration at 600°C for 15 min ( Brown et al, 2000 ), however another work indicated that dry-heating at temperatures of 600°C and higher (but not 400°C or lower) resulted in inactivation of the PrP-based prion agent of bovine spongiform encephalopathy ( Matsuura et al, 2020 ). However, it is important to highlight that in these experiments, brain material was subjected to heat treatment.…”

Section: Discussionmentioning

confidence: 99%

Heat inactivation of stable proteinaceous particles for future sample return mission architecture

et al. 2022

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The National Aeronautics and Space Administration (NASA) and the European Space Agency (ESA) are studying how to improve the safety of future planetary science sample return missions that would bring back materials to Earth. Backward planetary protection requirements have been identified as a critical technology development focus in order to reduce the possibility of harm to Earth’s biosphere from such returned materials. In order to meet these challenges, NASA has identified the need for an appropriate suite of biological indicators (BIs) that would be used to develop, test, and ultimately validate sample return mission sterilization systems. Traditionally, BIs are defined as test systems composed of viable microorganisms that are inactivated when necessary conditions are met during sterilization procedures, providing a level of confidence in the process. BIs used traditionally at NASA have been driven by past mission requirements, mainly focused on spore-formers. However, spore-based BIs are insufficient as the only analog for a nominal case in sample return missions. NASA has directed sample return missions from habitable worlds to manage “potential extraterrestrial life and bioactive molecules” which requires investigation of a range of potential BIs. Thus, it is important to develop a mitigation strategy that addresses various known forms of biology, from complex organisms to biomolecular assemblies (including self-perpetuating non-nucleic acid containing structures). The current effort seeks to establish a BI that would address a stable biomolecule capable of replication. Additional engineering areas that may benefit from this information include applications of brazing, sealing, and impact heating, and atmospheric entry heating. Yeast aggregating proteins exhibit aggregation behavior similar to mammalian prion protein and have been successfully employed by researchers to understand fundamental prion properties such as aggregation and self-propagation. Despite also being termed “prions,” yeast proteins are not hazardous to humans and can be used as a cost effective and safer alternative to mammalian prions. We have shown that inactivation by dry heat is feasible for the prion formed by the yeast Sup35NM protein, although at higher temperature than for bacterial spores.

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“…10.3389/fmicb. 2022.911091 Frontiers in Microbiology 07 frontiersin.org decontamination investigations reported that mammalian PrP retained functionality after exposure to 360°C for an hour and even following incineration at 600°C for 15 min (Brown et al, 2000), however another work indicated that dry-heating at temperatures of 600°C and higher (but not 400°C or lower) resulted in inactivation of the PrP-based prion agent of bovine spongiform encephalopathy (Matsuura et al, 2020). However, it is important to highlight that in these experiments, brain material was subjected to heat treatment.…”

Section: Dry Heat As a Tool For Prion Inactivationmentioning

confidence: 99%

The Impact of the Space Environment on Microbial Growth and Behavior

2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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Eliminating transmissibility of bovine spongiform encephalopathy by dry-heat treatment

Cited by 8 publications

References 34 publications

Inactivation of Scrapie Prions by the Electrically Charged Disinfectant CAC-717

Inactivation of Scrapie Prions by the Electrically Charged Disinfectant CAC-717

Heat inactivation of stable proteinaceous particles for future sample return mission architecture

The Impact of the Space Environment on Microbial Growth and Behavior

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