Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Solenostemon scutellarioides

Sahu, Ranabir; Gangopadhyay, Moumita; Dewanjee, Saikat

doi:10.1007/s11738-012-1188-3

Cited by 44 publications

(22 citation statements)

References 33 publications

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“…Similar to the induction of phenylpropanoid biosynthesis, enhanced enzyme activity has been proven. Activity of PAL, cinnamic acid 4-hydoxylase or chalcone synthase and isomerase, as well as the activity of related genes, was all stimulated after MeJA treatment (Sahu et al 2013;Ahn et al 2014). Several enzymes mentioned are also included in the biosynthetic pathway leading to the formation of various coumarins (simplified in Fig.…”

Section: Discussionmentioning

confidence: 96%

“…In addition, MeJAenhanced accumulation of soluble and wall-bound phenols (e.g. hydroxybenzoic acid, derivates of cinnamic and hydoxycinnamic acid) displayed a time dependent (Sircar et al 2012) or a concentration dependent behaviour (Sahu et al 2013). Moreover, coumarins with a higher amount of hydroxyl groups express better scavenging properties than unhydroxylated coumarins (Lin et al 2008).…”

Section: Discussionmentioning

confidence: 99%

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Dynamics of accumulation of coumarin-related compounds in leaves of Matricaria chamomilla after methyl jasmonate elicitation

et al. 2015

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The effect of methyl jasmonate (MeJA) treatment on diploid and tetraploid plants of Matricaria chamomilla and the changes of the main physiological parameters and secondary metabolites content was studied. Leaf rosettes from 7-week-old plants were harvested at four different time points (24, 48, 72, 96 h) after foliar application of 0.4 mM MeJA. The treatment led to a moderate biomass accumulation accompanied by an accumulation of photosynthetic pigments and decrease in the total soluble proteins. The content of hydrogen peroxide and superoxide radical was most elevated at 24 and 72 h after the treatment. Although the values of stress parameters were higher in tetraploid plants (such as in control so in treated plants), their total increase after the MeJA application was similar. The absolute level of total soluble phenols and flavonoids in the controls was similar in both cultivars and their content was enhanced in 24-and 48-h variants, more rapidly in diploid plants. MeJA has been found to trigger different responses of the secondary metabolites accumulation. Almost all studied metabolites were higher in control plants in the tetraploid cultivar, but their biosynthesis was more stimulated in diploids. Significant changes, mainly increasing trends with a maximum between 24 and 48 h after elicitation, in the content of free coumarin herniarin and its glycosidic precursors (Z)-and (E)-2-b-D-glucopyranosyloxy-4-methoxycinnamic acid were observed. The umbelliferone level increased over time. The maximum values for chlorogenic acid and 1,5-dicaffeoylquinic acid were at 24-h and between the 24-72 h time points. Among dicycloethers, the amounts of (E)-form decreased with time, and for (Z)-form an opposite trend was observed, with a maximum of 96 h for diploid and 24 h for tetraploid after elicitation.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Dynamics of accumulation of coumarin-related compounds in leaves of Matricaria chamomilla after methyl jasmonate elicitation

et al. 2015

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“…As elicitors often display a synergistic effect on the production of secondary metabolites when applied in combination (Sahu et al, 2013), the present study also investigates the effects of combined MeJa and SA application on biomass accumulation and the content of iridoids and phenylethanoids in R. glutinosa hairy root cultures (Figs. 2c, d and 4).…”

Section: The Effect Of Meja and Sa Combination On Root Biomass And Mementioning

confidence: 99%

The Influence of Methyl Jasmonate and Salicylic Acid on Secondary Metabolite Production in Rehmannia Glutinosa Libosch. Hairy Root Culture

Piątczak¹,

Kuźma²,

Wysokińska³

2016

Acta Biologica Cracoviensia S. Botanica

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Rehmannia glutinosa hairy roots were used to evaluate the effect of methyl jasmonate (MeJa) and salicylic acid (SA) on increase of root biomass and production of iridoids (catalpol, harpagide) and phenylethanoids (verbascoside and isoverbascoside). The elicitors were added to 23-day-old culture separately at concentrations between 50 and 200 μM or in combinations at concentrations of 50 and 100 μM. Roots were harvested 72 h and 120 h after elicitation. The type of elicitor, its concentration and exposure time were found to strongly affect the content of each analyzed compound. A 72-hour treatment with 200 μM MeJa was the most effective in increase of verbascoside content (60.07 mg·DW -1 equivalent to 845.45 mg·L -1 ) and isoverbascoside (1.77 mg·DW -1 equivalent to 24.94 mg·L -1 ): these respective amounts were roughly 10-and 6.4-fold higher than the control values (unelicited roots). Exposure to 150 μM MeJa provided optimal harpagide content after 72 hours (0.136 mg·DW -1 ; 7.5-fold increase compared to the control), and catalpol content after 120 hours (up to 2.145 mg·DW -1 ). The combination of MeJa and SA also resulted in higher levels of secondary metabolites compared to the control culture, although these levels were lower than those observed for MeJa alone at the optimal concentration and exposure time. SA alone was less efficient in enhancing metabolite production than MeJa.

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“…Recently, Largia et al (2015) also reported a spectacular improvement in bacoside A elicited by a combination of 25 μM MJ and 25 μM SA for about 3 weeks. The combined application of MJ, SA, and yeast extract resulted in the increased production of rosmarinic acid (twofold) in the whole plant culture of Solenostemon scutellarioides (Sahu et al 2013).…”

Section: The Effect Of Mj Sa and Mj + Sa In Enhancing Bilobalide Anmentioning

confidence: 99%

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Sukito¹,

Tachibana

2016

Bioresour. Bioprocess.

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Background: Bilobalide and ginkgolides are reported to be present only in Ginkgo biloba. However, only trace amounts of bilobalide and ginkgolides are contained in the ginkgo leaves. Nowadays, there has been considerable interest in plant cell cultures as a potential alternative to traditional agriculture for the industrial production of secondary metabolites. Much effort has been put into the use of in vitro cultures as one attractive biotechnological strategy for producing bilobalide and ginkgolides of commercial interest. The aim of this study was to enhance the production of bilobalide and ginkgolides A, B, and C in cell cultures of G. biloba using immobilized cell cultures and the process of elicitation.Results: Based on the effect of the immobilization components on the free cell suspension cultures, it was considered that the jute fiber acted as an elicitor and forced the cells to release the product into the culture media. The resulting biomass was approximately 1.4 times higher than in the cell suspension cultures, and the production of bilobalide and ginkgolides A, B, and C increased 5.0, 3.3, 6.1, and 4.1 times, respectively. Eliciting with methyl jasmonate (MJ) and salicylic acid (SA) in the immobilized cells enhanced bilobalide and ginkgolides A, B, and C, compared with the unelicited controls. The highest accumulation was observed using a combination of 0.1 mM MJ + 0.1 mM SA in the immobilized cells, which produced 1.78, 1.95, 2.05, and 2.95 times more bilobalide and ginkgolides A, B, and C, respectively, than the controls. Conclusions:The positive effects of immobilized cell cultures using jute fiber and the synergism effect of SA and MJ on immobilized cells of G. biloba appear to be the optimal conditions for continuous in vitro production for commercial purposes. This is the first report on analyzing the effects of jute fiber as immobilized cell material on G. biloba cell cultures and the synergism of MJ and SA on bilobalide and ginkgolide production.

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Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Solenostemon scutellarioides

Cited by 44 publications

References 33 publications

Dynamics of accumulation of coumarin-related compounds in leaves of Matricaria chamomilla after methyl jasmonate elicitation

Dynamics of accumulation of coumarin-related compounds in leaves of Matricaria chamomilla after methyl jasmonate elicitation

The Influence of Methyl Jasmonate and Salicylic Acid on Secondary Metabolite Production in Rehmannia Glutinosa Libosch. Hairy Root Culture

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

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